Human SP-D / Surfactant protein D ELISA Kit PicoKine®

Product Name

Human SP-D / Surfactant protein D ELISA Kit PicoKine®

SKU/Catalog Number

EK1171

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

SFTPD (surfactant protein D; SP-D) is a collectin that contributes to pulmonary innate defense by binding microbial ligands (e.g., LPS) and can engage pattern-recognition receptor modules (e.g., reported interactions with TLR complexes in curated pathway resources). SP-D is also widely studied as a circulating biomarker of alveolar-capillary barrier injury in acute/chronic lung disease contexts (clinical interpretation depends on cohort and assay). Assay context: quantitative human SFTPD ELISA for cell culture supernatants, cell lysates, serum, and plasma (heparin/EDTA); sensitivity 20 pg/mL; can be interpreted alongside anti-inflammatory cytokines such as IL-10 when profiling inflammation–resolution balance (putative co-panel).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human SP-D / Surfactant protein D ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1171)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: A21-F375

Capture/Detection Antibodies

The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.

Sensitivity

<20 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Standard Dilution Instructions

See datasheet of EK1171 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1171 is reactive to SFTPD in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1171 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of SFTPD

Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Kit Components

Catalog Number	Description	Quantity
EK1171-CAP	Anti-Human SFTPD Pre-coated 96-well strip microplate	1
EK1171-ST	Human SFTPD Standard	2 vials, 40 ng/tube
EK1171-DA	Human SFTPD Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)	0	0.625	1.25	2.5	5	10	20	40
O.D.	0.09	0.138	0.179	0.286	0.497	0.766	1.456	2.084

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect SFTPD, Dilution ratio of 1:1, concentration in serum and plasma is around 2.6 ng/ml..

Some articles we found to cite concentrations of SFTPD in samples: 25488319 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human SP-D / Surfactant protein D ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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