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- Table of Contents
SLC27A2 / Long-chain fatty acid transport protein 2 · Western blot design guide
Design a Western Blot for SLC27A2
Source-linked SLC27A2 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SLC27A2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SLC27A2 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~70.3 kDa | |
| Observed band | ~70 kDa | |
| Gel | 10% (catalog A07140-1) | |
| Positive control | Kidney (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked SLC27A2 Western Blot Protocol Options
The A07140-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Hacat, human HEL, human A549, human Hela, rat liver, rat kidney, mouse liver, mouse kidney (catalog A07140-1) |
| Gel % | 10% (catalog A07140-1) |
| Load | 30 ug; reducing conditions (catalog A07140-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A07140-1) |
| Membrane | nitrocellulose membrane (catalog A07140-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A07140-1) |
| Primary antibody | A07140-1 · 1: 1000 (catalog A07140-1) |
| Primary incubation | overnight at 4°C (catalog A07140-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A07140-1) |
| Secondary incubation | 1.5 hour at RT (catalog A07140-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A07140-1) |
| Detection | ECL (catalog A07140-1) |
Section 2
What Is the Expected SLC27A2 Western Blot Band Size?
SLC27A2 is predicted at 70.3 kDa and observed at ~70 kDa; the cause of the small difference is not established.
| Band at ~70 kDa | Matches the empirical SLC27A2 band near its predicted 70.3 kDa mass |
| Additional band near ~70 kDa | Could reflect isoform 1 or 2, but distinct migration is unestablished |
| Faint band in a soluble fraction | SLC27A2 is associated with endoplasmic reticulum and peroxisome membranes |
| Subtle doublet near ~70 kDa | Could reflect modification states, though a visible shift is unestablished |
💡Expected SLC27A2 appearanceSLC27A2 is predicted at 70.3 kDa and observed at ~70 kDa; confirm band identity with appropriate positive and negative controls.
| UniProt predicted mass | Places the reference size at 70.3 kDa, close to the observed ~70 kDa band |
| Isoforms 1 and 2 | May differ in size, but their masses and separation on a blot are unspecified |
| N6-acetyllysine at residue 291 | Has no established visible effect on band position |
| Phosphothreonine at residue 577 | Has no established visible effect on band position |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Membrane-associated SLC27A2 may be poorly recovered | Check a membrane-enriched fraction and a positive control |
| Band higher than expected | Incomplete solubilization of membrane-associated protein is possible | Review sample preparation and compare with the ~70 kDa positive-control band |
| Band lower than expected | Isoform identity or degradation is unresolved | Compare an independent antibody epitope and check sample integrity |
| Multiple bands | Isoforms 1 and 2 are annotated, but distinct bands are unproven | Compare both bands with a validated positive control and an independent antibody |
| Weak or no signal | Membrane-associated protein may be underrepresented in the prepared sample | Check membrane recovery and antibody performance with a positive control |
Experiment evidence
Western blot images showing SLC27A2 band
A07140-1 · SLC27A2
A07140-1 · SLC27A2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SLC27A2 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for SLC27A2 in Western blot, you can use kidney tissue, which shows high HPA expression.
Positive control: Kidney (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: As a membrane-associated protein, SLC27A2 may be easier to detect in a membrane-enriched lysate.
HPA tissue expression evidence for SLC27A2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Kidney | proximal tubules (cell body) | High | Protein (IHC) | HPA → |
| Bronchus | ciliated cells (cell body) | Medium | Protein (IHC) | HPA → |
| Epididymis | glandular cells | Medium | Protein (IHC) | HPA → |
| Fallopian tube | ciliated cells (ciliary rootlets) | Medium | Protein (IHC) | HPA → |
| Liver | hepatocytes | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced SLC27A2 Western Blot Tips
Deeper troubleshooting and optimisation questions for SLC27A2, answered from its protein features.
How should SLC27A2 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could SLC27A2 isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2. Isoform 2 lacks residues 230–282 of the canonical sequence, so a lower band is plausible. Confirm band identity with an antibody whose epitope is known for each isoform.
Which recorded modifications matter when interpreting SLC27A2 bands?
PTM · UniProt records N6-acetyllysine at position 291 and phosphothreonine at position 577, using canonical sequence coordinates. Neither feature alone demonstrates a visible band shift. Check the numbering convention before comparing these sites with antibody or paper annotations.
Does this guide establish induction of SLC27A2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for SLC27A2 Western blot?
Transfer · SLC27A2 is annotated as a membrane protein at the endoplasmic reticulum and peroxisome. Check transfer efficiency around 70 kDa with your membrane preparation and optimize the method empirically; the supplied features do not specify a transfer method.
How should blocking be optimized for SLC27A2?
Blocking · SLC27A2 is associated with the endoplasmic reticulum and peroxisome membranes. Compare blocking conditions using the same membrane preparation and assess background alongside the band near 70 kDa; the supplied features do not identify a preferred blocker.
How should SLC27A2 bands be quantified across samples?
Quantitation · Quantify the band near 70 kDa within the assay’s linear range and compare equivalent sample preparations. Because SLC27A2 localizes to endoplasmic reticulum and peroxisome membranes, differences in fraction preparation can affect the measured signal.
Does the observed band match SLC27A2’s predicted mass?
Interpretation · The reported band near 70 kDa agrees with the predicted 70.3 kDa mass. A small difference between apparent and calculated mass does not, by itself, identify a modification.
A lower band could be consistent with isoform 2, which lacks canonical residues 230–282, but its position alone cannot establish identity. The recorded acetylation at 291 and phosphorylation at 577 do not establish a visible shift; verify unexpected bands with epitope or isoform information.
A lower band could be consistent with isoform 2, which lacks canonical residues 230–282, but its position alone cannot establish identity. The recorded acetylation at 291 and phosphorylation at 577 do not establish a visible shift; verify unexpected bands with epitope or isoform information.
Boster reagents
SLC27A2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-FATP2/SLC27A2 Antibody Picoband®
Cat # A07140-1
The catalog reports one anti-SLC27A2 antibody for Western blot, A07140-1. Its product image shows a band near 70 kDa, versus an expected 65 kDa, in the human cell and rat and mouse tissue lysates named in the caption. No publication evidence was supplied.
Which to pick: A07140-1 is the only listed option. It reports human, mouse, and rat reactivity and has a Western blot image using human cell lysates and rat and mouse liver and kidney lysates. Match your sample and conditions to those shown.
Source: BosterBio SLC27A2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O14975.
- Human Protein Atlas. SLC27A2 tissue expression.
- PMC10091540 — target-verified WB comparison
- PMC11053221 — target-verified WB comparison
- PMC8761402 — target-verified WB comparison