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- Table of Contents
Facts about Protein phosphatase 1 regulatory subunit 14A.
Inhibitor of PPP1CA.
Has over 1000-fold higher inhibitory action when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction (By similarity).. .
Mouse | |
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Gene Name: | Ppp1r14a |
Uniprot: | Q91VC7 |
Entrez: | 68458 |
Belongs to: |
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PP1 inhibitor family |
17 kDa PKC-potentiated inhibitory protein of PP1; CPI-1717-kDa PKC-potentiated inhibitory protein of PP1; CPI1717-KDa protein; PKC-potentiated inhibitory protein of PP1; PPP1INL; Protein kinase C-potentiated inhibitor protein of 17 kDa; protein phosphatase 1 regulatory subunit 14A; protein phosphatase 1, regulatory (inhibitor) subunit 14A
Mass (kDA):
16.649 kDA
Mouse | |
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Location: | 7|7 B1 |
Sequence: | 7; |
If you're looking for high-affinity primary antibodies against PPP1R14A, then you've come to the right place. Boster offers antibodies that recognize PPP1R14A with high specificity, which is vital for applications like cell culture studies. This article will provide more information on this marker, including its applications and sensitivity. Let's get started!
High-affinity primary antibodies to PPP1-related protein 14A (PPP1R14A) are available from Boster Bio. These antibodies are monoclonal or polyclonal and have been tested in IP, WB, and IHC. Listed below are some of the benefits of these antibodies. Flow cytometry is an innovative tool for the study of cells, molecules, or organisms.
Primary antibodies are immunoglobulins that only bind to specific antigens. Their quality is defined by their specificity and affinity. The higher the affinity and specificity, the lower the probability of them binding to unintended antigens. Hence, a high-affinity primary antibody is good for detecting, purifying, and measuring the antigen of interest.
Using a mouse anti-b-actin, GFAP, or NCAM antibody as a negative control, researchers could detect the presence of PPP1R14A in a variety of tissues and cells. Moreover, a 1/800 dilution of Vivo-Morpholinos, a human anti-b-actin antibody, enabled the detection of PPP1R14A in mouse tissues in a luciferase assay.
While mouse monoclonal antibodies remain the most widely used monoclonal antibody, rabbit polyclonal antibodies have been growing steadily in the past five years. Despite their shaky history, rabbit polyclonal antibodies are proving to be a highly reliable choice for research purposes. And, of course, the ability to work with them is a distinct advantage.
These antibodies have been developed using a variety of cell cultures and are highly active in human brain cells. Boster Bio offers high-affinity primary antibodies to PPP1R14A to enable scientists to analyze the protein in the most diverse ways. Using them in the right way can help scientists to identify novel pathogens and autoimmune diseases. However, they cannot detect the presence of disease-causing pathogens and other toxins in the brain.
To conduct cell culture studies using the PPP1R14a marker, spheroid-derived cells were seeded into 96-well ultra-low attachment plates and incubated for 7 days. The cells were fixed in 4% paraformaldehyde and stained with toluitin blue D. After incubation in 4% paraformaldehyde, the cells were washed and air-dried. After removing excess crystal violet solution, cells were counterstained with 1% SDS. After a few days, the cells were counted using an instrument called a Victor plate reader to determine clonogenic growth.
The PPP1R14A marker is an essential component of cell culture studies. Since human embryonic stem cells were first isolated in 1998, the ability to study their properties in vitro has been critical to the development of cell culture. Cell culture studies using the PPP1R14A marker are a useful tool for researchers in the fields of genetics, cell biology, and cancer research. It is also important to remember that PPP1R14A markers are a unique cell marker that can help them find the precise cell type needed to conduct a study.
The PPP1R14A marker has many advantages over other models of cell biology. In addition to allowing the investigator to study specific cell types, the PPP1R14A marker makes it possible to identify physiological requirements for specific cell types. Such studies can include positive effects of growth factors and other growth-promoting substances. Additionally, studies can identify events related to apoptosis and programmed cell death.
The PPP1R14A marker can also be used to determine the effects of certain drugs on cancer cell cultures. Cell culture is essential for developing personalized medicines and the development of new cancer treatments. In addition to advancing cancer treatment, the PPP1R14A marker has many other applications in the field of research. For example, it may allow researchers to develop new treatments based on the information gained.
PP1 is a ubiquitous phosphoprotein that regulates a wide variety of cellular processes, including cell cycle, actomyosin contractility, glycogen metabolism, gene expression, and neuronal signaling. This promiscuous enzyme contains a catalytic subunit which dephosphorylates an enormous array of phosphoproteins. Inhibitory and regulatory subunits control the activity of PP1 enzymes.
The expression of the PPP1R14A marker was found in the developing gut and swim bladder. It was also detected in the ventral mesoderm and hindbrain neural plate, as well as the central nervous system. This marker has many applications in embryology, tissue engineering, and fetal and adult development. Interestingly, the PPP1R14A gene is expressed in both human and mouse neural tissue.
The PPP1R14A gene has homologs in several fish species, including zebrafish and cavefish. Its synteny analysis was conducted using a program called Genomicus. In these examples, the genes are homologous and are shown with a matching coloration. A boxed arrow indicates the direction of gene chromosomes, and the species and chromosome number are indicated.
The PPP1R14A gene is a human-specific protein phosphatase inhibitory subunit. The PPP1R14A gene is enriched for expression in many tissues. It is expressed most abundantly in the heart and testis. Despite the high expression of this gene in humans, it is not widely expressed in other organs. Interestingly, researchers have identified three novel cDNAs that encode the protein. These cDNAs encode putative inhibitory protein, which inhibits the holoenzyme in mouse and human cells.
The PPP1R14A marker is a family of gene markers that has previously been characterized. PPP1R14aa expression has been identified in the developing gut and swim bladder. It has also been identified in the spinal cord and hindbrain oligodendrocytes and is enriched in the rhombomere 4. The PPP1R14B gene is found in the central nervous system and is a marker of intestinal disease.
PMID: 12974676 by Liu Q.-R., et al. GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA.