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RBBP4 / Histone-binding protein RBBP4 · IHC design guide
Design Immunohistochemistry for RBBP4
Plan chromogenic RBBP4 IHC in paraffin sections around its ubiquitous nuclear tissue pattern (HPA tissue IHC). Use positive tissue controls and interpret staining with the reported possibility that the antibody targets proteins from more than one gene (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
RBBP4 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Ubiquitous nuclear staining (HPA tissue IHC) | |
| Staining pattern | Nuclear staining across many tissue cell types (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet M02702) | |
| Positive control | Adrenal gland+4 more · see all | |
| Negative control | None in HPA (detected in all 45 tissues); use no-primary + isotype controls |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image M02702); optimize if staining varies. | |
| Caveat | Staining may reflect proteins from more than one gene (HPA tissue IHC) | |
| Regulation | Expression regulation not specified (UniProt) | |
| Isoform / epitope | 4 isoforms; epitope coverage is unspecified (UniProt) |
Section 1
Recommended RBBP4 IHC & IF Protocols
The catalog antibody’s IHC-P protocol is accompanied by published RBBP4 staining conditions for mouse brain, mouse liver, and human glioma (datasheet M02702; PMC7113115; PMC9134917; PMC10469049).
| Sample | Paraffin-embedded human lung cancer tissue; fixative not specified (datasheet M02702) |
| Fixation | Image fixative and duration unreported (datasheet M02702); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet M02702); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet M02702) |
| Primary antibody | Rabbit monoclonal (clone AEII-18) anti-RBBP4, 1:50 (datasheet M02702) |
| Primary incubation | Overnight at 4 °C (datasheet M02702) |
| Detection | HRP-conjugated secondary, DAB chromogen (datasheet M02702) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | RBBP4-positive staining in glandular cells of adrenal gland (HPA tissue IHC: High). HPA tissue profile: Ubiquitous nuclear expression. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA pH 8.0 retrieval for the catalog antibody (datasheet M02702); optimize retrieval for each tissue and antibody.
Section 2
What Is the Expected RBBP4 Staining Pattern?
RBBP4 is a nuclear, chromatin-associated protein with no transmembrane segment (UniProt Q09028: subcellular location and topology). Expect nuclear staining across many cell types: HPA describes ubiquitous nuclear expression and low tissue specificity, with high staining in breast glandular cells, bone marrow hematopoietic cells, and cerebral cortex glial cells (HPA tissue IHC). Tissue IHC reliability is Supported, with a warning that the staining may reflect proteins from more than one gene (HPA tissue IHC).
| Distinct nuclear staining in glandular cells, hematopoietic cells, or glial cells. | This fits the reported pattern: HPA records high staining in breast glandular cells, bone marrow hematopoietic cells, and cerebral cortex glial cells (HPA tissue IHC). Assess nuclei within their tissue context; the examples do not define an exclusive list of positive cells (HPA tissue IHC: ubiquitous nuclear expression). |
| Signal is predominantly cytoplasmic or outlines cell membranes. | This conflicts with the expected nuclear compartment (UniProt Q09028: nucleus; HPA tissue IHC: ubiquitous nuclear expression). Check staining controls and detection conditions before assigning it to RBBP4. UniProt reports no transmembrane segment, so a membrane pattern lacks support from the supplied target record (UniProt Q09028: topology). |
| Strong staining appears in an unexpected cell population while expected nuclei are weak. | Treat the result as uncertain, not proof of a new cell-specific pattern. Compare nearby expected nuclei and controls; cross-reactivity or endogenous detection activity are possible explanations (general IHC practice). HPA reports ubiquitous nuclear expression and cautions that its tissue staining may reflect proteins from more than one gene (HPA tissue IHC). |
| Color is spread across nuclei, cytoplasm, and tissue spaces without clear cell boundaries. | Diffuse background limits localization and intensity scoring (general IHC practice). Review the no-primary control, blocking, washes, and chromogen development before interpreting weak nuclear color. The supported reference pattern is nuclear, although HPA's antibody warning limits target-specific attribution (HPA tissue IHC). |
| No nuclear signal appears in a section expected to stain strongly. | First verify that the expected cell population is present and that the detection run worked (general IHC practice). Bone marrow hematopoietic cells and breast glandular cells are reported as high-staining examples (HPA tissue IHC). A blank result alone cannot distinguish sample or workflow failure from antibody performance. |
💡Expected RBBP4 appearanceCall a convincing positive result when identifiable cells show predominantly nuclear chromogenic signal, including strong staining in an HPA high-staining population; broad cytoplasmic color or cell-free deposits should prompt a background check (HPA tissue IHC; general IHC practice).
| Compartment and topology | RBBP4 is assigned to the nucleus and telomeric chromosomes and localizes to chromatin as part of PRC2 (UniProt Q09028: subcellular location). Its lack of a transmembrane segment supports using nuclear localization, rather than a membrane outline, as the primary slide-level expectation (UniProt Q09028: topology). |
| Tissue distribution | HPA reports ubiquitous nuclear expression and low tissue specificity, with high staining in several listed populations (HPA tissue IHC). An unlisted cell type is therefore not automatically a negative control; judge an apparent mismatch against compartment, local tissue context, and controls (HPA tissue IHC; general IHC practice). |
| Antibody interpretation | Tissue IHC is rated Supported, but HPA warns that staining may reflect proteins from more than one gene (HPA tissue IHC). HPA060724 and CAB006264 have Supported IHC status; HPA060710 has no listed IHC status (HPA antibodies). A nuclear pattern alone cannot establish unique recognition of RBBP4. |
| IF/ICC Q&A | Where should IF/ICC signal appear? Mainly in the nucleoplasm (HPA subcellular ICC-IF). This is a localization cross-check for the IHC interpretation, not an IHC-P protocol condition; the supplied HPA ICC-IF record does not specify an IHC staining procedure. |
| Situation | Likely cause | Next action |
|---|---|---|
| Expected positive nuclei are blank. | The expected cells may be absent from the section, or a staining step may have failed (general IHC practice). | Confirm tissue identity and inspect a same-run control containing an HPA high-staining population, such as bone marrow hematopoietic cells (HPA tissue IHC). Then review the IHC-P antibody and detection conditions used; target-specific fixation sensitivity is unreported in the supplied sources. |
| Nuclei stain weakly in every section. | Low signal can reflect the staining workflow or detection sensitivity (general IHC practice); the supplied sources do not establish an RBBP4-specific retrieval or fixation effect. | Check the positive control and the antibody's validated IHC-P instructions, then review retrieval, incubation, and detection as general IHC-P variables. Avoid declaring the tissue negative from intensity alone (general IHC practice). |
| Cytoplasm or membranes stain more strongly than nuclei. | The pattern conflicts with RBBP4's nuclear assignment and lack of a transmembrane segment (UniProt Q09028: location and topology). Background or off-target binding is possible (general IHC practice). | Compare with the no-primary control and an expected nuclear-positive section; review blocking and antibody conditions before scoring the cytoplasmic signal (general IHC practice; HPA tissue IHC). |
| The whole section develops diffuse brown color. | Nonspecific background or endogenous detection activity can obscure nuclear staining (general chromogenic IHC practice). | Inspect no-primary and detection controls, check blocking and wash steps, and assess chromogen development. Score RBBP4 only where nuclei remain distinguishable (general IHC practice; HPA tissue IHC: nuclear profile). |
| Unexpected cells show strong nuclear staining. | Nuclear location is compatible with RBBP4, but it cannot establish target specificity; HPA cautions that tissue staining may reflect more than one gene (HPA tissue IHC). | Compare the same run with known high-staining cells and relevant controls. Report the unexpected population as an observation pending specificity checks, especially because HPA describes broad nuclear expression (HPA tissue IHC; general IHC practice). |
| A proposed antibody has only ICC support. | ICC support does not supply IHC-P validation: HPA060710 is ICC Supported and has no listed IHC status (HPA antibodies). | For paraffin-section interpretation, use an antibody with documented IHC support, such as HPA060724 or CAB006264, and follow its IHC-P instructions (HPA antibodies; general IHC practice). |
Experiment evidence
IHC & IF images showing RBBP4 staining
M02702 · IHCIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). RbAp48 was detected in a paraffin-embedded section of human lung cancer tissue.
M02702 · IHCIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). RbAp48 was detected in a paraffin-embedded section of human lymphoma tissue. Hea
M02702-1 · IHCIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). RbAp48 was detected in paraffin-embedded section of human intestinal cancer ti
M02702-1 · IHCIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). RbAp48 was detected in paraffin-embedded section of human placenta tissue . He
PB9797 · IHCIHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797). RbAp48 was detected in paraffin-embedded section of Human Intestinal Cancer Tiss
PB9797 · IHCIHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797). RbAp48 was detected in frozen section of mouse small intestine tissue. The tissu
PB9797 · IFIF analysis of RbAp48 using anti-RbAp48 antibody (PB9797) and anti-Beta Tubulin antibody (M01857-3). RbAp48 was detected in immunocytochemic
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000162521-RBBP4/tissue/cerebral+cortex#imid_17209987
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000162521-RBBP4/tissue/colon#imid_17209897
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000162521-RBBP4/tissue/liver#imid_17209914
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000162521-RBBP4/tissue/kidney#imid_17209915
HPA · ICC-IF HEK293Human Protein Atlas immunofluorescence, HEK293, antibody HPA060710; green = RBBP4, blue = nucleus, red = microtubules.
HPA · ICC-IF HEK293Human Protein Atlas immunofluorescence, HEK293, antibody HPA060710; green = RBBP4, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for RBBP4 IHC & IF
🧪Run bone marrow first: hematopoietic cells should show strong RBBP4 staining (HPA: High in bone marrow hematopoietic cells). HPA detects RBBP4 in all 45 scored tissues, so there is no supported negative tissue; use no-primary and isotype controls, and treat cells within the positive slide as internally negative only if their nuclei show background-level signal (HPA: no negative tissue listed; standard IHC scoring practice).
Positive control tissue: Adrenal gland (Glandular cells, HPA High)
Negative control tissue: None in HPA: RBBP4 is detected in all 45 scored tissues. Use a no-primary (secondary-only) and an isotype control instead.
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show RBBP4 in HEK293, HeLa, U2OS, A-431, U-251MG, with annotated localisation: Nucleoplasm (enhanced) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only slide and a matched rabbit isotype control with the same clonality as the primary; use RBBP4 knockout material as a biological specificity control if available (M02702 caption: rabbit primary; standard IHC control practice). Quench endogenous peroxidase and check for residual signal in bone marrow before interpreting DAB staining (M02702 caption: HRP/DAB detection; standard IHC practice).
⚠️Feasibility: No target-specific fixation window or fixation effect is reported, and the selected M02702 paraffin-section caption does not state a fixative (M02702 caption: fixative unreported). That caption uses heat retrieval in EDTA at pH 8.0, but it does not establish that retrieval is required for every antibody or specimen (M02702 caption: EDTA retrieval). Frozen-section performance is unreported; HPA shows nucleoplasmic ICC-IF signal, but the supplied evidence does not establish that IF is easier than paraffin IHC (HPA: nucleoplasm enhanced; M02702 caption: paraffin-section IHC).
HPA tissue IHC evidence for RBBP4
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Supported — High consistency between antibody staining and RNA expression data. Caution, targets protein from more than one gene.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Glandular cells | High | Protein (IHC) | HPA → |
| Appendix | Glandular cells | High | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | High | Protein (IHC) | HPA → |
| Breast | Glandular cells | High | Protein (IHC) | HPA → |
| Bronchus | Respiratory epithelial cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| None in HPA: RBBP4 is detected in all 45 scored tissues. Use a no-primary (secondary-only) and an isotype control instead. | ||||
Section 3
Advanced RBBP4 IHC Tips
Use nuclear staining, documented retrieval conditions, and matched controls to troubleshoot RBBP4 in paraffin sections (UniProt Q09028 localisation; datasheet M02702).
How should I troubleshoot weak RBBP4 staining after antigen retrieval?
Start with heat-mediated retrieval in EDTA at pH 8.0 for paraffin sections (datasheet M02702). The selected tissue image used this retrieval before overnight incubation with the catalog antibody at 1:50 and 4°C (datasheet M02702). If nuclear staining remains weak, compare a modestly longer heating interval with the original interval on adjacent sections while keeping detection and exposure to DAB constant (standard IHC practice; UniProt Q09028 localisation). Include a known staining section in each run, since a failed retrieval run can resemble absent antigen (standard IHC practice). Evaluate nuclear detail and tissue integrity alongside signal; excessive heating can damage morphology (standard IHC practice).
How can I assess whether fixation is causing weak nuclear staining?
Target-specific fixation sensitivity is unknown: the selected paraffin-section caption does not state a fixative (datasheet M02702). Record the fixative, fixation duration, tissue thickness, and processing history for each specimen before comparing staining intensity (standard IHC practice). On serial sections, hold EDTA retrieval at pH 8.0, primary antibody at 1:50, and detection conditions constant while comparing specimens with documented processing (datasheet M02702; standard IHC practice). Examine nuclear morphology as well as signal, because poorly preserved sections can undermine compartment-based scoring (standard IHC practice; UniProt Q09028 localisation). Do not assign a weak result to a particular fixation effect without a controlled comparison (standard IHC practice).
What staining pattern should count as RBBP4 in a paraffin section?
Score convincing signal in intact nuclei: RBBP4 is nuclear and chromatin-associated, with a telomere annotation (UniProt Q09028 localisation). Human tissue staining is described as ubiquitously nuclear, while cell imaging places the main signal in the nucleoplasm (HPA tissue IHC; HPA subcellular). Compare DAB deposits with a hematoxylin counterstain to check whether staining follows nuclei rather than section folds or pigment (standard IHC practice). Predominantly diffuse cytoplasmic staining calls for review of antibody concentration, blocking, and detection controls before assigning it to RBBP4 (UniProt Q09028 localisation; standard IHC practice). A telomere annotation does not require visible puncta in routine chromogenic sections (UniProt Q09028 localisation; standard IHC practice).
Could isoforms or epitope access explain uneven RBBP4 staining?
RBBP4 has 4 annotated isoforms, so establish which sequence the antibody recognizes before interpreting differences between specimens (UniProt Q09028 isoforms; standard IHC practice). The supplied caption documents tissue staining but does not identify an epitope or establish recognition of every isoform (datasheet M02702). RBBP4 also has annotated acetylated and phosphorylated residues, including phosphoserines at 110 and 355; their effect on this antibody is unknown (UniProt Q09028 modified residues). Compare adjacent sections under the same pH 8.0 EDTA retrieval conditions, and use an independently validated reagent if epitope dependence matters (datasheet M02702; standard IHC practice). Avoid attributing a staining change to an isoform or modification without direct validation (standard IHC practice).
How should I adapt the nuclear readout to multiplex IF?
Treat IF conditions as a separate validation task: the selected antibody evidence describes chromogenic staining of a paraffin section (datasheet M02702). For a bronchus example, pair RBBP4 with a respiratory epithelial cell marker such as cytokeratin and a nuclear counterstain, then assess nuclear signal within the marked cells (HPA: High in bronchus respiratory epithelial cells; UniProt Q09028 localisation; standard IF practice). Choose a far-red fluorophore when shorter-wavelength tissue autofluorescence obscures signal, and inspect unstained and single-label controls (standard IF practice). Because RBBP4 has no transmembrane segment and is nuclear, test gentle permeabilisation, such as 0.1% Triton X-100, when staining fixed cells (UniProt Q09028 topology and localisation; standard IF practice). Optimise that step against nuclear morphology and background rather than transferring the IHC dilution directly (standard IF practice).
What should I check when DAB appears outside nuclei?
First separate diffuse DAB, tissue pigment, and signal at damaged section edges from staining within intact nuclei (standard IHC practice; UniProt Q09028 localisation). The documented tissue workflow used 10% goat serum blocking, a 1:50 primary dilution, a peroxidase-conjugated secondary, and DAB development (datasheet M02702). If background is widespread, compare a no-primary control, check the peroxidase block, and shorten DAB development while keeping the positive control in the run (standard IHC practice). Recheck wash steps and antibody dilution against nuclear contrast; stronger overall brown staining does not establish better RBBP4 detection (standard IHC practice; UniProt Q09028 localisation). Interpret residual cytoplasmic signal cautiously until controls resolve its source (standard IHC practice).
How should I quantify RBBP4 across tissue sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and exclude folds, necrosis, and poorly preserved edges before scoring (standard IHC practice). For intact nuclei, record the percentage positive and an intensity category, then calculate an H-score as the sum of percentage at each intensity multiplied by its 0–3 category (standard IHC practice; UniProt Q09028 localisation). Normalise to the number of eligible nuclei in the same annotated region, and report the region, threshold, and replicate sections (standard IHC practice). If density is the endpoint, report positive nuclei per mm² of viable tissue rather than raw counts (standard IHC practice). Keep retrieval, DAB development, and counterstain consistent across compared slides (standard IHC practice).
How can I distinguish true RBBP4 positivity from staining artefact?
A credible result follows intact nuclei and remains interpretable against the hematoxylin counterstain (UniProt Q09028 localisation; standard IHC practice). High staining has been reported in bronchus respiratory epithelial cells and bone marrow hematopoietic cells, while the broader tissue profile is ubiquitously nuclear (HPA tissue IHC). Use those patterns as context, then verify unusual cell populations with morphology and an appropriate cell marker (HPA tissue IHC; standard IHC practice). Distrust signal confined to edges, necrotic areas, or the wrong compartment, and check a no-primary control for endogenous enzyme or detection background (standard IHC practice; UniProt Q09028 localisation). HPA labels its tissue evidence Supported but cautions that its antibody targets protein from more than one gene (HPA tissue IHC).
Boster reagents
Best RBBP4 / Histone-binding protein RBBP4 IHC Antibodies
Anti-RBBP4 antibodies have IHC images from human paraffin sections and mouse and rat frozen sections, plus IF images from human cells (catalog image captions).
Real IHC data
Anti-RbAp48 Rabbit Monoclonal Antibody
Cat # M02702
Real IHC data
Anti-RbAp48 RBBP4 Antibody ® (monoclonal, 9F3)
Cat # M02702-1
Real IHC data
Anti-RbAp48/RBBP4 Antibody ®
Cat # PB9797
M02702 has paraffin-section IHC images from human lung cancer, lymphoma, glioma, and testis cancer; M02702-1 has paraffin-section IHC images from human intestinal cancer, mammary cancer, and placenta (catalog IHC captions). PB9797 has IHC images from human intestinal cancer paraffin sections and mouse and rat frozen sections, plus ICC/IF images from U2OS and A431 cells (catalog IHC/IF captions).
Which to pick: For paraffin-section IHC, choose rabbit monoclonal M02702 with 1:50 and EDTA pH 8.0 retrieval in its human lung cancer image, or mouse monoclonal M02702-1 with 2 μg/ml and citrate pH 6 retrieval in its human intestinal cancer image; neither caption reports the fixative (M02702 and M02702-1 IHC captions; catalog host and clone fields). For IF/ICC, choose PB9797, which has images from U2OS and A431 cells at 2 μg/ml (PB9797 IF captions). For cross-species tissue IHC, PB9797 has frozen-section images from mouse and rat tissues and a paraffin-section image from human intestinal cancer; its paraffin caption does not report the fixative (PB9797 IHC captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q09028 (RBBP4_HUMAN, Histone-binding protein RBBP4).
- Human Protein Atlas. RBBP4 tissue IHC expression (reliability: Supported).
- Human Protein Atlas. RBBP4 subcellular location (ICC-IF): Mainly localized to the nucleoplasm..
- Human Protein Atlas. RBBP4 antibody validation summary (3 antibodies).
- Loss of Immunohistochemical Reactivity in Association With Handling-Induced Dark Neurons in Mouse Brains. Toxicologic pathology 2020 — PMC7113115.
- Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism. International journal of biological sciences 2022 — PMC9134917.
- Expression and clinical significance of RBBP4 gene in lower-grade glioma: An integrative analysis. Biochemistry and biophysics reports 2023 — PMC10469049.
- Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4. Nature communications 2018 — PMC6053364.
- PubMed PMID:8350924 — UniProt-cited evidence.
- PubMed PMID:14702039 — UniProt-cited evidence.
- PubMed PMID:16710414 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for RBBP4 (Histone-binding protein RBBP4). Expected localisation Ubiquitous nuclear staining (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet M02702). Validated IHC antibody: M02702 at 1:50 (the product's own IHC validation image is shown). Controls (HPA): positive Adrenal gland (Glandular cells); negative None in HPA (detected in all 45 tissues); use no-primary + isotype controls. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human lung cancer tissue; fixative not specified (datasheet M02702); 2. Fixation: Image fixative and duration unreported (datasheet M02702); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet M02702); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet M02702); 8. Primary antibody: Rabbit monoclonal (clone AEII-18) anti-RBBP4, 1:50 (datasheet M02702); 9. Primary incubation: Overnight at 4 °C (datasheet M02702). Watch for: Fixation: Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image M02702); optimize if staining varies.; Caveat: Staining may reflect proteins from more than one gene (HPA tissue IHC); Isoform / epitope: 4 isoforms; epitope coverage is unspecified (UniProt); Regulation: Expression regulation not specified (UniProt). Evidence: UniProt Q09028, HPA tissue IHC · Supported, HPA ICC-IF · Mainly localized to the nucleoplasm., Datasheet M02702, PMC7113115, PMC9134917. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/RBBP4/immunohistochemistry-ihc-guide.