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Product Info Summary
|Description:||Transient overexpression lysate of SIX homeobox 3 (SIX3)|
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SIX3 (NM_005413) Human Over-expression Lysate
Transient overexpression lysate of SIX homeobox 3 (SIX3)
Storage & Handling
Cite This Product
SIX3 (NM_005413) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006496)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SIX3 Human Over-expression Lysates NM_005413.
Western blot validation of overexpression lysate (LS006496) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SIX3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SIX3 (Source: Uniprot.org, NCBI)
Homeobox protein SIX3
SIX/Sine oculis homeobox family
holoprosencephaly 2, alobar or semilobar; homeobox protein SIX3; HPE2; sine oculis homeobox homolog 3 (Drosophila); Sine oculis homeobox homolog 3; SIX homeobox 3 SIX3 HPE2 SIX homeobox 3 homeobox protein SIX3|sine oculis homeobox homolog 3|sine oculis homeobox-like protein 3|truncated SIX3*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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