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- Table of Contents
SLC17A6 / Vesicular glutamate transporter 2 · Western blot design guide
Design a Western Blot for SLC17A6
Source-linked SLC17A6 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SLC17A6 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SLC17A6 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~64.4 kDa | |
| Observed band | ~64 kDa | |
| Gel | 5–20% (catalog A04376-1) | |
| Positive control | Cerebellum (IHC candidate; verify WB) +2 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked SLC17A6 Western Blot Protocol Options
The A04376-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human SH-SY5Y, human U-87MG, human U251, rat C6, mouse Neuro-2a (catalog A04376-1) |
| Gel % | 5–20% (catalog A04376-1) |
| Load | 30 ug; reducing conditions (catalog A04376-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A04376-1) |
| Membrane | nitrocellulose membrane (catalog A04376-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A04376-1) |
| Primary antibody | A04376-1 · 0.5 μg/mL (catalog A04376-1) |
| Primary incubation | overnight at 4°C (catalog A04376-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A04376-1) |
| Secondary incubation | 1.5 hour at RT (catalog A04376-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A04376-1) |
| Detection | ECL (catalog A04376-1) |
Section 2
What Is the Expected SLC17A6 Western Blot Band Size?
SLC17A6 is predicted at 64.4 kDa and observed near 64 kDa; the cause of their small difference is not established.
| Band near 64 kDa | Consistent with the approximately 64 kDa empirical SLC17A6 band; confirm identity with controls |
| Band above 64 kDa | Could reflect N-linked glycosylation at Asn100, Asn101, or Asn470; a visible shift is not established |
| Broad band near 64 kDa | Could reflect differing N-linked glycoforms; the listed sites do not establish a smear |
| Weak band in whole-cell lysate | SLC17A6 resides in synaptic vesicle and other membranes, so its signal may depend on membrane abundance |
💡Expected SLC17A6 appearanceSLC17A6 has a predicted mass of 64.4 kDa and an empirical band near 64 kDa; the listed glycosylation sites do not establish a migration shift, so confirm band identity with appropriate controls.
| Predicted SLC17A6 mass | 64.4 kDa predicted; the empirical band is approximately 64 kDa |
| N-linked glycosylation at Asn100 | May affect apparent mass if occupied; no shift is established |
| N-linked glycosylation at Asn101 | May affect apparent mass if occupied; no shift is established |
| N-linked glycosylation at Asn470 | May affect apparent mass if occupied; no shift is established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | SLC17A6 is a synaptic vesicle membrane protein and may be scarce in the sampled lysate | Check a positive sample and assess membrane or vesicle enrichment |
| Band higher than expected | N-linked glycosylation could affect migration, but the listed sites do not establish a visible shift | Compare with a positive control and assess band identity |
| Broad smear instead of sharp band | Differing N-linked glycoforms are possible, but a smear is not established | Check sample quality and compare with a positive control |
| Multiple bands | The listed glycosylation sites could contribute to differing forms, but distinct bands are not established | Compare with a positive control and verify antibody specificity |
| Weak or no signal | Low abundance of this vesicle membrane protein in the sample is possible | Use a positive control and assess membrane or vesicle enrichment |
Experiment evidence
Western blot images showing SLC17A6 band
A04376-1 · SLC17A6
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SLC17A6 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for SLC17A6 in Western blot, you can use cerebellum tissue.
Positive control: Cerebellum (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: As a synaptic vesicle membrane protein, SLC17A6 may be easier to detect in a synaptic vesicle enriched fraction than in whole-tissue lysate.
HPA tissue expression evidence for SLC17A6
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | processes in granular layer | High | Protein (IHC) | HPA → |
| Cerebral cortex | neuropil | High | Protein (IHC) | HPA → |
| Pancreas | pancreatic endocrine cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced SLC17A6 Western Blot Tips
Deeper troubleshooting and optimisation questions for SLC17A6, answered from its protein features.
How should SLC17A6 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Do annotated isoforms explain multiple SLC17A6 bands?
Isoforms · The supplied record reports one isoform and no alternative sequence. It therefore provides no isoform-based explanation for multiple bands; assess those bands experimentally before assigning them to SLC17A6.
Could glycosylation change the SLC17A6 band?
PTM · UniProt annotates N-linked glycosylation at Asn100, Asn101, and Asn470, using its sequence numbering. Compare untreated and deglycosylated samples if glycosylation is a concern. The annotations alone do not predict a visible shift.
Does this guide establish induction of SLC17A6?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for SLC17A6 Western blot?
Transfer · The features identify a 64.4 kDa multi-pass membrane protein but do not specify a transfer method. Check transfer efficiency and membrane retention around 64 kDa with your chosen conditions.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A04376-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should SLC17A6 bands be quantified?
Quantitation · Quantify a consistently defined band near the observed 64 kDa position across samples. Because SLC17A6 is membrane associated, keep fraction preparation consistent and verify that the signal remains within the assay’s linear range.
Why is the SLC17A6 band near 64 kDa?
Interpretation · The observed band near 64 kDa is close to the predicted 64.4 kDa mass. This agreement does not establish whether annotated glycosylation sites are occupied.
SLC17A6 is annotated as a multi-pass protein in synaptic vesicle membranes, with synaptosome and cell-membrane localization also listed. Include an appropriate membrane-containing fraction when checking whether the target is present.
The record lists three N-linked glycosylation sites but only one isoform. Neither feature alone identifies an unexpected band. Compare membrane-containing fractions and, where appropriate, deglycosylated samples before attributing another band to SLC17A6.
SLC17A6 is annotated as a multi-pass protein in synaptic vesicle membranes, with synaptosome and cell-membrane localization also listed. Include an appropriate membrane-containing fraction when checking whether the target is present.
The record lists three N-linked glycosylation sites but only one isoform. Neither feature alone identifies an unexpected band. Compare membrane-containing fractions and, where appropriate, deglycosylated samples before attributing another band to SLC17A6.
Boster reagents
SLC17A6 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-VGLUT2/SLC17A6 Antibody Picoband®
Cat # A04376-1
The catalog reports one anti-SLC17A6 Western blot antibody, A04376-1, with stated human, mouse, and rat reactivity. Its WB image reports an approximately 64 kDa band in human SH-SY5Y, U-87MG, and U251, rat C6, and mouse Neuro-2a lysates. Independent validation is not supplied.
Which to pick: A04376-1 is the only listed option. Its WB image uses the five named cell lysates at 30 µg per lane and 0.5 µg/mL primary antibody. Use those reported samples and conditions to judge its fit for your experiment.
Source: BosterBio SLC17A6 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9P2U8.
- Human Protein Atlas. SLC17A6 tissue expression.
- PMC11126684 — target-verified WB comparison
- PMC5634391 — target-verified WB comparison
- PMC11972371 — target-verified WB comparison