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- Table of Contents
SVOP / Synaptic vesicle 2-related protein · Western blot design guide
Design a Western Blot for SVOP
Source-linked SVOP Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SVOP WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled October 2026 · For research use; verify linked source records and product datasheet before use
SVOP Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~60.8 kDa | |
| Observed band | 72 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked SVOP Western Blot Protocol Options
The A15475-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | the lysates from Jurkat cells (catalog A15475-1) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A15475-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected SVOP Western Blot Band Size?
SVOP is predicted at 60.8 kDa, while an empirical band appears at 72 kDa; the cause of this difference is not established.
| Band near 72 kDa | Empirical SVOP band; confirm identity with peptide competition or other specificity controls |
| Band near 60.8 kDa | Near the predicted SVOP sequence mass; identity requires validation |
| Little or no band in soluble lysate | SVOP is a synaptic vesicle membrane protein and may be poorly recovered in the soluble fraction |
| Additional nearby bands | SVOP has three phosphoserine sites, but distinct phospho bands are not established |
💡Expected SVOP appearanceSVOP has a predicted mass of 60.8 kDa and an empirical band at 72 kDa; the cause of the difference is unknown, so confirm band identity with antibody specificity controls.
| Predicted SVOP mass | 60.8 kDa from sequence; apparent migration may differ |
| Phosphoserine Ser25 | Modification is documented; an apparent size effect is not established |
| Phosphoserine Ser31 | Modification is documented; an apparent size effect is not established |
| Phosphoserine Ser542 | Modification is documented; an apparent size effect is not established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Membrane associated SVOP may be poorly extracted | Check membrane solubilization and test a membrane enriched fraction |
| Band higher than expected | The empirical 72 kDa band exceeds the 60.8 kDa prediction; its cause is unknown | Verify identity with peptide competition and an independent antibody |
| Band lower than expected | Identity or sample integrity is uncertain | Check protease inhibition and verify the band with an independent antibody |
| Multiple bands | Phosphoserine sites exist, but distinct phospho bands are unproven | Use peptide competition or an independent antibody to identify specific bands |
| Weak or no signal | Membrane protein recovery may be low | Check extraction efficiency and enrich the membrane fraction |
| Fragments below expected size | Sample degradation is possible | Use protease inhibitors and compare freshly prepared lysate |
Experiment evidence
Western blot images showing SVOP band
A15475-1 · SVOP
A15475-1 · SVOP
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SVOP Western blot
🧪For positive controls for SVOP in Western blot, you can use no HPA-supported positive sample because no tissue or cell data are available.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA provides no tissue controls, and SVOP's membrane location may make detection in whole-cell lysate difficult.
HPA tissue expression evidence for SVOP
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced SVOP Western Blot Tips
Deeper troubleshooting and optimisation questions for SVOP, answered from its protein features.
How should SVOP band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could an SVOP isoform explain an additional band?
Isoforms · The supplied record lists one isoform and no alternative sequence. It provides no annotated isoform to assign to an additional band.
Which SVOP phosphorylation sites matter when interpreting bands?
PTM · UniProt lists phosphoserine at positions 25, 31, and 542, using its sequence numbering. Keep those coordinates when comparing site-specific results; antibody or paper numbering may differ. These sites alone do not establish a visible band shift or explain the 72 kDa band.
Does this guide establish induction of SVOP?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for SVOP Western blot?
Transfer · SVOP is annotated as a multi-pass synaptic-vesicle membrane protein. The supplied features do not establish a preferred transfer method. Check transfer efficiency when optimizing conditions, particularly around the observed 72 kDa band.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A15475-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should SVOP abundance be quantified on a blot?
Quantitation · Measure the SVOP signal within the assay’s linear range and use consistent membrane-protein extraction and loading across samples. The observed 72 kDa band is the empirical reference. Total-band intensity alone cannot distinguish changes in SVOP abundance from changes in phosphorylation at positions 25, 31, or 542.
Why might SVOP appear at 72 kDa instead of 60.8 kDa?
Interpretation · SVOP has a predicted mass of 60.8 kDa and an observed Western blot band at 72 kDa. Its multi-pass membrane annotation and phosphorylation sites do not establish the cause of that difference. Use the observed 72 kDa band as a reference, and avoid assigning the shift to a specific modification without further evidence.
Compare them with the observed 72 kDa band before assigning an identity. The record lists one isoform, no glycosylation sites, and no signal or propeptide cleavage feature. Phosphoserines at UniProt positions 25, 31, and 542 do not, by themselves, identify an unexpected band.
Compare them with the observed 72 kDa band before assigning an identity. The record lists one isoform, no glycosylation sites, and no signal or propeptide cleavage feature. Phosphoserines at UniProt positions 25, 31, and 542 do not, by themselves, identify an unexpected band.
Boster reagents
SVOP Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-SVOP Antibody
Cat # A15475-1
The catalog reports one anti-SVOP antibody, A15475-1, for Western blot with stated human, mouse, and rat reactivity. Its WB image shows Jurkat cell lysates; the supplied evidence does not show WB results for other sample types.
Which to pick: A15475-1 is the only listed option. Its WB image uses Jurkat cell lysates, so it is the directly illustrated choice for that context. Human, mouse, and rat reactivity is listed, but no other WB samples are shown.
Source: BosterBio SVOP gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q8N4V2.
- Human Protein Atlas. SVOP tissue expression.
- PMC2669215 — target-verified WB comparison