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- Table of Contents
RPS11 / Small ribosomal subunit protein uS17 · Western blot design guide
Design a Western Blot for RPS11
Source-linked RPS11 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-RPS11 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
RPS11 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~18.4 kDa | |
| Gel | 15% (standard starting point) | |
| Positive control | Small intestine (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked RPS11 Western Blot Protocol Options
The A07622S11 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | lysates from HeLa and Jurkat cells, (catalog A07622S11) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | the synthesized peptide (catalog A07622S11) |
| Primary antibody | A07622S11; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected RPS11 Western Blot Band Size?
RPS11 is predicted at 18.4 kDa; its annotated modifications could affect migration, but no empirical band or demonstrated shift is supplied.
| Single band near 18.4 kDa | Consistent with the predicted size of RPS11; confirm identity with antibody controls |
| Band in a cytoplasmic fraction | Consistent with the annotated cytoplasmic location |
| Band in a nuclear or nucleolar fraction | Consistent with the annotated nuclear and nucleolar location |
| Bands in both cytoplasmic and nuclear fractions | Consistent with the two annotated cellular locations |
💡Expected RPS11 appearanceRPS11 has a predicted mass of 18.4 kDa, but no empirical band size is supplied; a band near that size needs confirmation with antibody controls, and annotated modifications do not establish a visible shift.
| Predicted mass | Places the expected unmodified protein near 18.4 kDa |
| N-acetylalanine at residue 2 | Adds a small modification; a visible migration change is not established |
| N6-acetyllysine at residues 38, 45 and 58 | Adds modifications; their effect on apparent band size is not established |
| Phosphoserine at residues 67 and 110 | May affect migration if present, but no band shift is demonstrated |
| Citrulline at residue 22 and methylarginine at residue 69 | Mark modified residues without an established band-size effect |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Ribosome-associated or nucleolar RPS11 may be poorly recovered | Check extraction and loading, then compare cellular fractions |
| Band higher than expected | The listed modifications may affect migration, but no shift is established | Confirm identity with peptide competition or RPS11 depletion |
| Band lower than expected | No signal-peptide or propeptide cleavage is annotated | Confirm identity with RPS11 depletion and check sample integrity |
| Multiple bands | Modified RPS11 is annotated, but distinct migrating forms are unproven | Use peptide competition or RPS11 depletion to identify specific bands |
| Weak or no signal | Recovery of ribosome-associated RPS11 may be limited | Check loading, extraction and antibody performance |
Experiment evidence
Western blot images showing RPS11 band
A07622S11 · RPS11
A07622S11 · RPS11
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for RPS11 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for RPS11 in Western blot, you can use small intestine tissue, the highest-scoring HPA positive sample.
Positive control: Small intestine (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: RPS11 is intracellular, so tissue lysates are suitable; verify the HPA-negative adipose tissue result by Western blot.
HPA tissue expression evidence for RPS11
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Small intestine | glandular cells | High | Protein (IHC) | HPA → |
| Appendix | glandular cells | Medium | Protein (IHC) | HPA → |
| Cervix | squamous epithelial cells | Medium | Protein (IHC) | HPA → |
| Duodenum | glandular cells | Medium | Protein (IHC) | HPA → |
| Epididymis | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced RPS11 Western Blot Tips
Deeper troubleshooting and optimisation questions for RPS11, answered from its protein features.
How should RPS11 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could RPS11 isoforms explain multiple bands?
Isoforms · Only one isoform is listed, with no alternative sequence. These features do not support assigning additional bands to RPS11 isoforms.
Which RPS11 modifications matter when interpreting bands?
PTM · UniProt lists N-acetylalanine at 2, citrulline at 22, N6-acetyllysine at 38, 45 and 58, phosphoserine at 67 and 110, and omega-N-methylarginine at 69. These are UniProt coordinates; antibody or paper numbering may differ. Their presence does not establish a visible band shift.
Does this guide establish induction of RPS11?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for RPS11 Western blot?
Transfer · RPS11 is predicted to be 18.4 kDa. Use transfer conditions suited to retaining a small protein, such as a 0.2 µm membrane, and check transfer with a total-protein stain.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A07622S11 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should RPS11 bands be quantified across samples?
Quantitation · RPS11 is found in the cytoplasm and nucleolus and is part of the small ribosomal subunit and SSU processome. Compare the same sample fraction across lanes and normalize the RPS11 signal to total protein in that fraction.
Should RPS11 migrate at exactly 18.4 kDa?
Interpretation · 18.4 kDa is the predicted mass; no observed band position is supplied. Compare the signal with a molecular weight marker, but do not infer a visible shift from the listed modifications alone.
Check each band's size against the predicted 18.4 kDa mass and confirm its identity before assigning it to RPS11. One isoform is listed, and the modification sites alone do not explain an extra band or a particular mass difference.
Check each band's size against the predicted 18.4 kDa mass and confirm its identity before assigning it to RPS11. One isoform is listed, and the modification sites alone do not explain an extra band or a particular mass difference.
Boster reagents
RPS11 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Ribosomal Protein S11 RPS11 Antibody
Cat # A07622S11
The catalog reports A07622S11 for RPS11 Western blotting, with stated human, mouse, and rat reactivity. Its WB image shows HeLa and Jurkat lysates and a peptide-blocked lane; the supplied image does not show mouse or rat samples.
Which to pick: A07622S11 is the only listed option. It has a WB image using HeLa and Jurkat lysates. For mouse or rat samples, reactivity is listed, but no corresponding WB sample is shown.
Source: BosterBio RPS11 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P62280.
- Human Protein Atlas. RPS11 tissue expression.
- PMC7646677 — target-verified WB comparison
- PMC11922711 — target-verified WB comparison
- PMC7210141 — target-verified WB comparison