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- Table of Contents
RPS10 / Small ribosomal subunit protein eS10 · Western blot design guide
Design a Western Blot for RPS10
Source-linked RPS10 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-RPS10 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
RPS10 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~18.9 kDa | |
| Gel | 15% (standard starting point) | |
| Positive control | Breast (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Methylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked RPS10 Western Blot Protocol Options
The A06898 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | various lysates, (catalog A06898) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | Blocking buffer: 3% nonfat dry milk in TBST (catalog A06898) |
| Primary antibody | A06898 · 1:400 (catalog A06898) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Goat Anti-Rabbit IgG, 1:10000 (catalog A06898) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL (catalog A06898) |
Section 2
What Is the Expected RPS10 Western Blot Band Size?
RPS10 is predicted at 18.9 kDa; its listed modifications could affect mass, but no empirical migration or distinct band pattern is demonstrated.
| Single band near 18.9 kDa | Consistent with the predicted size of RPS10; confirm identity with antibody controls |
| Band near 18.9 kDa in cytoplasmic fraction | Consistent with cytoplasmic RPS10 |
| Band near 18.9 kDa in nucleolar fraction | Consistent with nucleolar RPS10 |
| Weak or absent nucleolar band | Could reflect reduced nucleolar localization, which requires methylation |
💡Expected RPS10 appearanceRPS10 has a predicted mass of 18.9 kDa, but no empirical band size is supplied; use band identity controls to assess any signal near that size.
| UniProt predicted mass | Places unmodified RPS10 near 18.9 kDa |
| Phosphotyrosine at residue 12 | Adds a phosphate; a visible migration change is not established |
| Phosphoserine at residue 146 | Adds a phosphate; a visible migration change is not established |
| Omega-N-methylarginine at residue 153 | Adds a methyl group; a visible migration change is not established |
| Symmetric dimethylarginine at residues 158 and 160 | Adds methyl groups; a visible migration change is not established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | RPS10 localization across cytoplasm and nucleolus may affect recovery | Check loading and compare cytoplasmic and nuclear fractions |
| Band higher than expected | The listed modifications do not establish a large size shift | Verify antibody specificity and compare with a validated RPS10 control |
| Band lower than expected | No signal peptide or propeptide cleavage is listed | Check band identity with an independent RPS10 antibody |
| Multiple bands | Modification sites are listed, but distinct migrating forms are not established | Validate each band with an independent antibody or RPS10 depletion |
| Weak or no signal | Subcellular distribution may reduce RPS10 abundance in the sampled fraction | Check loading and sample cytoplasmic and nucleolar fractions |
Experiment evidence
Western blot images showing RPS10 band
A06898 · RPS10
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for RPS10 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for RPS10 in Western blot, you can use breast tissue, which has high HPA expression.
Positive control: Breast (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside.
⚠️Feasibility: Whole-cell lysate is suitable for this intracellular protein; HPA reports no detection in adipose tissue.
HPA tissue expression evidence for RPS10
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Breast | glandular cells | High | Protein (IHC) | HPA → |
| Duodenum | glandular cells | High | Protein (IHC) | HPA → |
| Esophagus | squamous epithelial cells | High | Protein (IHC) | HPA → |
| Pancreas | exocrine glandular cells | High | Protein (IHC) | HPA → |
| Small intestine | glandular cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Ovary | follicle cells | Not detected | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | smooth muscle cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced RPS10 Western Blot Tips
Deeper troubleshooting and optimisation questions for RPS10, answered from its protein features.
Where should the main RPS10 band appear?
Band shift · The calculated mass is 18.9 kDa. No observed band position is supplied, so use 18.9 kDa as a reference, not a confirmed apparent mass.
Could an RPS10 isoform explain a second band?
Isoforms · The supplied entry lists one isoform and no alternative sequence. These features do not support assigning a second band to a listed RPS10 isoform.
Which RPS10 modifications should I consider when interpreting bands?
PTM · UniProt lists phosphotyrosine at position 12, phosphoserine at 146, omega-N-methylarginine at 153, and symmetric dimethylarginine at 158 and 160. These are UniProt sequence coordinates; antibody or paper numbering may differ. Their presence alone does not establish a visible shift.
UniProt lists phosphorylation at Tyr12 and Ser146. Neither site establishes that a shift will be visible, and no observed band position is provided. Treat a shifted band as unresolved without evidence connecting it to RPS10 phosphorylation.
UniProt lists phosphorylation at Tyr12 and Ser146. Neither site establishes that a shift will be visible, and no observed band position is provided. Treat a shifted band as unresolved without evidence connecting it to RPS10 phosphorylation.
Does this guide establish induction of RPS10?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for RPS10?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A06898 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should I quantify RPS10 across cellular fractions?
Quantitation · RPS10 is reported in both cytoplasm and nucleolus. Quantify matched fractions separately and keep sample preparation consistent; a change in one fraction can reflect altered localization rather than a change in total RPS10.
Does methylation affect where RPS10 is found?
Interpretation · The supplied features state that methylation is required for RPS10 localization in the granular component of the nucleolus. RPS10 is also reported in the cytoplasm, so compare the same cellular fraction across samples when assessing abundance.
RPS10 is a component of the small ribosomal subunit, and its methylated form interacts with NPM1. Those associations alone do not identify an unexpected Western blot band; avoid assigning it to an RPS10 complex from size alone.
RPS10 is a component of the small ribosomal subunit, and its methylated form interacts with NPM1. Those associations alone do not identify an unexpected Western blot band; avoid assigning it to an RPS10 complex from size alone.
Boster reagents
RPS10 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-40S ribosomal protein S10 RPS10 Antibody
Cat # A06898
A06898 is an anti-RPS10 antibody listed for Human, Mouse, and Rat reactivity. Its WB image uses various lysates at 1:400 and 25 µg per lane. The caption does not identify the lysates, and no publication evidence is supplied.
Which to pick: A06898 is the only listed antibody and has a WB image. Consider its stated reactivity and the reported WB conditions for your experiment; the image caption does not establish which species were tested.
Source: BosterBio RPS10 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P46783.
- Human Protein Atlas. RPS10 tissue expression.
- PMC8198291 — target-verified WB comparison
- PMC8565352 — target-verified WB comparison