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- Table of Contents
SLC7A1 / High affinity cationic amino acid transporter 1 · Western blot design guide
Design a Western Blot for SLC7A1
Real validated SLC7A1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SLC7A1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SLC7A1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~67.6 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Adrenal gland (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated | |
| Caveat | Glycosylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated SLC7A1 Western Blot Protocols
The PA2245 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Human Placenta, HELA, SKOV-3 (catalog PA2245) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | PA2245; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected SLC7A1 Western Blot Band Size?
SLC7A1 has a predicted mass of 67.6 kDa; two N-linked sites could affect migration, but no empirical band size or visible shift is established.
| Band near 67.6 kDa | Consistent with the predicted protein mass; identity requires confirmation. |
| Band above 67.6 kDa | N-linked glycans at Asn226 or Asn235 could increase apparent mass. |
| Several bands near the expected region | Glycosylation differences are possible, but distinct forms are unconfirmed. |
| Little or no band in a soluble fraction | SLC7A1 is a multi-pass cell membrane protein. |
💡Expected SLC7A1 appearanceThe predicted mass is 67.6 kDa; N-linked sites at Asn226 and Asn235 could affect migration, but no empirical band size is supplied, so confirm any detected band with identity controls.
| UniProt predicted mass | Sets a 67.6 kDa reference, not a measured migration position. |
| N-linked glycosylation at Asn226 | Could increase apparent mass if occupied; the shift is unknown. |
| N-linked glycosylation at Asn235 | Could increase apparent mass if occupied; the shift is unknown. |
| Two annotated N-linked glycosylation sites | Variable occupancy could alter migration, but a visible pattern is unproven. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Poor recovery of this multi-pass membrane protein | Check membrane extraction and a positive-control lysate. |
| Band higher than expected | Possible N-linked glycosylation at Asn226 or Asn235 | Compare untreated and deglycosylated samples with identity controls. |
| Broad smear instead of sharp band | Possible glycan heterogeneity; the annotated sites alone do not establish a smear | Compare deglycosylated and untreated samples. |
| Multiple bands | Possible glycosylation differences or unrelated antibody binding | Compare deglycosylated samples and use a SLC7A1 loss-of-signal control. |
| Weak or no signal | Incomplete extraction or transfer of the membrane protein | Check membrane enrichment, extraction, transfer, and a positive control. |
Experiment evidence
Western blot images showing SLC7A1 band
PA2245 · SLC7A1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SLC7A1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for SLC7A1 in Western blot, you can use adrenal gland tissue, which HPA scores as High.
Positive control: Adrenal gland (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: As a multi-pass membrane protein, SLC7A1 may be easier to detect in a membrane-enriched fraction.
HPA tissue expression evidence for SLC7A1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | glandular cells | High | Protein (IHC) | HPA → |
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Colon | endothelial cells | High | Protein (IHC) | HPA → |
| Duodenum | glandular cells | High | Protein (IHC) | HPA → |
| Endometrium | glandular cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
| Cerebellum | cells in granular layer | Not detected | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Hippocampus | glial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced SLC7A1 Western Blot Tips
Deeper troubleshooting and optimisation questions for SLC7A1, answered from its protein features.
How should SLC7A1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could annotated isoforms explain multiple SLC7A1 bands?
Isoforms · The supplied features list one isoform and no alternative sequence. They therefore provide no annotated isoform explanation for multiple bands.
Which glycosylation sites should guide band interpretation?
PTM · UniProt lists N-linked glycosylation at Asn226 and Asn235. These are UniProt sequence coordinates; antibody or paper numbering may differ. Compare bands across matched preparations before attributing a mobility difference to glycosylation.
Does this guide establish induction of SLC7A1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for SLC7A1 Western blot?
Transfer · SLC7A1 is a 629-residue multi-pass cell-membrane protein. Check transfer efficiency for the membrane-protein fraction and around the predicted 67.6 kDa region. The supplied features do not specify a preferred transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the PA2245 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should SLC7A1 bands be quantified?
Quantitation · Quantify the same consistently identified band across comparable samples and membrane preparations. Record which band was measured if several appear; the predicted 67.6 kDa mass and annotated glycosylation sites alone cannot identify every band.
Why might SLC7A1 migrate differently from its predicted 67.6 kDa mass?
Interpretation · SLC7A1 has two annotated N-linked glycosylation sites, Asn226 and Asn235, and is a multi-pass membrane protein. These features make the calculated mass an imperfect guide to migration, but they do not establish a visible shift or an expected apparent mass.
Assess them against the predicted 67.6 kDa mass, the N-linked sites at Asn226 and Asn235, and SLC7A1’s multi-pass membrane location. The supplied features list no alternative sequence or modified residues beyond glycosylation. They do not establish the identity of an unexpected band.
Assess them against the predicted 67.6 kDa mass, the N-linked sites at Asn226 and Asn235, and SLC7A1’s multi-pass membrane location. The supplied features list no alternative sequence or modified residues beyond glycosylation. They do not establish the identity of an unexpected band.
Boster reagents
SLC7A1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-CAT1/SLC7A1 Antibody Picoband®
Cat # PA2245
the supplier PA2245 is an anti-CAT1/SLC7A1 antibody listed as reactive with human samples. Its Western blot image shows human placenta tissue lysate and HeLa, SKOV-3, and HT1080 cell lysates; these examples do not establish performance in other samples.
Which to pick: PA2245 is the only listed option. It has a Western blot image for the named human tissue and cell lysates, making those the most directly documented contexts for choosing it.
Source: BosterBio SLC7A1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P30825.
- Human Protein Atlas. SLC7A1 tissue expression.
- PMC11752724 — target-verified WB comparison
- PMC12583633 — target-verified WB comparison
- PMC7644817 — target-verified WB comparison