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- Table of Contents
SOS2 / Son of sevenless homolog 2 · Western blot design guide
Design a Western Blot for SOS2
Source-linked SOS2 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SOS2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SOS2 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~153 kDa | |
| Gel | 8–10% (standard starting point) | |
| Positive control | Bone marrow (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Methylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked SOS2 Western Blot Protocol Options
The A05957-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Hela, sp2/0, H9C2 (catalog A05957-1) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Wet/tank transfer; optimize duration (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A05957-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected SOS2 Western Blot Band Size?
SOS2 is predicted at 153 kDa; isoforms 1 and 2 could affect migration, but no empirical band or distinct isoform sizes are established.
| Band near 153 kDa | consistent with the predicted SOS2 size; confirm identity with controls |
| Single dominant band near 153 kDa | isoforms 1 and 2 may not resolve separately |
| Two bands near the expected region | could reflect isoforms 1 and 2 if their migration differs; confirm identity |
| Band at an unexpected position | size alone cannot establish which SOS2 isoform it represents |
💡Expected SOS2 appearanceSOS2 has a predicted mass of 153 kDa, but no empirical band size or isoform migration is supplied; confirm any candidate band with ordinary identity controls.
| UniProt predicted mass | places the reference size near 153 kDa without establishing observed migration |
| Alternative splicing | could change apparent size, but no isoform masses or migration differences are supplied |
| Isoform 1 | its individual apparent size is unknown |
| Isoform 2 | its size relative to isoform 1 is unknown |
| Situation | Likely cause | Next action |
|---|---|---|
| Multiple bands | isoforms 1 and 2 are annotated, but their migration is unknown | compare isoform-specific expression or depletion controls |
| Band higher than expected | the 153 kDa prediction does not establish migration or band identity | verify specificity with SOS2 depletion and a molecular weight marker |
| Band lower than expected | an isoform or unrelated signal is possible; no isoform mass is supplied | check epitope coverage and compare SOS2 depletion controls |
| Weak or no signal | SOS2 abundance or antibody recognition may be insufficient | use a positive lysate control and check antibody dilution and exposure |
| Fragments below expected size | sample degradation is possible; no SOS2 cleavage feature is listed | compare freshly prepared lysate with protease inhibition and verify SOS2 specificity |
Experiment evidence
Western blot images showing SOS2 band
A05957-1 · SOS2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SOS2 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for SOS2 in Western blot, you can use bone marrow lysate.
Positive control: Bone marrow (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA reports a not-detected tissue for a negative control, though SOS2 is high in several other tissues.
HPA tissue expression evidence for SOS2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bone marrow | hematopoietic cells | High | Protein (IHC) | HPA → |
| Cerebral cortex | neuronal cells | High | Protein (IHC) | HPA → |
| Duodenum | glandular cells | High | Protein (IHC) | HPA → |
| Hippocampus | neuronal cells | High | Protein (IHC) | HPA → |
| Lung | macrophages | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Liver | cholangiocytes | Not detected | Protein (IHC) | HPA → |
| Esophagus | squamous epithelial cells | Low | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | Low | Protein (IHC) | HPA → |
| Spleen | cells in white pulp | Low | Protein (IHC) | HPA → |
Section 3
Advanced SOS2 Western Blot Tips
Deeper troubleshooting and optimisation questions for SOS2, answered from its protein features.
How should SOS2 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could SOS2 isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2. Isoform 2 lacks residues 324–356 of the canonical sequence, so the isoforms may differ in size. The supplied features do not establish whether they resolve as separate bands.
Determine whether the antibody detects both isoforms: isoform 2 lacks canonical residues 324–356. If bands resolve, report them separately unless the measurement is intended to combine isoforms. The supplied features do not identify an antibody epitope.
Determine whether the antibody detects both isoforms: isoform 2 lacks canonical residues 324–356. If bands resolve, report them separately unless the measurement is intended to combine isoforms. The supplied features do not identify an antibody epitope.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of SOS2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for SOS2 Western blot?
Transfer · SOS2 has a predicted mass of 153 kDa. Check transfer efficiency in that size range using a stained membrane or the gel after transfer. The supplied features do not specify a transfer method or settings.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A05957-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should SOS2 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Should SOS2 migrate at exactly 153 kDa?
Interpretation · 153 kDa is the predicted mass. No observed band size is supplied, so use it as a reference, not an exact migration requirement. The listed features alone do not explain a difference between apparent and predicted mass.
UniProt lists asymmetric dimethylarginine at position 1321. This modification alone does not establish a visible shift or explain an unexpected band. Keep UniProt numbering explicit when comparing site descriptions.
Compare them with the 153 kDa prediction and the 33-residue deletion in isoform 2. Neither feature confirms a band's identity. The listed methylation at UniProt position 1321 does not, by itself, establish a visible band shift.
UniProt lists asymmetric dimethylarginine at position 1321. This modification alone does not establish a visible shift or explain an unexpected band. Keep UniProt numbering explicit when comparing site descriptions.
Compare them with the 153 kDa prediction and the 33-residue deletion in isoform 2. Neither feature confirms a band's identity. The listed methylation at UniProt position 1321 does not, by itself, establish a visible band shift.
Boster reagents
SOS2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Son of sevenless homolog 2 SOS2 Antibody
Cat # A05957-1
The catalog reports A05957-1, a polyclonal anti-SOS2 antibody with reported human, mouse, and rat reactivity. Its WB image shows HeLa, sp2/0, and H9C2 lysates at 1:500 dilution. No publication evidence or additional validation is supplied.
Which to pick: A05957-1 is the only listed option. Its WB image uses HeLa, sp2/0, and H9C2 lysates at 1:500; check whether those tested contexts and the reported reactivity fit your experiment.
Source: BosterBio SOS2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q07890.
- Human Protein Atlas. SOS2 tissue expression.
- PMC10327037 — target-verified WB comparison
- PMC7396125 — target-verified WB comparison
- PMC10920089 — target-verified WB comparison