Human Prostasin ELISA Kit PicoKine™

Human Prostasin ELISA Kit PicoKine™ (96 Tests). Quantitate Human PRSS8 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1272
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human Prostasin ELISA Kit PicoKine™

See all Prostasin/Prss8 products

SKU/Catalog Number

EK1272

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Prostasin ELISA Kit PicoKine™ (96 Tests). Quantitate Human PRSS8 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Prostasin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1272)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: A33-G319

Sensitivity

<50 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1272 is reactive to PRSS8 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK1272 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1272-CAP Anti-Human PRSS8 Pre-coated 96-well strip microplate 1
EK1272-ST Human PRSS8 Standard 2 vials, 100 ng/tube
EK1272-DA Human PRSS8 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.126.2512.52550100
O.D.0.0190.3290.5580.7850.9901.2661.5161.625

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Prostasin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3254123964136831151325140618
Standard deviation247.35951944.29267.89689.052599.55
CV (%)7.6%4.8%4.7%8.6%5.2%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Prostasin/Prss8 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 132543608334030363309204.886.1%
Sample 21239612322145961264912990934.687.1%
Sample 34136843230430724305242680760.91.7%
*number of samples for each test n=16.

Gene/Protein Information For PRSS8 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16651

Gene ID

5652

Gene Name

PRSS8

Full Name

Prostasin

Weight

36.431kDa

Superfamily

peptidase S1 family

Alternative Names

CAP1; channel-activating protease 1; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.120; Prostasin; protease, serine, 8; Prss8; Serine protease 8 PRSS8 CAP1, PROSTASIN serine protease 8 prostasin|channel-activating protease 1|channel-activating serine protease 1|protease, serine 8

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PRSS8, check out the PRSS8 Infographic

PRSS8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PRSS8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1272

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6 Customer Q&As for Human Prostasin ELISA Kit PicoKine™

Question

Q: which procedure should I follow in order to thaw whole blood sample for Prostasin ELISA after freezing?

H. Hall

Verified customer

Asked: 2020-05-25

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Prostasin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-05-25

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

P. Allen

Verified customer

Asked: 2020-02-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-02-26

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Prostasin using the EK1272 Human Prostasin Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-10-01

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-11-17

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-11-17

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-10-06

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-10-06

Question

Q: Can Prostasin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2015-08-06

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2015-08-06

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