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- Table of Contents
RPL10A / Large ribosomal subunit protein uL1 · Western blot design guide
Design a Western Blot for RPL10A
Source-linked RPL10A Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-RPL10A WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
RPL10A Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~24.8 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Appendix (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked RPL10A Western Blot Protocol Options
The A08606 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | extracts of various cells, (catalog A08606) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A08606; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected RPL10A Western Blot Band Size?
RPL10A is predicted at 24.8 kDa; annotated modifications could affect migration, but no empirical band or modification-dependent shift is established.
| Band near 24.8 kDa | consistent with the predicted RPL10A size; confirm identity with antibody controls |
| Band in cytoplasmic lysate | consistent with the reported cytoplasmic location |
| Little or no band in a cytoplasm-depleted fraction | consistent with cytoplasmic localization |
| Additional nearby band | identity is uncertain; annotated modifications have no demonstrated migration effect |
💡Expected RPL10A appearanceRPL10A has a predicted mass of 24.8 kDa, but no empirical band size is supplied; verify a band near that size with antibody specificity controls.
| Predicted molecular mass | places the unmodified protein near 24.8 kDa |
| N-acetylserine at residue 2 | is annotated, but no apparent size change is established |
| Phosphotyrosine at residue 11 | is annotated, but no resolvable band shift is established |
| N6-acetyllysine at residues 91, 106 and 118 | is annotated, but no apparent size change is established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | poor recovery of cytoplasmic protein or inadequate detection | check cytoplasmic protein recovery and a positive lysate control |
| Band higher than expected | band identity or migration is unestablished | check antibody specificity and sample denaturation |
| Band lower than expected | possible proteolysis or nonspecific binding | compare a fresh lysate and an independent antibody |
| Multiple bands | additional bands cannot be assigned to the single listed isoform or its modifications | check specificity with an RPL10A depletion control |
| Weak or no signal | low cytoplasmic protein recovery or insufficient detection | verify loading and use a positive lysate control |
| Fragments below expected size | possible sample degradation | prepare fresh lysate with protease inhibitors |
Experiment evidence
Western blot images showing RPL10A band
A08606 · RPL10A
M08606-1 · RPL10A
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for RPL10A Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for RPL10A in Western blot, you can use appendix tissue, which HPA rates High.
Positive control: Appendix (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA reports no detection in adipose tissue, but confirm its suitability as a negative by Western blot.
HPA tissue expression evidence for RPL10A
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Cerebellum | Purkinje cells | High | Protein (IHC) | HPA → |
| Esophagus | squamous epithelial cells | High | Protein (IHC) | HPA → |
| Fallopian tube | glandular cells | High | Protein (IHC) | HPA → |
| Oral mucosa | squamous epithelial cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Parathyroid gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Low | Protein (IHC) | HPA → |
| Lymph node | non-germinal center cells | Low | Protein (IHC) | HPA → |
| Ovary | follicle cells | Low | Protein (IHC) | HPA → |
Section 3
Advanced RPL10A Western Blot Tips
Deeper troubleshooting and optimisation questions for RPL10A, answered from its protein features.
How should RPL10A band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could RPL10A isoforms explain multiple bands?
Isoforms · Only one isoform is listed, with no alternative sequence. The supplied features therefore do not support assigning multiple bands to different RPL10A isoforms.
Which RPL10A acetylation sites matter when interpreting bands?
PTM · UniProt lists N-acetylserine at position 2 and N6-acetyllysine at positions 91, 106, and 118; position 118 is marked alternate. These are UniProt canonical-sequence coordinates and may differ from antibody or paper numbering. Their presence does not establish a visible band shift.
UniProt lists phosphotyrosine at position 11 of its canonical sequence. Check the numbering convention when comparing antibody or paper labels. This feature alone does not show whether phosphorylation changes the Western blot band.
UniProt lists phosphotyrosine at position 11 of its canonical sequence. Check the numbering convention when comparing antibody or paper labels. This feature alone does not show whether phosphorylation changes the Western blot band.
Does this guide establish induction of RPL10A?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for RPL10A?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A08606 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should RPL10A Western blot signal be quantified?
Quantitation · Measure the same assigned RPL10A band consistently across samples and record how any additional bands were handled. RPL10A is a cytoplasmic component of the large ribosomal subunit; a change in its band signal alone does not establish a change in ribosomal activity.
Should RPL10A run exactly at its predicted mass?
Interpretation · The predicted mass is 24.8 kDa, but no observed Western blot band is supplied. Use 24.8 kDa as a reference, not a guaranteed migration position; the listed modifications alone do not establish a visible shift.
Compare them with the 24.8 kDa predicted mass, but do not assign them to isoforms or specific modifications from migration alone. The supplied features list one isoform and several modified residues, without an observed band or evidence that any modification produces a distinct band.
Compare them with the 24.8 kDa predicted mass, but do not assign them to isoforms or specific modifications from migration alone. The supplied features list one isoform and several modified residues, without an observed band or evidence that any modification produces a distinct band.
Boster reagents
RPL10A Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-60S ribosomal protein L10a RPL10A Antibody
Cat # A08606
Real WB data
Anti-RPL10A Rabbit Monoclonal Antibody
Cat # M08606-1
The catalog reports two anti-RPL10A antibodies for Western blotting, both with Human, Mouse, and Rat reactivity and WB images. The supplied captions identify HeLa lysate for M08606-1 and unspecified cell extracts for A08606; they do not establish WB performance in every listed species.
Which to pick: For HeLa lysate, M08606-1 has a directly matching WB image. A08606 also has a WB image, but its caption only specifies various cell extracts. Both list Human, Mouse, and Rat reactivity; choose based on the sample context documented for your experiment.
Source: BosterBio RPL10A gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P62906.
- Human Protein Atlas. RPL10A tissue expression.
- PMC9485161 — target-verified WB comparison
- PMC11787193 — target-verified WB comparison
- PMC12623983 — target-verified WB comparison