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- Table of Contents
PTER / N-acetyltaurine hydrolase · Western blot design guide
Design a Western Blot for PTER
Source-linked PTER Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PTER WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
PTER Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~39 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Colon (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked PTER Western Blot Protocol Options
The A08763 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human kidney tissue lysate (catalog A08763) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A08763 · (A) 1 and (B) 2 μg/mL (catalog A08763) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected PTER Western Blot Band Size?
PTER is predicted at 39 kDa; two annotated isoforms could affect migration, but no distinct bands or empirical size are established.
| Single band near 39 kDa | consistent with the predicted PTER mass; identity requires controls |
| Two bands at different sizes | could reflect isoforms 1 and 2; their migration is not established |
| One band despite two annotated isoforms | the isoforms may not resolve or both may not be detected |
| Band in a cytosolic fraction | consistent with PTER's annotated cytosolic location |
💡Expected PTER appearanceUniProt predicts PTER at 39 kDa; no empirical band size or isoform migration is supplied, so confirm a candidate band with antibody specificity controls.
| UniProt predicted mass | places the reference protein near 39 kDa |
| 349 amino acid reference sequence | underlies the 39 kDa predicted mass |
| Isoform 1 | may migrate differently from isoform 2; its size is unspecified |
| Isoform 2 | may migrate differently from isoform 1; its size is unspecified |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | cytosolic PTER may be poorly recovered or below detection | check cytosolic recovery and use a positive control lysate |
| Band higher than expected | isoform migration or band identity is unresolved | compare with a PTER positive control and validate specificity |
| Band lower than expected | isoform migration or band identity is unresolved | compare with a PTER positive control and validate specificity |
| Multiple bands | two annotated isoforms are possible, but their separation is unknown | check antibody specificity and compare across controlled samples |
| Weak or no signal | PTER abundance or cytosolic recovery may be low | check loading, cytosolic recovery, and a positive control |
Experiment evidence
Western blot images showing PTER band
A08763 · PTER
A08763-1 · PTER
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PTER Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for PTER in Western blot, you can use colon lysate, where HPA reports high expression.
Positive control: Colon (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: Cytosolic PTER should be accessible in tissue lysates, though adipose tissue may give little or no signal.
HPA tissue expression evidence for PTER
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Colon | glandular cells | High | Protein (IHC) | HPA → |
| Fallopian tube | glandular cells | High | Protein (IHC) | HPA → |
| Gallbladder | glandular cells | High | Protein (IHC) | HPA → |
| Kidney | cells in tubules | High | Protein (IHC) | HPA → |
| Pancreas | exocrine glandular cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Ovary | ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | smooth muscle cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced PTER Western Blot Tips
Deeper troubleshooting and optimisation questions for PTER, answered from its protein features.
Where should the main PTER band appear?
Band shift · Use 39 kDa, the supplied predicted mass, as a starting reference. No observed band position is available, so compare the sample with a molecular weight marker rather than assuming an exact apparent mass.
Could PTER isoforms produce different bands?
Isoforms · Two isoforms are listed. Isoform 2 lacks residues 234–280 in UniProt numbering, so its sequence is shorter than isoform 1. Whether the isoforms resolve as separate bands depends on the assay; their observed band positions are not supplied.
Check the antibody epitope against UniProt residues 234–280. An epitope entirely within that segment would be absent from isoform 2; an epitope shared by both isoforms could detect both.
Determine whether one or two bands are resolved before choosing a measurement. For isoform-specific quantitation, measure resolved bands separately and confirm that the antibody recognizes each intended isoform. If they overlap, report the signal as combined PTER rather than assigning it to one isoform.
Check the antibody epitope against UniProt residues 234–280. An epitope entirely within that segment would be absent from isoform 2; an epitope shared by both isoforms could detect both.
Determine whether one or two bands are resolved before choosing a measurement. For isoform-specific quantitation, measure resolved bands separately and confirm that the antibody recognizes each intended isoform. If they overlap, report the signal as combined PTER rather than assigning it to one isoform.
Should glycosylation or another listed modification explain a shifted band?
PTM · The supplied features list no glycosylation sites or modified residues. Do not assign a shifted band to a specific modification from these data. Even a listed modification would not, by itself, establish a visible shift.
Does this guide establish induction of PTER?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for PTER?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A08763 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should PTER be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Which fraction is appropriate for detecting PTER?
Interpretation · PTER is annotated in the cytoplasm, specifically the cytosol. A cytosolic fraction is therefore a reasonable sample to examine, while checking recovery during fractionation.
Compare its position with the 39 kDa predicted mass and consider the shorter isoform 2, which lacks UniProt residues 234–280. The features do not establish either isoform’s observed mobility or identify other bands; confirm band identity with an appropriate specificity control.
Compare its position with the 39 kDa predicted mass and consider the shorter isoform 2, which lacks UniProt residues 234–280. The features do not establish either isoform’s observed mobility or identify other bands; confirm band identity with an appropriate specificity control.
Boster reagents
PTER Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-PTER Antibody
Cat # A08763
Real WB data
Anti-PTER Antibody Picoband®
Cat # A08763-1
Two the supplier anti-PTER antibodies are listed with human, mouse, and rat reactivity and WB images. The images show human kidney lysate for A08763 and rat and mouse kidney lysates for A08763-1. No publication evidence is supplied.
Which to pick: For a documented human kidney example, see A08763 at 1–2 μg/mL. For rat or mouse kidney examples, see A08763-1 at 0.5 μg/mL, with a reported band near 39 kDa. Both list reactivity to all three species.
Source: BosterBio PTER gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q96BW5.
- Human Protein Atlas. PTER tissue expression.
- PMC4012828 — target-verified WB comparison