This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Homeobox protein SIX3.
Acts as a direct upstream activator of SHH expression in the rostral diencephalon ventral midline and that subsequently SHH maintains its expression. Additionally, Six3 activity is required for the formation of the telencephalon.
Human | |
---|---|
Gene Name: | SIX3 |
Uniprot: | O95343 |
Entrez: | 6496 |
Belongs to: |
---|
SIX/Sine oculis homeobox family |
holoprosencephaly 2, alobar or semilobar; homeobox protein SIX3; HPE2; sine oculis homeobox homolog 3 (Drosophila); Sine oculis homeobox homolog 3; SIX homeobox 3
Mass (kDA):
35.487 kDA
Human | |
---|---|
Location: | 2p21 |
Sequence: | 2; NC_000002.12 (44941702..44946071) |
Nucleus.
In this article, you'll discover the advantages of the SIX3 Marker for QRT-PCR. Also, you will learn about ISH primers for lncRNAs GLIS2-AS1 or the PVT1 as well as the SIX3 marker used for ISH. Additionally, you will learn how to perform qRT-PCR on a Step-One Plus Detection System.
The StepOnePlus(tm) Real-Time PCR System has a user-friendly LCD touchscreen that has a graphic user interface. The software is easy to use and includes wizards for the protocol. The system is calibrated in the factory to give accurate optical and thermal results. This system can be used with a separate instrument or computer.
Utilizing a probe-based RTPCR protocol and SYBR Green for the template, the qRTPCR was done. The primers are compatible in size, with each 132 bp. In both cases, a negative control (a non-template control) was added to the reaction tubes. The qRT-PCR assay was carried out as directed by the instrument manufacturer. The instrument uses an easy, quick dissociation curve and a dissociation protocol. To confirm the components of PCR, PCR samples were processed using the help of a positive control.
Chu et. al. were able to detect SARS-CoV-2 using the TaqMan QPCR probe. 2020. The qRT-PCR assay that used ORF1b nsp14 as the reference target had less specificity than the TaqMan-based reference protocol. The SYBR-based qPCR was found to be in line with the reference probe-based protocol.
The lncRNA GLIS2-AS1and the lncRNA P together with lncRNAs PVT1, are expressed in different ways in rat parathyroid carcinoma. The results of this study provide a co-expression network of lncRNAs and MRNAs. They also may help in future studies on parathyroid tumours.
In this study, we used ISH to identify the genes that express lncRNAs. We then verified these lncRNAs through comparing the expression patterns of benign parathyroid tumors and malignant tumors. The analysis integrated revealed a significant distinction between the expression patterns of LncRNAs from PAd and PCa. As possible markers for the differentiating of PAd and PCa, we propose lncRNAs GLIS2-AS1 (and lncRNA PVT1) The authors declare that they have no conflicts of interest.
The study also revealed a significant difference in BRC tumors with lncRNAST7 AS1 showing significant decreases in expression in breast cancer. The expression of lncRNAST7AS1 was also compared with normal breast tissue in the TCGA or GTEx databases.
The lncRNAs GLIS2-AS1, GLIS2-AS2 and lncRNA PVT1 were previously identified as differentially expressed lncRNAs in the glioblastoma. These results suggest that these genes exist in the brain and could be used to determine the condition in a patient.
The benefits of using the SIX3 Marker are many. These genes are beneficial for analyzing gene expression, and can also be used to track the effects of mutations on cell lines. The SIX3 gene is found in more than 150 human cell lines. It has been shown to affect the expression of HPE mRNA. The SIX3 marker can be found at GeneDx in Gaithersburg, MD, USA.
PMID: 9889003 by Granadino B., et al. Genomic cloning, structure, expression pattern, and chromosomal location of the human SIX3 gene.
PMID: 10415461 by Leppert G.S., et al. Sequence and location of SIX3, a homeobox gene expressed in the human eye.