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- Table of Contents
PSMB10 / Proteasome subunit beta type-10 · Western blot design guide
Design a Western Blot for PSMB10
Source-linked PSMB10 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PSMB10 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
PSMB10 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~28.9 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Bronchus (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Processing-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked PSMB10 Western Blot Protocol Options
The M04960 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Raji cell lysate (catalog M04960) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M04960; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected PSMB10 Western Blot Band Size?
PSMB10 has a predicted 28.9 kDa precursor; propeptide removal may lower its mass, but no empirical migration or distinct band size is supplied.
| Band near 28.9 kDa | consistent with the predicted full-length precursor, pending identity controls |
| Band below 28.9 kDa | could reflect removal of the 1–39 propeptide |
| Upper and lower bands | could represent precursor and processed PSMB10 |
| Lower band without a precursor band | could reflect predominantly processed PSMB10 |
💡Expected PSMB10 appearanceUniProt predicts a 28.9 kDa full-length precursor; removal of its 1–39 propeptide may yield a smaller band, but migration has not been measured here, so confirm band identity with appropriate controls.
| Predicted full-length mass | places the 273-residue precursor at 28.9 kDa |
| Propeptide at residues 1–39 | contributes to precursor mass before processing |
| Propeptide cleavage | removes residues 1–39 and lowers mature protein mass |
| Precursor and mature forms | could produce bands at different sizes if both are present |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | the 1–39 propeptide may remain on the precursor | compare with a validated PSMB10 positive control |
| Band lower than expected | the 1–39 propeptide may have been removed | check whether the band is consistent with processed PSMB10 using an independent antibody |
| Multiple bands | precursor and processed forms may coexist | compare bands with an independent PSMB10 antibody and a positive control |
| Weak or no signal | PSMB10 may be below detection in the tested lysate | check loading and use a validated positive lysate |
| Fragments below expected size | additional breakdown during sample preparation is possible | prepare fresh lysate with protease inhibitors and compare with a positive control |
Experiment evidence
Western blot images showing PSMB10 band
M04960 · PSMB10
M04960-1 · PSMB10
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PSMB10 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for PSMB10 in Western blot, you can use bronchus tissue, which HPA rates as high expression.
Positive control: Bronchus (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: Tissue controls are feasible because HPA reports high expression in bronchus and no detection in adipose tissue.
HPA tissue expression evidence for PSMB10
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bronchus | ciliated cells (cilia axoneme) | High | Protein (IHC) | HPA → |
| Duodenum | glandular cells | High | Protein (IHC) | HPA → |
| Fallopian tube | ciliated cells (cilia axoneme) | High | Protein (IHC) | HPA → |
| Kidney | cells in glomeruli | High | Protein (IHC) | HPA → |
| Lung | alveolar cells type I | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
| Cerebellum | cells in granular layer | Not detected | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced PSMB10 Western Blot Tips
Deeper troubleshooting and optimisation questions for PSMB10, answered from its protein features.
How should PSMB10 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Are multiple PSMB10 isoforms expected?
Isoforms · The supplied UniProt record lists one isoform and no alternative sequence. Do not assign multiple bands to splice isoforms on this evidence; check whether the antibody recognizes precursor or processed PSMB10.
Can PSMB10 modifications explain a shifted band?
PTM · UniProt lists N-acetylmethionine at residue 1 and phosphoserine at residue 230, using UniProt sequence numbering. Residue 1 lies within the annotated propeptide (1..39). Neither modification establishes a visible shift or explains a measured mass difference without further evidence.
Does this guide establish induction of PSMB10?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for PSMB10 Western blot?
Transfer · UniProt does not specify a transfer method. Assess transfer around the 28.9-kDa precursor and any smaller processed form expected from the 1..39 propeptide annotation. Check protein retention and transfer before interpreting a missing band.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M04960 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
Which PSMB10 form should be quantified?
Quantitation · Define whether the readout is precursor PSMB10, processed PSMB10, or both. The annotated 1..39 propeptide makes that distinction relevant. Apply the same band definition across samples and report it with the quantitation.
Why might PSMB10 migrate below its predicted 28.9 kDa?
Interpretation · The 28.9-kDa prediction is for the 273-residue sequence, which includes a propeptide at UniProt residues 1..39. Processing could produce a smaller form. No observed band size is supplied, so the feature alone cannot establish the size or identity of a band.
UniProt places PSMB10 in the cytoplasm and nucleus. If comparing fractions, assess each fraction consistently and identify the band being measured; a change in one fraction alone does not establish a change in total PSMB10.
Consider the annotated 1..39 propeptide when evaluating smaller bands. PSMB10 is also a subunit of the proteasome core, so assay conditions can matter when interpreting higher-mass signals. The record provides no observed band size; confirm band identity before assigning processing, modification, or assembly.
UniProt places PSMB10 in the cytoplasm and nucleus. If comparing fractions, assess each fraction consistently and identify the band being measured; a change in one fraction alone does not establish a change in total PSMB10.
Consider the annotated 1..39 propeptide when evaluating smaller bands. PSMB10 is also a subunit of the proteasome core, so assay conditions can matter when interpreting higher-mass signals. The record provides no observed band size; confirm band identity before assigning processing, modification, or assembly.
Boster reagents
PSMB10 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-PSMB10 Rabbit Monoclonal Antibody
Cat # M04960
Real WB data
Anti-PSMB10 Rabbit Monoclonal Antibody
Cat # M04960-1
Two the supplier rabbit monoclonal anti-PSMB10 antibodies have Western blot images: M04960 in Raji cell lysate and M04960-1 in Daudi cell lysate. These images document those tested samples; the supplied evidence does not establish performance in other samples.
Which to pick: For a human sample, either listed antibody has a WB image. M04960 also lists mouse and rat reactivity; M04960-1 lists human only. Choose based on the reported reactivity and which tested lysate is closer to your experiment.
Source: BosterBio PSMB10 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P40306.
- Human Protein Atlas. PSMB10 tissue expression.
- PMC12131556 — target-verified WB comparison
- PMC7102838 — target-verified WB comparison
- PMC12616017 — target-verified WB comparison