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- Table of Contents
SYNCRIP / Heterogeneous nuclear ribonucleoprotein Q · Western blot design guide
Design a Western Blot for SYNCRIP
Source-linked SYNCRIP Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SYNCRIP WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SYNCRIP Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~69.6 kDa | |
| Observed band | 64–74 kDa | |
| Gel | 10% (catalog M03946-1) | |
| Positive control | Caudate (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 5 isoform(s) |
Section 1
Source-Linked SYNCRIP Western Blot Protocol Options
The M03946-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Hela, human Jurkat, human HepG2, human MCF-7, rat lung, rat kidney, mouse lung, mouse kidney (catalog M03946-1) |
| Gel % | 10% (catalog M03946-1) |
| Load | 30 ug; reducing conditions (catalog M03946-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog M03946-1) |
| Membrane | nitrocellulose membrane (catalog M03946-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog M03946-1) |
| Primary antibody | M03946-1 · 1:1000 (catalog M03946-1) |
| Primary incubation | overnight at 4°C (catalog M03946-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog M03946-1) |
| Secondary incubation | 1.5 hour at RT (catalog M03946-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog M03946-1) |
| Detection | ECL (catalog M03946-1) |
Section 2
What Is the Expected SYNCRIP Western Blot Band Size?
SYNCRIP is predicted at 69.6 kDa and observed at 64–74 kDa; the cause of any difference is not established.
| Band at 64–74 kDa | Matches the empirical SYNCRIP band range. |
| Band near 69.6 kDa | Consistent with the predicted protein mass. |
| Several nearby bands | Could reflect isoforms 1–5 if their migration differs; band identity needs confirmation. |
| Slightly shifted band or doublet | Could reflect a modified form, including phosphorylation; a visible shift is not established. |
💡Expected SYNCRIP appearanceSYNCRIP has a predicted mass of 69.6 kDa and an empirical band at 64–74 kDa; confirm band identity with an appropriate antibody control or SYNCRIP depletion.
| Predicted SYNCRIP mass | 69.6 kDa predicted; the empirical band spans 64–74 kDa. |
| Isoform 1 | Its individual mass and migration are not supplied. |
| Isoform 2 | Its sequence may affect size, but its mass and migration are not supplied. |
| Isoform 3 | Its sequence may affect size, but its mass and migration are not supplied. |
| Isoform 4 | Its sequence may affect size, but its mass and migration are not supplied. |
| Isoform 5 | Its sequence may affect size, but its mass and migration are not supplied. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | SYNCRIP signal may be below detection in the sampled cells. | Check lysate loading and use a positive control lysate. |
| Band higher than expected | The listed modifications could affect migration, but a shift is not established. | Compare with the 64–74 kDa empirical range and confirm identity by SYNCRIP depletion. |
| Band lower than expected | An isoform or protein breakdown could contribute; neither has an assigned band size. | Confirm identity by SYNCRIP depletion and check sample handling. |
| Multiple bands | Five isoforms are listed, but distinct migration has not been demonstrated. | Use SYNCRIP depletion or an isoform-specific control to identify bands. |
| Weak or no signal | Insufficient detectable SYNCRIP or inefficient transfer. | Check loading, transfer, and a positive control lysate. |
| Fragments below expected size | Protein breakdown during sample preparation is possible. | Prepare fresh lysate with protease inhibitors and confirm band identity. |
Experiment evidence
Western blot images showing SYNCRIP band
M03946-1 · SYNCRIP
M03946-1 · SYNCRIP
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SYNCRIP Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for SYNCRIP in Western blot, you can use caudate tissue, which HPA rates as high expression.
Positive control: Caudate (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: Adipose tissue is HPA-not-detected, but confirm the negative result in your lysate.
HPA tissue expression evidence for SYNCRIP
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Caudate | glial cells | High | Protein (IHC) | HPA → |
| Cerebellum | cells in molecular layer | High | Protein (IHC) | HPA → |
| Cerebral cortex | glial cells | High | Protein (IHC) | HPA → |
| Hippocampus | glial cells | High | Protein (IHC) | HPA → |
| Ovary | follicle cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Low | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Low | Protein (IHC) | HPA → |
| Breast | glandular cells | Low | Protein (IHC) | HPA → |
| Lung | alveolar cells | Low | Protein (IHC) | HPA → |
Section 3
Advanced SYNCRIP Western Blot Tips
Deeper troubleshooting and optimisation questions for SYNCRIP, answered from its protein features.
What should be checked when unexpected SYNCRIP bands appear?
Band shift · Check the band against the 69.6 kDa predicted mass and reported 64–74 kDa apparent range, then review the five isoforms and antibody epitope. Annotated phosphorylation, acetylation, and PRMT1-mediated methylation are possible molecular differences, but their presence alone neither identifies an unexpected band nor proves a visible shift.
Could SYNCRIP isoforms produce different bands?
Isoforms · Yes. Five isoforms are listed. Relative to the canonical sequence, isoforms 2 and 4 lack residues 302–336; isoforms 3, 4, and 5 replace residues 550–623 with a shorter sequence. Consider isoform expression when assigning bands, but verify band identities experimentally.
Isoform 5 lacks canonical residues 1–152, replaces residues 153–163, and replaces residues 550–623 with a shorter sequence. An antibody recognizing a region absent from isoform 5 would not detect it. Check the antibody epitope before interpreting a missing or additional band.
Isoform 5 lacks canonical residues 1–152, replaces residues 153–163, and replaces residues 550–623 with a shorter sequence. An antibody recognizing a region absent from isoform 5 would not detect it. Check the antibody epitope before interpreting a missing or additional band.
Which phosphorylation sites should guide phospho-specific detection?
PTM · The supplied UniProt features list phosphoserine at canonical positions 159 and 587 and phosphotyrosine at 373. Match a phospho-specific antibody’s stated site and numbering convention to the relevant isoform; isoform 5 replaces the canonical sequence containing position 159.
Does this guide establish induction of SYNCRIP?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for SYNCRIP?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M03946-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should SYNCRIP bands be quantified across samples?
Quantitation · Define which bands enter the measurement and apply that choice consistently. SYNCRIP has five annotated isoforms and listed cytoplasmic, nuclear, microsomal, and endoplasmic-reticulum locations, so compare equivalent sample fractions and avoid assuming every detected band represents the same isoform.
How should the 64–74 kDa band compare with predicted mass?
Interpretation · SYNCRIP’s predicted mass is 69.6 kDa, within the reported 64–74 kDa apparent band range. The supplied features do not establish why any individual band migrates above or below 69.6 kDa.
UniProt lists PRMT1-mediated arginine methylation at canonical positions 444, 496, 510, 518, 526, 536, and 539. Positions 444, 518, 526, 536, and 539 have alternate mono- and asymmetric dimethylarginine annotations. These modifications alone do not establish a visible band shift.
UniProt lists PRMT1-mediated arginine methylation at canonical positions 444, 496, 510, 518, 526, 536, and 539. Positions 444, 518, 526, 536, and 539 have alternate mono- and asymmetric dimethylarginine annotations. These modifications alone do not establish a visible band shift.
Boster reagents
SYNCRIP Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-hnRNP Q Rabbit Monoclonal Antibody
Cat # M03946-1
The listed anti-SYNCRIP (hnRNP Q) rabbit monoclonal antibody, M03946-1, has a Western blot image showing a band at approximately 64–74 kDa (expected 70 kDa) in the reported human cell and rat and mouse tissue lysates. No publication evidence is supplied.
Which to pick: M03946-1 is the only listed option. It reports human, mouse, and rat reactivity and includes a WB image using HeLa, Jurkat, HepG2, MCF-7, and rat and mouse lung and kidney lysates at 1:1000. Compare those reported conditions with your experiment.
Source: BosterBio SYNCRIP gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O60506.
- Human Protein Atlas. SYNCRIP tissue expression.
- PMC13412200 — target-verified WB comparison
- PMC7025501 — target-verified WB comparison
- PMC5812486 — target-verified WB comparison