This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
SNAPC5 / snRNA-activating protein complex subunit 5 · IHC design guide
Design Immunohistochemistry for SNAPC5
Plan chromogenic SNAPC5 IHC on paraffin sections using the catalog antibody at 1:100–1:300 (datasheet). Interpret the reported cytoplasmic tissue staining (HPA tissue IHC) alongside the nuclear molecular annotation (UniProt), with caution because tissue staining reliability is uncertain (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
SNAPC5 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic tissue stain (HPA tissue IHC); nuclear location (UniProt) | |
| Staining pattern | Cytoplasmic staining in most tissues (HPA tissue IHC) | |
| Antigen retrieval | Tris-EDTA pH 9.0 HIER, 95–98 °C, 20 min (rule: nuclear antigen) | |
| Positive control | Appendix+4 more · see all | |
| Negative control | Adipose tissue+3 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent across paraffin sections (standard IHC practice; not target-specific) | |
| Caveat | Tissue staining has low concordance with RNA expression (HPA tissue IHC) | |
| Regulation | Regulation unannotated (UniProt) | |
| Isoform / epitope | 2 isoforms; epitope differences unannotated (UniProt) |
Section 1
Recommended SNAPC5 IHC & IF Protocols
The catalog antibody’s IHC-P protocol is followed by one published SNAPC5 IHC example in human colon cancer tissue (PMC10373975).
| Sample | Paraffin-embedded human brain tissue; fixative not specified (datasheet A07260) |
| Fixation | Image fixative and duration unreported (datasheet A07260); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat-induced epitope retrieval in Tris-EDTA buffer, pH 9.0, 20 min at 95–98 °C (standard rule: nuclear antigen) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% normal serum of the secondary host, 30 min, room temperature (standard) |
| Primary antibody | Rabbit anti-SNAPC5, 1:100 - 1:300 (datasheet A07260) |
| Primary incubation | Overnight at 4 °C (standard) |
| Detection | HRP-polymer secondary, DAB chromogen 5–10 min (standard) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | SNAPC5-positive staining in glandular cells of appendix (HPA tissue IHC: Medium). HPA tissue profile: Cytoplasmic expression in most tissues. No signal in the no-primary control. |
💡Decision noteStart with Tris-EDTA pH 9.0 retrieval at 95–98 °C for 20 min (page retrieval rule; UniProt: nuclear localization).
Section 2
What Is the Expected SNAPC5 Staining Pattern?
SNAPC5 is a nuclear protein with no transmembrane segment (UniProt O75971: subcellular location and topology). Nucleoplasmic signal is supported by HPA ICC-IF (HPA: nucleoplasm, supported), while tissue IHC reports mainly cytoplasmic staining with uncertain reliability (HPA: tissue IHC, Uncertain). On paraffin sections, inspect the reported medium staining in appendix, breast, and colon glandular cells and in bronchial respiratory epithelial cells, but treat compartment assignment cautiously (HPA: tissue IHC).
| Distinct nuclear signal in glandular or respiratory epithelial cells, with a visible nuclear counterstain. | This fits the nuclear localization assigned by UniProt and supported by HPA ICC-IF (UniProt O75971: nucleus; HPA: nucleoplasm, supported). These cell types have medium tissue IHC staining, although that IHC profile describes the signal as cytoplasmic and rates its reliability Uncertain (HPA: tissue IHC). Record the compartment actually seen. |
| Cytoplasmic staining dominates, with little discernible nuclear signal. | This resembles HPA's tissue IHC profile, which reports cytoplasmic expression in most tissues (HPA: tissue IHC). It conflicts with the nuclear localization evidence (UniProt O75971: nucleus; HPA: nucleoplasm, supported). Because HPA rates the tissue IHC profile Uncertain, cytoplasmic signal alone does not establish SNAPC5 localization; assess background and antibody specificity. |
| Strong staining appears in adipocytes, smooth muscle cells, or soft-tissue fibroblasts. | These cell populations are listed as not detected in HPA tissue IHC (HPA: adipose tissue, smooth muscle, soft tissue). Unexpected staining raises possible cross-reactivity or endogenous detection activity, both general IHC concerns (standard IHC practice). Compare with an appropriate detection-only control before assigning the signal to SNAPC5. |
| Color covers nuclei, cytoplasm, and surrounding tissue without clear cell boundaries. | This is difficult to score as a cellular pattern and can reflect nonspecific staining or excess detection background (standard IHC practice). It cannot resolve the disagreement between nuclear localization and the uncertain cytoplasmic tissue profile (UniProt O75971: nucleus; HPA: tissue IHC, Uncertain). Interpret only compartment-resolved staining. |
| No signal is visible in appendix, breast, or colon glandular cells, or bronchial respiratory epithelial cells. | These are reported at medium intensity, making them useful comparison cells, although HPA rates the tissue IHC profile Uncertain (HPA: tissue IHC). Absence of signal can reflect assay performance or a difference between the specimen and HPA observations (standard IHC practice). Check the control and detection workflow before calling the specimen negative. |
💡Expected SNAPC5 appearanceA cautious positive call is discernible nuclear signal in a reported medium-staining epithelial cell population (UniProt O75971: nucleus; HPA: nucleoplasm, supported; HPA: appendix, breast, colon, or bronchus, Medium); cytoplasmic-only or widespread cell-free color is insufficiently specific (HPA: tissue IHC, Uncertain; standard IHC practice).
| Localization evidence | UniProt places SNAPC5 in the nucleus, and HPA ICC-IF supports nucleoplasmic localization (UniProt O75971; HPA: subcellular). HPA tissue IHC instead describes cytoplasmic expression and rates its profile Uncertain (HPA: tissue IHC). This disagreement limits how confidently a chromogenic compartment can be assigned. |
| Tissue and cell context | HPA reports medium staining in glandular, respiratory epithelial, and selected neuronal populations; adipocytes, smooth muscle cells, and soft-tissue fibroblasts are not detected (HPA: tissue IHC). These are observed levels, not a universal positive or negative guarantee: HPA reports low tissue RNA specificity and uncertain IHC reliability (HPA: tissue IHC). |
| Antibody validation | The listed antibody, HPA024379, is rated Uncertain for IHC and Supported for ICC (HPA: antibodies). The tissue profile also has low consistency between antibody staining and RNA expression data and awaits external verification (HPA: tissue IHC). Treat an unexpected IHC compartment or cell population as provisional. |
| Isoforms and epitope scope | UniProt lists two SNAPC5 isoforms but supplies no epitope position for the antibody here (UniProt O75971: isoforms; HPA: antibodies). The record therefore cannot show whether both isoforms are recognized or whether an isoform difference explains a tissue result. Avoid assigning an isoform from stain intensity alone. |
| Topology, processing, and modification | SNAPC5 has no transmembrane segment or signal peptide; its annotated chain spans residues 1–98, with phosphothreonine at residue 85 (UniProt O75971: topology, processing, modified residues). The supplied evidence gives no antibody epitope or target-specific fixation effect, so these features do not predict retrieval needs, staining intensity, or antigen loss. |
| Situation | Likely cause | Next action |
|---|---|---|
| A reported medium-staining tissue has no visible signal. | The assay may have failed, or the specimen may differ from HPA's observation (standard IHC practice; HPA: tissue IHC, Uncertain). | Confirm that the positive control and detection reagents worked, then review antibody dilution and the chosen antigen-retrieval procedure as general IHC checks (standard IHC practice). Do not infer SNAPC5-specific retrieval or fixation sensitivity from this record. |
| Only cytoplasmic staining appears. | HPA reports cytoplasmic tissue staining, but its IHC reliability is Uncertain and nuclear localization has separate support (HPA: tissue IHC; HPA: nucleoplasm, supported; UniProt O75971: nucleus). | Score nuclear and cytoplasmic signal separately. Compare with a detection-only control and, where available, independent specificity evidence (standard IHC practice); describe cytoplasmic-only staining as unresolved rather than confirmed SNAPC5. |
| Staining appears in a population listed as not detected. | Cross-reactivity or endogenous detection activity is possible (standard IHC practice); HPA lists adipocytes, smooth muscle cells, and soft-tissue fibroblasts as not detected (HPA: tissue IHC). | Run a suitable control without the primary antibody and inspect whether the color follows the detection system (standard IHC practice). Reassess antibody-specific signal before changing the expected tissue pattern. |
| Diffuse color obscures cells or compartments. | Nonspecific binding or detection background can obscure localization (standard IHC practice). The uncertain HPA tissue IHC profile cannot validate an indistinct signal (HPA: tissue IHC, Uncertain). | Inspect controls, blocking, washing, antibody dilution, and detection exposure; adjust only the implicated workflow step (standard IHC practice). Score the slide once cell boundaries and the nuclear counterstain can be distinguished. |
| A negative comparator stains as strongly as the test population. | The result conflicts with HPA's not-detected calls for the specified comparator cells, though the IHC profile itself is Uncertain (HPA: tissue IHC). | Verify the cell identity and compare staining with the detection-only control (standard IHC practice). Report the discordance explicitly; HPA's uncertain profile does not justify calling either observation definitive. |
| Does IF/ICC clarify the expected compartment? | HPA ICC-IF supports a mainly nucleoplasmic location, whereas HPA tissue IHC reports cytoplasmic expression with Uncertain reliability (HPA: subcellular; HPA: tissue IHC). | Use the ICC-IF result as localization context when interpreting the IHC slide, while keeping the assays' evidence separate (standard IHC practice). The supplied ICC-IF evidence provides no IF/ICC protocol or basis for an IF reagent setting. |
Experiment evidence
IHC & IF images showing SNAPC5 staining
A07260 · IHCImmunohistochemistry analysis of paraffin-embedded human brain tissue, using SNAPC5 Antibody. The picture on the right is blocked with the s
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000174446-SNAPC5/tissue/cerebral+cortex#imid_15294226
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000174446-SNAPC5/tissue/colon#imid_15294152
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000174446-SNAPC5/tissue/liver#imid_15294153
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000174446-SNAPC5/tissue/kidney#imid_15294146
HPA · ICC-IF A-549Human Protein Atlas immunofluorescence, A-549, antibody HPA024379; green = SNAPC5, blue = nucleus, red = microtubules.
HPA · ICC-IF A-549Human Protein Atlas immunofluorescence, A-549, antibody HPA024379; green = SNAPC5, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for SNAPC5 IHC & IF
🧪Run breast first: glandular cells should stain at a medium level (HPA: Breast glandular cells, Medium). Use adipose tissue as the negative tissue (HPA: Adipocytes, Not detected); adipocytes within the breast section, if present, should lack specific nuclear staining and can serve as internal negative cells (HPA: Adipocytes, Not detected; UniProt O75971: Nucleus).
Positive control tissue: Appendix (Glandular cells, HPA Medium)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show SNAPC5 in A-549, SK-MEL-30, U2OS, with annotated localisation: Nucleoplasm (supported) (HPA subcellular).
Technical controls: Include no-primary (secondary-only) and host-species-matched isotype controls for a monoclonal antibody, or matched nonimmune IgG for a polyclonal antibody (standard IHC practice). Use SNAPC5 knockout tissue if available or immunizing-peptide competition as a biological specificity control (selected SKU A07260 tissue-IHC caption: peptide block); quench endogenous peroxidase for chromogenic detection and block endogenous biotin if using avidin–biotin detection (standard IHC practice).
⚠️Feasibility: A target-specific fixation window, fixation effect, and antigen-retrieval dependency are unreported in the supplied evidence; the selected SKU A07260 paraffin-section tissue-IHC caption does not state a fixative. Paraffin-section IHC is documented in that caption, while HPA documents nucleoplasmic ICC-IF staining; the evidence does not establish whether frozen-section staining or IF is easier (selected SKU A07260 tissue-IHC caption; HPA subcellular: Nucleoplasm, supported). In breast sections, adipocytes may provide an internal negative comparison if present, but assess background in the glandular compartment separately (HPA: Breast glandular cells, Medium; HPA: Adipocytes, Not detected).
HPA tissue IHC evidence for SNAPC5
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Uncertain — Low consistency between antibody staining and RNA expression data. Pending external verification.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | Glandular cells | Medium | Protein (IHC) | HPA → |
| Breast | Glandular cells | Medium | Protein (IHC) | HPA → |
| Bronchus | Respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
| Caudate | Neuronal cells | Medium | Protein (IHC) | HPA → |
| Cerebellum | Cells in granular layer | Medium | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | Smooth muscle cells | Not detected | Protein (IHC) | HPA → |
| Soft tissue | Fibroblasts | Not detected | Protein (IHC) | HPA → |
| Vagina | Squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced SNAPC5 IHC Tips
These questions address chromogenic IHC of SNAPC5 in paraffin sections, with one entry for interpreting related IF/ICC evidence.
How should I retrieve SNAPC5 in paraffin sections when nuclear staining is weak?
Use Tris-EDTA pH 9.0 heat-induced epitope retrieval at 95–98 °C for 20 min (page retrieval setting: nuclear-antigen rule). Allow sections to cool in buffer before washing, and hold section thickness, detection chemistry, and antibody incubation constant while comparing retrieval conditions (standard IHC practice). Evaluate signal in nuclei because SNAPC5 is annotated as nuclear and supported in the nucleoplasm (UniProt O75971; HPA subcellular). If staining remains weak, test a shorter or longer heating interval on adjacent sections while checking tissue morphology and background (standard IHC practice). Keep a no-primary control alongside each condition so increased chromogen deposition is not mistaken for improved antigen exposure (standard IHC practice).
Can fixation explain weak SNAPC5 staining in paraffin tissue?
Target-specific fixation sensitivity for SNAPC5 is unknown from the supplied evidence; the selected tissue image states paraffin embedding but does not report its fixative (A07260 caption). Record fixative, fixation duration, processing history, section age, and retrieval conditions for each specimen before comparing staining intensity (standard IHC practice). When archival and freshly prepared sections differ, stain them in the same run with the same antibody and detection conditions to limit procedural variation (standard IHC practice). Compare nuclear signal and tissue preservation together, because damaged morphology can make compartment assignment unreliable (standard IHC practice). Do not use the HPA tissue pattern or SNAPC5 topology and phosphorylation annotation to assign a target-specific fixation effect (HPA tissue IHC; UniProt O75971).
What compartment should count as convincing SNAPC5 staining?
Prioritise nuclear staining when scoring SNAPC5: UniProt lists a nuclear location, and HPA supports localisation mainly in the nucleoplasm (UniProt O75971; HPA subcellular). The complex participates in small nuclear RNA gene transcription, so a nuclear pattern is biologically coherent, although function alone cannot validate an IHC stain (UniProt O75971). HPA also reports cytoplasmic expression in most tissues, but labels its tissue IHC reliability uncertain because staining and RNA expression have low consistency (HPA tissue IHC). Score nuclear and cytoplasmic chromogen separately, with a counterstain that preserves nuclear boundaries (standard IHC practice). Confirm that apparent cytoplasmic signal persists away from folds, edges, and damaged regions before interpreting it (standard IHC practice).
Could isoforms or epitope masking change the SNAPC5 IHC pattern?
SNAPC5 has 2 annotated isoforms, but the supplied evidence does not map the catalog antibody epitope to either one (UniProt O75971; A07260 caption). Its annotated chain spans residues 1–98, and phosphorylation at threonine 85 is listed; neither fact establishes antibody recognition or retrieval sensitivity (UniProt O75971). Check the antibody's documented immunogen or epitope, if available, before interpreting differences between specimens as isoform-specific expression (standard IHC practice). Compare adjacent sections using the same retrieval and detection conditions, then assess nuclear staining and morphology together (standard IHC practice). Peptide blocking reduced the signal in the selected brain image, but that result does not identify an isoform or prove specificity in every tissue (A07260 caption).
How can IF help investigate an ambiguous SNAPC5 IHC pattern?
Use IF/ICC as a complementary localisation check: HPA supports SNAPC5 in the nucleoplasm and lists images from A-549, SK-MEL-30, and U2OS cells (HPA subcellular). For brain comparisons, multiplex with a neuronal cell marker where the IHC question concerns neurons, since HPA reports medium staining in neuronal cells of the caudate and cerebral cortex (HPA tissue IHC). Choose a far-red fluorophore when tissue autofluorescence compromises shorter-wavelength channels, and include single-colour controls (standard IF practice). Permeabilise sufficiently to expose an intracellular nuclear epitope, then check that nuclear boundaries remain intact; SNAPC5 has no annotated transmembrane segment (standard IF practice; UniProt O75971). Optimise IF fixation separately because no directly relevant SNAPC5 fixation condition is supplied (HPA subcellular).
How do I distinguish SNAPC5 signal from chromogenic background?
Run no-primary and detection-only controls alongside the catalog antibody to reveal nonspecific reagent signal (standard IHC practice). For peroxidase-based chromogenic detection, include a peroxidase block before DAB development and compare sections processed with identical development times (standard IHC practice). Assess precipitate at section edges, folds, vessels, and damaged areas before calling cells positive (standard IHC practice). The selected paraffin brain image shows peptide blocking, which supports a peptide-sensitive signal in that image but does not exclude every source of tissue background (A07260 caption). Treat widespread cytoplasmic staining cautiously because HPA reports that pattern while rating tissue IHC reliability uncertain (HPA tissue IHC).
How should I quantify SNAPC5 staining across tissue sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the anatomical region and cell population before scoring, and analyse equivalent regions across specimens (standard IHC practice). Report the percentage of nuclei in each intensity category and calculate an H-score as the sum of each percentage multiplied by its 0–3 intensity grade (standard IHC practice). Alternatively, report SNAPC5-positive cells per mm², normalised to analysable tissue area, when cell density is the principal outcome (standard IHC practice). Keep nuclear and cytoplasmic scores separate because supported nucleoplasmic localisation and uncertain cytoplasmic tissue staining lead to different interpretations (HPA subcellular; HPA tissue IHC). Exclude folds, necrosis, and poorly counterstained regions by a prespecified rule, and use identical imaging and threshold settings across slides (standard IHC practice).
When is a positive SNAPC5 IHC result credible?
A credible result has reproducible staining in intact nuclei, consistent with the supported nucleoplasmic location and SNAPC5's role in small nuclear RNA gene transcription (HPA subcellular; UniProt O75971). Check cell identity: HPA reports medium staining in cerebral cortex neurons and cerebellar granular-layer cells, while its overall tissue IHC reliability remains uncertain (HPA tissue IHC). Reject isolated signal at section edges, folds, or necrotic areas, and investigate deposits reproduced in no-primary controls or attributable to endogenous peroxidase (standard IHC practice). Peptide blocking in the selected paraffin brain image is useful supporting evidence, but its caption leaves fixation unreported and does not validate every specimen (A07260 caption). Resolve a predominantly cytoplasmic result with compartment-specific scoring and an independent validation approach before assigning it to SNAPC5 (HPA tissue IHC; HPA subcellular).
Boster reagents
Best SNAPC5 / snRNA-activating protein complex subunit 5 IHC Antibodies
A07260 has a human brain paraffin-section IHC image (catalog IHC caption); IF/ICC are listed applications without a supplied IF image (catalog applications; IF image alts).
Real IHC data
Anti-SNAPC5 Antibody
Cat # A07260
A07260 will render with an IHC image of paraffin-embedded human brain tissue and a peptide-blocked comparison (catalog IHC caption). Human and mouse reactivity and IF/ICC applications are listed, but no IF image is supplied (catalog reactivity; catalog applications; IF image alts).
Which to pick: Choose A07260 for tissue IHC because its own image shows paraffin-embedded human brain tissue; the fixative is unreported (catalog IHC caption). For IF/ICC, A07260 is the listed option, with a catalog IF dilution of 1:50, but no IF image is supplied (catalog applications; catalog IF dilution; IF image alts). For human or mouse samples, A07260 is the listed rabbit polyclonal option; its IHC image documents human tissue only (catalog reactivity; catalog host; catalog dilution_raw; catalog IHC caption).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry O75971 (SNPC5_HUMAN, snRNA-activating protein complex subunit 5).
- Human Protein Atlas. SNAPC5 tissue IHC expression (reliability: Uncertain).
- Human Protein Atlas. SNAPC5 subcellular location (ICC-IF): Mainly localized to the nucleoplasm..
- Human Protein Atlas. SNAPC5 antibody validation summary (1 antibodies).
- Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression. Aging 2023 — PMC10373975.
- PubMed PMID:9732265 — UniProt-cited evidence.
- PubMed PMID:14702039 — UniProt-cited evidence.
- PubMed PMID:15489334 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for SNAPC5 (snRNA-activating protein complex subunit 5). Expected localisation Cytoplasmic tissue stain (HPA tissue IHC); nuclear location (UniProt). Antigen retrieval: Tris-EDTA pH 9.0 HIER, 95–98 °C, 20 min (rule: nuclear antigen). Validated IHC antibody: A07260 at 1:100 - 1:300 (the product's own IHC validation image is shown). Controls (HPA): positive Appendix (Glandular cells); negative Adipose tissue. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human brain tissue; fixative not specified (datasheet A07260); 2. Fixation: Image fixative and duration unreported (datasheet A07260); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat-induced epitope retrieval in Tris-EDTA buffer, pH 9.0 , 20 min at 95–98 °C…; 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% normal serum of the secondary host, 30 min , room temperature (standard); 8. Primary antibody: Rabbit anti-SNAPC5, 1:100 - 1:300 (datasheet A07260); 9. Primary incubation: Overnight at 4 °C (standard). Watch for: Fixation: Keep fixation consistent across paraffin sections (standard IHC practice; not target-specific); Caveat: Tissue staining has low concordance with RNA expression (HPA tissue IHC); Isoform / epitope: 2 isoforms; epitope differences unannotated (UniProt); Regulation: Regulation unannotated (UniProt). Evidence: UniProt O75971, HPA tissue IHC · Uncertain, HPA ICC-IF · Mainly localized to the nucleoplasm., Datasheet A07260, PMC10373975. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/SNAPC5/immunohistochemistry-ihc-guide.