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- Table of Contents
PPP1CB / Serine/threonine-protein phosphatase PP1-beta catalytic subunit · Western blot design guide
Design a Western Blot for PPP1CB
Source-linked PPP1CB Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PPP1CB WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
PPP1CB Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~37.2 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Appendix (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked PPP1CB Western Blot Protocol Options
The M04022-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Jurkat cell lysate (catalog M04022-1) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M04022-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected PPP1CB Western Blot Band Size?
PPP1CB is predicted at 37.2 kDa; its annotated modifications could affect migration, but no empirical band or visible shift is established.
| Single band near 37.2 kDa | Consistent with full-length PPP1CB; confirm identity with a specific control |
| Band near 37.2 kDa in cytoplasmic fraction | Consistent with its documented cytoplasmic localization |
| Band near 37.2 kDa in nuclear fraction | Consistent with its documented nuclear localization |
| Possible close doublet near 37.2 kDa | Thr316 phosphorylation could affect migration, but a doublet is not established |
💡Expected PPP1CB appearanceUniProt predicts 37.2 kDa for PPP1CB; no empirical band size is supplied, so confirm a band near that mass with an identity control.
| UniProt predicted mass | Places the full-length protein near 37.2 kDa |
| 327-residue sequence | Defines the full-length protein underlying the 37.2 kDa prediction |
| N-acetylalanine at residue 2 | Is annotated, with no demonstrated visible size shift |
| Phosphothreonine at residue 316 | Could affect migration, but no band shift is demonstrated |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | PPP1CB can occur in cytoplasmic and nuclear compartments | Check both fractions and a suitable positive lysate |
| Band higher than expected | Its identity or migration is uncertain; no glycosylation or disulfide-linked dimer is annotated | Confirm identity with PPP1CB depletion or a second antibody |
| Band lower than expected | Its identity is uncertain; no signal peptide or propeptide cleavage is annotated | Confirm identity with PPP1CB depletion or a second antibody |
| Multiple bands | Thr316 phosphorylation could contribute, but distinct bands are not established | Compare phosphatase-treated samples and confirm band identity |
| Weak or no signal | PPP1CB may be distributed between cytoplasmic and nuclear compartments | Check fraction recovery and a suitable positive lysate |
Experiment evidence
Western blot images showing PPP1CB band
M04022-1 · PPP1CB
A04022-1 · PPP1CB
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PPP1CB Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for PPP1CB in Western blot, you can use appendix tissue, which HPA rates as highly expressed.
Positive control: Appendix (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: PPP1CB is intracellular, and HPA lists not-detected tissues that can serve as negative controls.
HPA tissue expression evidence for PPP1CB
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | endocrine cells | High | Protein (IHC) | HPA → |
| Gallbladder | glandular cells | High | Protein (IHC) | HPA → |
| Rectum | endocrine cells | High | Protein (IHC) | HPA → |
| Small intestine | endocrine cells | High | Protein (IHC) | HPA → |
| Breast | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Hippocampus | glial cells | Not detected | Protein (IHC) | HPA → |
| Parathyroid gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | Not detected | Protein (IHC) | HPA → |
| Spleen | cells in red pulp | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced PPP1CB Western Blot Tips
Deeper troubleshooting and optimisation questions for PPP1CB, answered from its protein features.
What should be checked when unexpected PPP1CB bands appear?
Band shift · The supplied features list no glycosylation sites, signal peptide, propeptide, or alternative sequence. They do not establish a cause for extra bands. Confirm band identity, especially because PP1 also has PPP1CA and PPP1CC catalytic subunits.
Could a second band be a PPP1CB isoform?
Isoforms · The supplied record lists one isoform and no alternative sequence. It provides no isoform-based explanation for a second band; verify band identity before assigning one.
Can PPP1CB modifications explain a shifted band?
PTM · UniProt lists N-acetylalanine at position 2 and phosphothreonine at position 316. These are UniProt coordinates; antibody or paper numbering may differ. Their presence alone does not establish a visible shift or explain a difference from 37.2 kDa.
PPP1CB has a listed phosphothreonine at UniProt position 316. A band at a different apparent mass is not, by itself, evidence of phosphorylation at that site.
PPP1CB has a listed phosphothreonine at UniProt position 316. A band at a different apparent mass is not, by itself, evidence of phosphorylation at that site.
Does this guide establish induction of PPP1CB?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for PPP1CB?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M04022-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should PPP1CB bands be quantified across fractions?
Quantitation · PPP1CB occurs in both cytoplasmic and nuclear compartments and can relocalize. Quantify the same identified band consistently within each fraction; a change in one fraction alone need not represent a change in total cellular PPP1CB.
Should PPP1CB migrate at its predicted 37.2 kDa?
Interpretation · 37.2 kDa is the predicted mass, and no observed band position is supplied. Use it as a reference, but do not assign a band from apparent mass alone.
PPP1CB is reported in cytoplasm and nucleus, including nucleoplasm and nucleolus. It is highly mobile and can relocalize through targeting-subunit interactions, so interpret a fraction-specific signal in the context of the fraction examined.
PPP1CB is reported in cytoplasm and nucleus, including nucleoplasm and nucleolus. It is highly mobile and can relocalize through targeting-subunit interactions, so interpret a fraction-specific signal in the context of the fraction examined.
Boster reagents
PPP1CB Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Protein Phosphatase 1 beta Rabbit Monoclonal Antibody
Cat # M04022-1
Real WB data
Anti-PP1 beta PPP1CB Monoclonal Antibody
Cat # A04022-1
Two the supplier monoclonal anti-PPP1CB antibodies have WB images: M04022-1 in Jurkat lysate and A04022-1 in mouse brain, F9, and MCF7 lysates. These examples do not establish WB performance across every listed reactive species.
Which to pick: Choose M04022-1 for a Jurkat lysate precedent, or A04022-1 for a mouse brain, F9, or MCF7 lysate precedent. A04022-1 lists broader species reactivity, but the supplied WB image does not demonstrate every listed species.
Source: BosterBio PPP1CB gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P62140.
- Human Protein Atlas. PPP1CB tissue expression.
- PMC13464395 — target-verified WB comparison
- PMC4425028 — target-verified WB comparison
- PMC11481231 — target-verified WB comparison