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- Table of Contents
SUV39H1 / Histone-lysine N-methyltransferase SUV39H1 · Western blot design guide
Design a Western Blot for SUV39H1
Real validated SUV39H1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SUV39H1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SUV39H1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~47.9 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated SUV39H1 Western Blot Protocols
The A00954 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | extracts of various cell lines, (catalog A00954) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | Blocking buffer: 3% nonfat dry milk in TBST (catalog A00954) |
| Primary antibody | A00954 · 1:1000 (catalog A00954) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Goat Anti-Rabbit IgG, 1:10000 (catalog A00954) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL (catalog A00954) |
Section 2
What Is the Expected SUV39H1 Western Blot Band Size?
SUV39H1 is predicted at 47.9 kDa; annotated isoforms and modifications may affect migration, but no distinct band sizes or shifts are demonstrated.
| Band near 47.9 kDa | consistent with the predicted SUV39H1 mass; confirm its identity |
| Additional bands at different positions | isoforms 1 and 2 are annotated, but distinct migration is unverified |
| Weak band in whole-cell lysate | nuclear and chromosomal localization may affect recovery |
| Band with altered mobility | Lys266 acetylation or Ser391 phosphorylation is possible, but a visible effect is unproven |
💡Expected SUV39H1 appearanceSUV39H1 has a predicted mass of 47.9 kDa; no empirical band size is supplied, so confirm any candidate band with ordinary identity controls.
| Predicted SUV39H1 mass | 47.9 kDa provides the reference size, not a measured band |
| Isoforms 1 and 2 | may differ in size, but their masses and migration are not supplied |
| Lys266 acetylation | a measurable mobility effect is not established |
| Ser391 phosphorylation by CDK2 | a measurable mobility effect is not established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | nuclear or chromosomal SUV39H1 may be poorly recovered | check nuclear or chromatin-enriched extracts and use a positive control |
| Band higher than expected | band identity or modification-dependent migration is unresolved | verify with SUV39H1 knockdown and a size marker |
| Band lower than expected | an isoform or protein degradation is possible | compare with knockdown and prepare lysate with protease inhibitors |
| Multiple bands | isoforms 1 and 2 are annotated, but distinct bands are unverified | test band specificity by SUV39H1 knockdown |
| Weak or no signal | limited recovery of nuclear or chromosome-associated protein is possible | check extraction and loading with a nuclear control |
Experiment evidence
Western blot images showing SUV39H1 band
A00954 · SUV39H1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SUV39H1 Western blot
🧪For positive controls for SUV39H1 in Western blot, you can use a validated SUV39H1-positive sample, since no HPA positive tissue or cell is supplied.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA supplies no tissue controls, so validate a positive sample and use knockdown or KO for a negative control.
HPA tissue expression evidence for SUV39H1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced SUV39H1 Western Blot Tips
Deeper troubleshooting and optimisation questions for SUV39H1, answered from its protein features.
How should SUV39H1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could SUV39H1 isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2. In isoform 2, residues 1–6 (MAENLK) are replaced by MVGMSRLRNDRLADPLT, giving it a longer N-terminus. This sequence difference may affect migration, but the features do not establish whether two bands will resolve.
Check the antibody epitope against both sequences. Isoform 2 replaces isoform 1 residues 1–6, so an antibody recognizing that segment may detect the isoforms differently.
Check the antibody epitope against both sequences. Isoform 2 replaces isoform 1 residues 1–6, so an antibody recognizing that segment may detect the isoforms differently.
Which SUV39H1 modifications matter when interpreting bands?
PTM · UniProt lists N6-acetyllysine at Lys266 and CDK2-mediated phosphorylation at Ser391, using UniProt residue numbering. These features establish modification sites, not a visible band shift or an explanation for any mass difference. Check the numbering convention used for an antibody or paper before comparing sites.
UniProt identifies CDK2 as the kinase for Ser391 and lists cell cycle among SUV39H1's keywords. If comparing cell-cycle conditions, record them and assess Ser391 phosphorylation directly; the supplied features do not establish how any condition changes its level.
UniProt identifies CDK2 as the kinase for Ser391 and lists cell cycle among SUV39H1's keywords. If comparing cell-cycle conditions, record them and assess Ser391 phosphorylation directly; the supplied features do not establish how any condition changes its level.
Does this guide establish induction of SUV39H1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for SUV39H1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A00954 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should SUV39H1 be quantified across samples?
Quantitation · SUV39H1 is reported in the nucleus, on chromosomes, and at the nuclear lamina. Use comparable sample fractions and normalize measurements within the chosen fraction so differences in fraction recovery are not mistaken for differences in SUV39H1 abundance.
Should SUV39H1 migrate at exactly 47.9 kDa?
Interpretation · 47.9 kDa is the predicted mass; no observed band position is supplied. Compare the detected band with this estimate, but do not assign any difference to a modification without further evidence.
First check antibody recognition of both listed isoforms and compare bands with the 47.9 kDa predicted mass. Lys266 acetylation and Ser391 phosphorylation are documented sites, but neither establishes a visible shift. The supplied features and absent observed-band data cannot identify an unexpected band on their own.
First check antibody recognition of both listed isoforms and compare bands with the 47.9 kDa predicted mass. Lys266 acetylation and Ser391 phosphorylation are documented sites, but neither establishes a visible shift. The supplied features and absent observed-band data cannot identify an unexpected band on their own.
Boster reagents
SUV39H1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-SUV39H1 Antibody
Cat # A00954
A00954 is an anti-SUV39H1 antibody listed for human, mouse, and rat. Its Western blot image uses cell-line extracts at 1:1000 dilution with 25 µg protein per lane; the caption does not identify the cell lines or establish testing in each listed species.
Which to pick: A00954 is the only listed option. It has a Western blot image, so use its reported 1:1000 dilution and 25 µg per lane as a starting point, then check performance in your own sample.
Source: BosterBio SUV39H1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O43463.
- Human Protein Atlas. SUV39H1 tissue expression.
- PMC12675686 — target-verified WB comparison
- PMC12065678 — target-verified WB comparison
- PMC5397975 — target-verified WB comparison