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- Table of Contents
2 Citations 10 Q&As
12 Q&As
Facts about Pulmonary surfactant-associated protein D.
May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Human | |
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Gene Name: | SFTPD |
Uniprot: | P35247 |
Entrez: | 6441 |
Belongs to: |
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SFTPD family |
COLEC7collectin-7; Collectin 7; Collectin-7; Lung surfactant protein D; PSPD; PSP-D; SFTP4; SFTP4pulmonary surfactant-associated protein D; SFTPD; SPD; SP-D; SP-Dpulmonary surfactant apoprotein; surfactant protein D; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4
Mass (kDA):
37.728 kDA
Human | |
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Location: | 10q22.3 |
Sequence: | 10; NC_000010.11 (79937740..79982236, complement) |
Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).
Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
Boster Bio has just developed the SFTPD marker. It is a new method to determine S-Factor and F-Test in human samples. This new method can be used by all scientists across the globe. Read more to discover the advantages and the background of this biomarker. You will also find the most recent information and applications.
The primary role of SP-D is the lung. It is therefore beneficial in the detection of lung inflammation. The SFTPD marker offers many advantages in lung disease research. We will explore a few of these advantages. SP-D is essential to lung function. SP-D is vital for lung function. It regulates oxidant production, inflammation in alveolar macrophages , and clearing of cells that have died.
Serum levels of SFTPD differ between individuals and the aa11-C allele showing a lower percentage of SP-D levels in serum than its counterpart. This is due to an SNP located in the N-terminal region that influences oligomerization. Therefore, aa11-C homozygotes lack the highest-molecular-weight forms of the protein.
The activation of SPD blocks the migration of macrophages from interstitium pulmonary to alveoli as well as BALF. In a mouse study lung tissues were stained with an anti-macrophage antibody called MAC-3. After an indirect lung injury, Sftpd/ mice showed a twofold increase in positive MAC-3-positive cells. Wild-type mice didn't exhibit this increase.
If lung damage is caused by an inhaled pathogen, SP-D levels increase significantly in comparison to wild-type mice. The serum concentrations of IL-6 in mice without SFTPD were nearly four times higher than those in wild-type mice. The increased pulmonary inflammation observed in Sftpdmice could be caused by systemic inflammation. It is therefore crucial to identify the source of inflammation throughout the body that can cause elevated levels of IL-6 in Sftpd-/ mice.
The SFTPD gene promoter is activated by calcineurin which is an endogenous protein that belongs to the NFAT pathway in lung epithelial cells. Additionally the NFATs NFATc3 and TTF-1 interact with the promoter and activate transcription. SP-D is expressed in response to the activation of these proteins. The clinical applications of the SFTPD marker include lung cancer diagnosis as well as prevention, early treatment and early treatment.
Patients with ARDS have higher levels of SFTPD expression. This is due to the production pro-SFTPB. These cells possess anti-inflammatory as well as antioxidant properties. Patients suffering from lung scarring could have higher levels of pro-SFTPB. This could be due in part to the increased permeability in the lung tissues. Therefore, the SFTPD gene is a possible marker of the EGFR-TKI's efficacy.
The SFTPD gene is located at 10q21-24. It is composed of three genes including SFTPA1, and SFTPD. The study was intended to assess linkage disequilibrium between these genes as well as to study the connection between genetic variability, susceptibility to community acquired pneumonia, and genetic variability. The blood also contained the SP-D marker. It was associated with various outcomes of CAP as well as its severity.
Serum levels of pro-SFTPB in HIV-infected individuals were significantly higher than in non-infected HIV patients. After taking into account age race, and smoking status, the SFTPD levels in HIV-infected persons were significantly higher than in people who are not HIV-infected. Additionally that pro-SFTPB levels were more elevated for whites than for blacks. The relationship between pro-SFTPB levels and SFTPD level was less pronounced.
A case-control study was conducted in order to determine susceptibility and the outcome of CAP. A prospective cohort of patients with CAP was also included in the study. The demographics of patients were recorded in the Additional File 1. Serum protein levels were assessed using an ELISA kit purchased from Antibodyshop (r) in Gentoft, Denmark. Further studies are required to confirm the SFTPD marker in the larger sample.
Boster Bio's high-quality lysate with protein-A/G affinity beads is a convenient and highly efficient method for antibody purification. The beads are coated with highly specific secondary antibodies that are useful to purify specific types of antibodies. In addition to high-quality lysate, Boster Bio's products also are highly compatibility with other substances.
To use Boster Bio's high-quality lysate with protein-A/G affinity beads, simply prepare a sample in a microplate-compatible lysis buffer. Add 70-100mL of beads to each sample and keep it on ice. To avoid contamination, use a pipette with an edge cut 5 millimeters from the top.
The yield of protein produced from the day one experiment is contingent on the volume of lysis buffer used as well as the type of cells and the growth pattern of the sample. For 1x1011-1x1011 cells an average of 12-20mg of protein is typical. You can determine the quality of the product after coupling by running 10% (vol/vol), SDS-PAGE. If the antibody coated with covalents is stable the heavy chain will stay connected to the beads after boiling two times LSB.
PMID: 1339284 by Lu J., et al. Purification, characterization and cDNA cloning of human lung surfactant protein D.
PMID: 8428971 by Crouch E., et al. Genomic organization of human surfactant protein D (SP-D). SP-D is encoded on chromosome 10q22.2-23.1.
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