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- Table of Contents
RPLP1 / Large ribosomal subunit protein P1 · Western blot design guide
Design a Western Blot for RPLP1
Source-linked RPLP1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-RPLP1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
RPLP1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~11.5 kDa | |
| Gel | 15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked RPLP1 Western Blot Protocol Options
The A07117 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | extracts of various cell lines, (catalog A07117) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | Blocking buffer: 3% nonfat dry milk in TBST (catalog A07117) |
| Primary antibody | A07117 · 1:1000 (catalog A07117) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Goat Anti-Rabbit IgG, 1:10000 (catalog A07117) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL (catalog A07117) |
Section 2
What Is the Expected RPLP1 Western Blot Band Size?
RPLP1 is predicted at 11.5 kDa; isoforms and modifications could affect migration, but no empirical band size or specific shift is established.
| Band near 11.5 kDa | consistent with the predicted RPLP1 size; confirm identity with antibody controls |
| Two bands near the expected region | could reflect isoforms 1 and 2 if their migration differs |
| Close doublet | could reflect phosphorylation at Ser101; the migration effect is unestablished |
| Higher band | could reflect Ubl conjugation; band identity requires confirmation |
💡Expected RPLP1 appearanceRPLP1 has a predicted mass of 11.5 kDa, but no empirical band size is supplied; isoforms and modifications do not establish a specific migration pattern, so confirm band identity with appropriate controls.
| Predicted RPLP1 mass | places the unmodified sequence near 11.5 kDa |
| Isoform 1 | may migrate differently from isoform 2; its mass is unspecified |
| Isoform 2 | may migrate differently from isoform 1; its mass is unspecified |
| N-acetylalanine at residue 2 | is documented, but a visible size shift is unestablished |
| Phosphoserine at residue 101 | may alter mobility, but a visible shift is unestablished |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | possible Ubl conjugation or nonspecific binding | check specificity with RPLP1 knockdown and compare treatment conditions |
| Band lower than expected | possible degradation or nonspecific binding | prepare fresh lysate with protease inhibitors and check RPLP1 knockdown |
| Multiple bands | possible isoforms, phosphorylation states, or nonspecific binding | compare knockdown lanes and test phosphatase treatment |
| Weak or no signal | low RPLP1 recovery or inadequate antibody detection | check loading and transfer with controls and optimize antibody conditions |
| Fragments below expected size | possible proteolysis during sample preparation | use fresh lysate with protease inhibitors and check whether fragments track with RPLP1 knockdown |
Experiment evidence
Western blot images showing RPLP1 band
A07117 · RPLP1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for RPLP1 Western blot
🧪For positive controls for RPLP1 in Western blot, you can use a validated RPLP1-positive sample, but the supplied HPA evidence identifies none.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA expression data are unavailable, so tissue-based controls cannot be selected from this evidence.
HPA tissue expression evidence for RPLP1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced RPLP1 Western Blot Tips
Deeper troubleshooting and optimisation questions for RPLP1, answered from its protein features.
How should RPLP1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could RPLP1 isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2; isoform 2 lacks canonical residues 25–49. They may differ in size, but no band positions are supplied. Check whether the antibody epitope includes residues 25–49 before interpreting a missing or additional band.
Decide whether the measurement should include both isoforms. Isoform 2 lacks canonical residues 25–49, so an antibody recognizing that segment may not measure both equally. Report which band or bands were quantified.
Decide whether the measurement should include both isoforms. Isoform 2 lacks canonical residues 25–49, so an antibody recognizing that segment may not measure both equally. Report which band or bands were quantified.
Which RPLP1 phosphorylation site matters for interpreting bands?
PTM · UniProt lists phosphoserine at canonical position 101. Consider phosphorylation when investigating band differences, but the feature alone does not show that it causes a visible shift. State the numbering convention when comparing this site with antibody or paper annotations.
Yes. UniProt lists N-acetylalanine at canonical position 2. Keep that coordinate when recording the modification; it does not, by itself, establish a distinct Western-blot band.
Yes. UniProt lists N-acetylalanine at canonical position 2. Keep that coordinate when recording the modification; it does not, by itself, establish a distinct Western-blot band.
Does this guide establish induction of RPLP1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for RPLP1 Western blot?
Transfer · RPLP1 is predicted to be 11.5 kDa, so check that the chosen transfer conditions retain a small protein on the membrane. The supplied features do not specify a transfer method or settings.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A07117 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should RPLP1 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Does RPLP1 have a documented band shift from 11.5 kDa?
Interpretation · No observed band is supplied. The 11.5 kDa value is predicted, and the listed modifications do not establish a visible shift. Compare any measured band with the predicted mass without assigning a cause from these features alone.
First compare them with the predicted 11.5 kDa protein and the isoform 2 deletion of canonical residues 25–49. RPLP1 also forms a heterodimer with RPLP2 at the ribosomal stalk, but that feature does not establish a Western-blot band. The listed modifications likewise do not identify an unexpected band on their own.
First compare them with the predicted 11.5 kDa protein and the isoform 2 deletion of canonical residues 25–49. RPLP1 also forms a heterodimer with RPLP2 at the ribosomal stalk, but that feature does not establish a Western-blot band. The listed modifications likewise do not identify an unexpected band on their own.
Boster reagents
RPLP1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-RPLP1 Antibody
Cat # A07117
the supplier A07117 is an anti-RPLP1 antibody listed for human, mouse, and rat. Its Western blot image uses extracts from various cell lines at 1:1000 dilution with 25 µg per lane; the supplied caption does not identify the cell lines or their species.
Which to pick: A07117 is the only listed option and has a Western blot image. Check its listed human, mouse, and rat reactivity against your sample; the image caption does not establish which species were tested.
Source: BosterBio RPLP1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P05386.
- Human Protein Atlas. RPLP1 tissue expression.
- PMC4069005 — target-verified WB comparison
- PMC9917350 — target-verified WB comparison