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- Table of Contents
PRSS1 / Serine protease 1 · Western blot design guide
Design a Western Blot for PRSS1
Source-linked PRSS1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PRSS1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
PRSS1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~26.6 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Duodenum (IHC candidate; verify WB) +2 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Cleaved | |
| Caveat | Zymogen activation controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked PRSS1 Western Blot Protocol Options
The A01637 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | lysate from 293 cells, (catalog A01637) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A01637; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected PRSS1 Western Blot Band Size?
PRSS1 has a predicted 26.6 kDa precursor; secretion and cleavage may affect detection or size, but no empirical migration is supplied.
| Band near 26.6 kDa | consistent with the predicted full-length precursor; confirm identity with controls |
| Band below 26.6 kDa | could reflect signal-peptide or propeptide cleavage |
| Little or no band in whole-cell lysate | consistent with secretion into the extracellular space |
| Two bands near the expected size | could reflect precursor and processed forms; confirm their identities |
💡Expected PRSS1 appearanceUniProt predicts a 26.6 kDa full-length PRSS1 precursor; cleavage may yield a smaller form, but no empirical band size or demonstrated migration shift is supplied.
| UniProt predicted mass | 26.6 kDa for the full-length precursor |
| Signal peptide at residues 1–15 | cleavage can make the secreted form smaller than the precursor |
| Propeptide at residues 16–23 | cleavage can make the active form smaller than the precursor |
| Sulfotyrosine at residue 154 | a modification is annotated, but a visible size shift is not established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | PRSS1 is secreted | probe conditioned medium alongside lysate |
| Band lower than expected | signal-peptide or propeptide cleavage may reduce size | compare precursor and processed-form controls |
| Band higher than expected | retention of the signal peptide or propeptide could increase size relative to a processed form | compare with a full-length precursor control |
| Multiple bands | precursor and processed forms may coexist | check band identity with epitope information and appropriate controls |
| Weak or no signal | secretion may leave little PRSS1 in lysate | test conditioned medium and a positive control |
| Fragments below expected size | proteolysis may generate fragments | use protease inhibitors during preparation and confirm identity with an independent antibody |
Experiment evidence
Western blot images showing PRSS1 band
A01637 · PRSS1
A01637 · PRSS1
A01637-1 · PRSS1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PRSS1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for PRSS1 in Western blot, you can use duodenum tissue.
Positive control: Duodenum (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: Because PRSS1 is secreted, whole-cell lysate may give a weak signal; consider secreted material.
HPA tissue expression evidence for PRSS1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Duodenum | paneth cells | High | Protein (IHC) | HPA → |
| Pancreas | exocrine glandular cells | High | Protein (IHC) | HPA → |
| Small intestine | paneth cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | endocrine cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced PRSS1 Western Blot Tips
Deeper troubleshooting and optimisation questions for PRSS1, answered from its protein features.
How should PRSS1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Are multiple PRSS1 isoforms expected?
Isoforms · The supplied UniProt record lists one isoform and no alternative sequence. Do not assign multiple bands to documented PRSS1 isoforms on this evidence.
Which PRSS1 modification should I consider?
PTM · UniProt lists sulfotyrosine at position 154, using its 247-residue sequence numbering. Antibody or paper numbering may use a different convention. This modification is relevant when interpreting reactivity, but its presence alone does not demonstrate a visible band shift.
Does this guide establish induction of PRSS1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for PRSS1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A01637 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should I choose samples for PRSS1 quantitation?
Quantitation · PRSS1 is annotated as secreted into the extracellular space. Choose a sample fraction that includes secreted protein, and keep the fraction and collection conditions consistent across samples. State whether the measurement represents total detectable PRSS1 or a particular processed form.
Why might PRSS1 migrate differently from its predicted 26.6 kDa?
Interpretation · The 26.6 kDa prediction refers to the full sequence. PRSS1 has a signal peptide at residues 1..15 and a propeptide at 16..23, so processed forms may contain fewer residues. No observed band size is supplied; these features alone do not establish a visible shift or explain a measured difference.
Distinguish the full precursor from forms processed at the signal peptide (1..15) and propeptide (16..23). An antibody’s epitope determines which forms it can detect. The supplied features do not establish which form appears in a sample.
Consider precursor and processed forms because PRSS1 has signal and propeptide regions. It also has five disulfide bonds and interacts with SERPINA1. These features suggest possibilities to investigate, but they do not identify an unexpected band without additional evidence.
Distinguish the full precursor from forms processed at the signal peptide (1..15) and propeptide (16..23). An antibody’s epitope determines which forms it can detect. The supplied features do not establish which form appears in a sample.
Consider precursor and processed forms because PRSS1 has signal and propeptide regions. It also has five disulfide bonds and interacts with SERPINA1. These features suggest possibilities to investigate, but they do not identify an unexpected band without additional evidence.
Boster reagents
PRSS1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Trypsin-1 PRSS1 Antibody
Cat # A01637
Real WB data
Anti-Trypsin-1 PRSS1 Antibody
Cat # A01637-1
The catalog reports two anti-PRSS1 antibodies with WB images. A01637’s caption reports 293-cell lysate; A01637-1’s caption identifies a WB analysis but gives no sample. These captions provide limited validation detail.
Which to pick: Choose A01637 for reported Human or Rat reactivity and a WB image using 293-cell lysate. Choose A01637-1 for reported Human or Mouse reactivity; its WB image caption does not identify the sample.
Source: BosterBio PRSS1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P07477.
- Human Protein Atlas. PRSS1 tissue expression.
- PMC7876278 — target-verified WB comparison
- PMC5424103 — target-verified WB comparison