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We validate the specificity of these antibodies to Rbfox3 by testing them on tissues known to express Rbfox3 positively and negatively. Browse below to find the Rbfox3 antibody that suites your experiment. We have 7 of these antibodies and many publications and validation images.
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Facts about RNA binding protein fox-1 homolog 3.
FLJ56884; FLJ58356; Fox-1 homolog C; FOX3; FOX-3; hexaribonucleotide binding protein 3; HRNBP3; NeuN flow cytometry; NeuN; neuronal nuclei; RBFOX3; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog (C. elegans) 3
|Location:||11 E2|11 83.22 cM|
Widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord (at protein level). Not expressed in all neuronal cells within a region, in cerebellum, expression is absent in Purkinje cells (at protein level). Expressed in the retina in the ganglion cells and some cells in the inner nuclear layer, but absent from the photoreceptor cells and most cells in the inner nuclear layer (at protein level).
RBFOX3 is a gene and protein target that may also be known as FOX3, HRNBP3, NeuN, or RNA binding protein. The antigen has 33.9 kilogramaltons (in mass). Although its neuronal genesis remains undetermined, the antigen has been identified in a variety animal species such as canines and monkeys.
Boster Bio sells RBFOX3 antibodies. Boster Bio is a biotechnology firm that specializes in the development and distribution of ELISA kits for picogram sensitivities kits and research antibodies. Boster's antibody repertoire includes over 12,000 antibodies with multiple formats and immunogens. Every antibody is rigorously validated against WB, IHC, and Flow applications. Tests on 250 untransfected cells and tissues is performed for each antibody.
The RBFOX3 gene encodes a member of the FOX RNA-binding family, that plays a significant part in neuronal development and regulates adult brain function. The gene is widely expressed in the central nervous system, and is an excellent marker for post-mitotic neuronal activity. It also regulates the alternative splicing of pre-mRNA and regulates neuronal development and function.
Boster Bio's RBFOX3 antigen is part of Picoband(tm). It interacts with humans, rats and Mouse. The antigen can be stored at 4°C up to one month. The buffer containing the antibody is made up of Trehalose, NaCl and Na2HPO4 and can be stored for up to a month.
RBFOX3 (Rbfox-33) proteins are involved in autoregulation, alternative splicing regulation, and have a wide variety of targets. However, their exact purpose is still unknown. Known to regulate pre-mRNA alternative Splicing, RBFOX3/NeuN helps regulate neuronal differentiation, and is implicated in a wide range of neurological disorders. Dysregulation of the protein is believed to be a contributor in a myriad of neurological disorders, including epilepsy, Parkinson's disease and Alzheimer's.
This protein is present in neuronal cells. It comes in two forms: cytoplasmic and nuclear. Neurons express NeuN and mAbA60 is a reliable neuronal biomarker. Doublecortin inhibition is only the one method to detect NeuN immunoreactivity. However, NeuN immunoreactivity is not detectable in other neuronal types.
There are numerous suppliers of NeuN antibodies. These antibodies recognize NeuN in RBFOX3 the neuronal marker. The antibody binds to an epitope located in the protein's N-terminus at amino acids one to twenty. The epitope is located in exon 5 of the gene ENSMUST00000017576. The antibody also recognizes exon 6 and 8, two important RNA binding sites.
The RBFOX3 gene encodes NeuN, neuronal biomarker. The RBFOX family is comprised of three members, all of which participate in alternative RNA transcription. The NeuN protein is a single RNA recognition motif and RNA binding domain which is conserved across mammals. It also plays a part in alternative splicing of its members and in autoregulation.
Utilizing RNA-Seq, gene expression was measured in the cerebral cortex and hippocampus of mice. The RNA was isolated from the brain using a NucleoSpin(r) miRNA kit. The ND1000 spectrophotometer was used to measure the RNA. After the RNA was extracted library building was done using the SureSelect Strand Specific RNA Library Prep Kit II.
After single blots had confirmed target protein staining, double fluorescent western blots could be performed. Equal amounts of protein samples were separated by SDS-PAGE gels (7.5 percent) and then transferred onto the membranes with nitrocellulose. After blocking, the membranes were incubated with rabbit anti-NeuN antibody and Millipore A60 aswell in Syn I, a mouse monoclonal antibody.
In this study, the role of dysfunctional RBFOX3 is discussed in relation to various neurodevelopmental disorders. The expression of Rbfox3 in various regions of the brain could vary during development. Mice that lack RBFOX3 show reduced neurogenesis, cold hyperalgesia and impaired cognition. mice with Rbfox3-/ showed more excitatory synapses.
The brain is known to have the highest levels of transcriptional activity. For the brain to function and to maintain homeostasis, rapid changes in gene expression are essential. The splicing factor Rbfox3 was recently identified as a possible target for anti-NeuN antibodies. Rbfox3 is part of the Fox-Family. It recognizes a unique motif within introns. Although its function in disease is not known but it is believed the altered location of Rbfox3 causes downregulation in neuronal gene expression.
The decrease in the expression of synaptic proteins is linked with a decrease in synaptic transmission within the region of the device. The loss of dendrites and spines is most likely to be a reason. It is still unclear whether this gene could be used as a biomarker to detect neurological disorders. However its expression could be useful for the identification of mutations that cause disease in a patient's condition.
The subcellular location of NeuN can be used to detect neurodegenerative disorders and HIV infection. The study examined brain tissue sections of participants from the NNTC, a prospective longitudinal cohort that collects brain tissue from four clinical locations in the United States. People who donate their brain tissue for research are followed for a long time and are evaluated clinically and are subjected to neuropsychological testing.
Although it's not clear what role RBFOX3 plays in fear the genes are associated with neuronal differentiation. The function of RBFOX3 is critical for normal hippocampal development and brain function in the brain. A malfunctioning RBFOX3 can cause synaptic transmission to become affected and short- and long-term plasticity impairment. The result is an impairment in cognition and memory.
It is interesting to consider that the proteins involved in this process are Mbp1 and Plp1. In particular, Mbp is connected to the construction of axonal myelin. The increased levels of these proteins could be a sign of the need to repair myelin. It is also linked to inflammation, microglial activation and many other interesting things. This suggests that this protein may be involved in the regulation of microglia and complement system.
If you are interested in studying NeuN expression in neuronal cell and tissue, an Anti-NeuN antibody made with Boster Bio's RBfox3 marker is a great choice. NeuN is neuronal nucleic antigen that is a popular biomarker. The expression of mRNA is analyzed with RT-PCR. The antibody recognizes Synapsin 1 which is the largest protein found in neurons.
The sequence of 21 amino acids in the protein was used to determine the target of an anti NeuN antibody. The antibody is detected using immunoprecipitation as well as mass spectrometry. This technique is quicker than other methods used to analyze NeuN expression. It is applicable to a wide range of cells that include neurons as well in other proteins.
A RNA binding protein is essential for CLIP to function properly. The anti-NeuN antibody binds Rbfox3 to a complex of bound RNAs. This antibody also has a strong IP activity against Rbfox3. Neuronally enhanced This is the RBFOX3 marker has been identified. Many other immunogenic proteins are also target proteins. High-throughput sequencing techniques can determine the target protein in vivo with CLIP or other methods.
Transient transfection of the Rbfox3 protein fusion into N2A cells was performed. The transfection was performed as described above, however, the Rbfox3 variants were used as positive control for treatment with emetine. The RT-PCR technique was employed to determine alternative splicing of mRNAs produced by endogenous mRNAs. Anti-actin was also employed as an indicator of loading.
RBFOX3 is part of the Fox family of alternative splicing regulators. Researchers can determine Rbfox3 expression in neurons using the RBFOX3 marker. Both the lower and upper bands of gel have Rbfox3 peptides. However the Rbfox3 variants do not have any effect on CaV1.2 exons 9* and 33.
Anti-NeuN antibody uses RBFOX3 marker made by BoSTER Bio to visualize NeuN expression in neuronal cells. The protein was created in bacterial cells by IPTG induction. The antibody recognizes R3H, which is found in the R3Hdomain. The protein was normalized against b-actin which is a protein of the same size as the target protein. The R3Hdm2 spectrum was calculated relative to RBFOX3 expression within the Thymus.
*More publications can be found for each product on its corresponding product page