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- Table of Contents
1 Citations 6 Q&As
Facts about Prostasin.
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Human | |
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Gene Name: | PRSS8 |
Uniprot: | Q16651 |
Entrez: | 5652 |
Belongs to: |
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peptidase S1 family |
CAP1; channel-activating protease 1; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.120; Prostasin; protease, serine, 8; Prss8; Serine protease 8
Mass (kDA):
36.431 kDA
Human | |
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Location: | 16p11.2 |
Sequence: | 16; NC_000016.10 (31131433..31135727, complement) |
Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid.
[Prostasin]: Cell membrane; Single-pass membrane protein.; [Prostasin light chain]: Secreted, extracellular space. Found in the seminal fluid. Secreted after cleavage of its C-terminus.; [Prostasin heavy chain]: Secreted, extracellular space. Found in the seminal fluid. Secreted after cleavage of its C-terminus.
Steven Boster was one of the first entrepreneurs to develop products in the field of immunohistochemistry. He was known as "the man that converts science in a lavatory" because of his work. He was a prolific inventor, creating hundreds upon hundreds of primary antibodies for IHC or ELISA. He was eventually the biggest Chinese antibody catalog business, creating the PicoKine(tm platform) and delivering high-sensitivity ELISA kit kits.
The PRSS8 gene expression pattern was studied in 18 types and matched normal samples of 390 subjects. The PRSS8 gene was significantly overexpressed in tumors as compared to normal ovaries. It was overexpressed 106-fold in OVC compared to epithelial cells from the normal ovaries. PRSS8 was found to be downregulated in stomach, pancreatic, and stomach cancers.
In situ hybridization allows researchers to detect gene activity by using fluorescent dye to label a portion of DNA. This fluorescent dye is then incubated with chromosomes from the origin genome. The DNA bound to the chromosome reveals its location. This technique can also be used to identify other genes, such as those associated with cancer. In situ hybridization can yield reliable results, but it can be expensive to label DNA.
The sequence of the PRSS8 marker was 5'-DICGN-GCAGTAAA-TTGACG-GCTAAGTGTCATCGCA-GCC-GACTG. The hybridization should take place at 37 degrees Celsius overnight. To optimize the temperature of your samples, it may be necessary to perform preliminary experiments. Both microglia and macrophages in SIVE express the PRSS8 gene.
The PRSS8 gene can be detected in many cancer types and tissues. TSA has increased the sensitivity of this method by amplifying the PRSS8 gene. This procedure can be performed using an automated system. The PRSS8 gene was labeled with a fluorescent dye in one study and the results were analyzed in another. These studies have shown that PRSS8 levels are strongly related to the presence cancer.
The current study will provide data and analysis about PRSS8 as an OVC biomarker. This study revealed that PRSS8 expression in OVC tissues is higher than in normal cells. This study also defines its potential application as a biomarker for early detection of OVC. It is one in nine biomarkers to OVC.
The serum samples were separated using SDS-PAGE under reducing conditions, and then stained with Coomassie Blue. The Bradford Assay was used then to determine the protein content. Then, 20mg of total protein were resolved using 12.5 % SDS/PAGE. Bioserve, Proteogenex and patients with OVC obtained sera. Sera from nine healthy donors were also pooled. The PRSS8 immunoreactive antibody was used. The probe sequence was 5'-GCAGTAAAACTCCTGACTCA-CCTTAA-A-G-C-HRP.
Despite the lack of PRSS8 in the skin, the CAP1/PRSS8 mutation mouse model showed no signs that normal differentiation occurred in skin. The skin phenotypes of PRSS8-deficient mice were similar to those of matriptase/MT-SP1-deficient mice, indicating that the proteins of these genes may participate in the same protease-signaling cascade.
Protein expression of PRSS8 in MDCK cells was compared with those of other cell lines. MDCK cells had similar mol weights. MDCK cells also secrete N-deglycosylated and soluble uromodulin. The latter was co-immunoprecipitated with hepsin and prostasin.
DAB chromogenic detector systems are versatile tools for detecting fluorescent proteins in cells. Its color-shifting ability comes from activating NF-kB. This triggers the production and release of alkalinephosphatase. This enzyme causes the protein's color change from pinkish to blue. The DAB chromogenic detection system is designed to detect a variety of fluorescent proteins including DNA and RNA.
PRSS8 is an innovative tumour suppressor. However, it is not clear if this has any clinical significance. Several methods are being tested to determine its expression levels. These methods include gene-specific Amplification and quantitativePCR. This article describes the detection methods of the PRSS8 marker. This research aims provide new insight into PRSS8's biological function and clinical significance in HCC. The next step is to develop a novel diagnostic tool.
To detect the presence of PRSS8 in human tumours, samples were fixed in 10% neutral formalin, embedded in paraffin, and prepared for immunohistochemistry. PRSS8 was detected using immunohistochemistry streptavidin/peroxidase coupling. The tissue was pretreated by 3% H2O2 methane for 10 min. After that, it was incubated 30 minutes with a goat-antiserum and secondary antibody. Finally, it was counterstained with haematoxylin.
PRSS8 protein expression in human tumors is decreased. PRSS8 is associated with a worse prognosis among HCC patients. Its overexpression can lead to a decrease in cell viability. It is an important marker in HCC research, and could also be a potential therapeutic target. This study focuses upon the detection of PRSS8 within HCC tumours.
RTPCR analysis in paired liver and HCC tissues was used to determine PRSS8 levels in different cell kinds. Western blot analysis showed that PRSS8 expression levels were decreased in HCC tissue, but increased in adjacent liver tissues. Inactivating PRSS8 reduced Myst1 transcription. These findings suggest that PRSS8 might negatively correlate to metastatic potential in HCC.
PMID: 7768952 by Yu J.X., et al. Molecular cloning, tissue-specific expression, and cellular localization of human prostasin mRNA.
PMID: 8034638 by Yu J.X., et al. Prostasin is a novel human serine proteinase from seminal fluid. Purification, tissue distribution, and localization in prostate gland.
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