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- Table of Contents
SOX8 / Transcription factor SOX-8 · Western blot design guide
Design a Western Blot for SOX8
Real validated SOX8 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SOX8 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SOX8 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~47.3 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | SOX family cross-reactivity | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated SOX8 Western Blot Protocols
The A06098 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | lysates from COS7 cells, treated with serum 10% 30', (catalog A06098) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | the synthesized peptide (catalog A06098) |
| Primary antibody | A06098; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected SOX8 Western Blot Band Size?
SOX8 is predicted at 47.3 kDa; nuclear localization may affect recovery, but no supplied feature demonstrates altered migration or an empirical band size.
| Single band near 47.3 kDa | Consistent with the predicted full-length SOX8 size; confirm identity with appropriate controls |
| Band enriched in the nuclear fraction | Consistent with SOX8 nuclear localization |
| Little or no band in the cytoplasmic fraction | Consistent with SOX8 nuclear localization |
| Several bands at different sizes | Cannot be assigned to SOX8 isoforms from the single annotated isoform |
💡Expected SOX8 appearanceSOX8 has a predicted full-length mass of 47.3 kDa, but no empirical band size is supplied; confirm a candidate band with nuclear enrichment and antibody specificity controls.
| UniProt predicted molecular weight | Sets a full-length reference of 47.3 kDa |
| UniProt mass of 47,314 Da | Expresses the same full-length reference in daltons |
| 446-amino-acid sequence | Defines the full-length polypeptide underlying the predicted mass |
| Single annotated isoform | Provides no supported alternative isoform size |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Nuclear SOX8 may be poorly recovered | Check nuclear recovery and probe a nuclear fraction |
| Band higher than expected | No supplied SOX8 feature establishes the shift | Check band identity with a SOX8-specific antibody or SOX8 depletion |
| Band lower than expected | No supplied processing feature explains the lower band | Check band identity and sample integrity |
| Multiple bands | One annotated isoform does not explain several sizes; the listed antibody also recognizes other SOX proteins | Use a SOX8-specific antibody or SOX8 depletion to identify the band |
| Weak or no signal | Nuclear SOX8 may be underrepresented in the sample | Check nuclear recovery and antibody performance |
| Fragments below expected size | Fragment identity is not established by the supplied features | Check sample integrity and validate the bands with SOX8 depletion |
Experiment evidence
Western blot images showing SOX8 band
A06098 · SOX8
A06098 · SOX8
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SOX8 Western blot
🧪For positive controls for SOX8 in Western blot, you can use a validated SOX8-positive sample once identified; the supplied HPA record names none.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: The supplied HPA record has no tissue data, so tissue controls cannot be selected from it.
HPA tissue expression evidence for SOX8
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced SOX8 Western Blot Tips
Deeper troubleshooting and optimisation questions for SOX8, answered from its protein features.
Where should the SOX8 band appear relative to its predicted mass?
Band shift · SOX8 has a predicted mass of 47.3 kDa. Use that as a reference when inspecting the blot; no empirical band position is supplied, so an apparent shift cannot be predicted from these features.
Could SOX8 isoforms account for multiple bands?
Isoforms · The supplied record lists one isoform and no alternative sequence. Do not assign additional bands to annotated SOX8 isoforms on this evidence alone.
Do annotated modifications explain a shifted SOX8 band?
PTM · No modified residues or glycosylation sites are listed. These features provide no basis for assigning a band shift to a specific modification; they also do not establish why an observed band might differ from 47.3 kDa.
Does this guide establish induction of SOX8?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for SOX8?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A06098 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should SOX8 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Which sample fraction is most relevant for detecting SOX8?
Interpretation · SOX8 is annotated as nuclear. Check a nuclear-containing sample when evaluating its signal, and account for the fraction used when comparing band intensity across samples.
Compare equivalent sample fractions because SOX8 is annotated as nuclear. Use the same band-identification criteria across samples; the supplied features do not establish an expected abundance or response to treatment.
No signal peptide or propeptide is listed. The supplied features therefore do not identify a processed SOX8 product or support assigning a smaller band to one.
Compare equivalent sample fractions because SOX8 is annotated as nuclear. Use the same band-identification criteria across samples; the supplied features do not establish an expected abundance or response to treatment.
No signal peptide or propeptide is listed. The supplied features therefore do not identify a processed SOX8 product or support assigning a smaller band to one.
Boster reagents
SOX8 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Sox-8/9/17/18 Antibody
Cat # A06098
A06098 is an anti-Sox-8/9/17/18 antibody with listed Human, Monkey, and Mouse reactivity. Its Western blot image shows serum-treated COS7 lysates and a peptide-blocked lane. The supplied evidence does not establish SOX8-specific detection.
Which to pick: A06098 is the only listed option and has a Western blot image from COS7 lysates treated with 10% serum for 30 minutes. Choose it when that evidence fits your experiment; confirm SOX8 specificity for your sample.
Source: BosterBio SOX8 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P57073.
- Human Protein Atlas. SOX8 tissue expression.
- PMC5589225 — target-verified WB comparison
- PMC12997667 — target-verified WB comparison
- PMC12412890 — target-verified WB comparison