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- Table of Contents
TALDO1 / Transaldolase · Western blot design guide
Design a Western Blot for TALDO1
Source-linked TALDO1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-TALDO1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
TALDO1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~37.5 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked TALDO1 Western Blot Protocol Options
The A06530 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Target-positive lysate and matched negative control (standard starting point) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A06530; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected TALDO1 Western Blot Band Size?
TALDO1 has a predicted 37.5 kDa monomer; isoforms and homodimerization could affect band patterns, but distinct migration has not been demonstrated here.
| Band near 37.5 kDa | Consistent with the predicted TALDO1 monomer; confirm identity with antibody controls. |
| Band near 75 kDa | Could represent a homodimer if it persists during sample preparation. |
| Additional bands near the main band | Could reflect isoforms 1 and 2, though distinct migration is unestablished. |
| Weak band in an isolated nuclear or cytoplasmic fraction | Isoform 1 shuttles between these compartments. |
💡Expected TALDO1 appearanceThe predicted TALDO1 monomer is 37.5 kDa; no empirical band size is supplied, so confirm any candidate band with antibody and sample controls.
| Predicted monomer mass | Provides a 37.5 kDa reference, not a measured band position. |
| Homodimer formation | Could produce an approximately 75 kDa band if the dimer survives sample preparation. |
| Isoform 1 | May differ in size from isoform 2; the direction and magnitude are unspecified. |
| Isoform 2 | May differ in size from isoform 1; distinct migration is unestablished. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | TALDO1 is intracellular, so absent signal needs sample or antibody assessment. | Check a positive-control lysate, loading, and antibody performance. |
| Band higher than expected | A TALDO1 homodimer may persist during sample preparation. | Compare denaturing conditions and verify band identity. |
| Band lower than expected | An isoform or alternative initiation is possible, but its migration is unknown. | Check band identity with an independent antibody or TALDO1-depleted sample. |
| Multiple bands | Isoforms 1 and 2 are documented, but their band positions are unknown. | Use an independent antibody or TALDO1-depleted sample to identify specific bands. |
| Weak or no signal | Isoform 1 distributes between nucleus and cytoplasm. | Compare whole-cell lysate with nuclear and cytoplasmic fractions and check loading. |
Experiment evidence
Western blot images showing TALDO1 band
A06530 · TALDO1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TALDO1 Western blot
🧪For positive controls for TALDO1 in Western blot, you can use a validated TALDO1-positive lysate; the supplied HPA evidence identifies no tissue or cell line.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA expression data are unavailable, so tissue controls cannot be selected reliably.
HPA tissue expression evidence for TALDO1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced TALDO1 Western Blot Tips
Deeper troubleshooting and optimisation questions for TALDO1, answered from its protein features.
How should TALDO1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could TALDO1 isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2. Isoform 2 lacks residues 1–10 of isoform 1, so their sizes differ slightly; separate bands are not guaranteed. An antibody targeting those first ten residues would not detect isoform 2.
Which TALDO1 modifications matter when interpreting a blot?
PTM · UniProt lists N6-acetyllysine at positions 115, 219, 269, 286 and 321, and phosphoserine at positions 237 and 256. These are UniProt coordinates; check the numbering convention before comparing them with antibody documentation. Their presence does not establish a visible band shift.
Does this guide establish induction of TALDO1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for TALDO1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A06530 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should TALDO1 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
How should a TALDO1 band compare with its predicted mass?
Interpretation · The supplied predicted mass is 37.5 kDa, and no observed band size is available. Use 37.5 kDa as a reference, not an exact migration target. The listed modifications alone do not establish a visible shift or explain any difference from the predicted mass.
Check whether a nearby band could reflect isoform 2, which lacks residues 1–10. The listed acetylation and phosphorylation sites do not, by themselves, identify another band. No observed band size is supplied, so band identity needs experimental confirmation.
Keep the sample fraction consistent: isoform 1 is listed in both nucleus and cytoplasm and shuttles between them. For total TALDO1, use an antibody that recognizes sequence shared by both isoforms; an antibody against residues 1–10 of isoform 1 would miss isoform 2.
Yes. Isoform 1 is listed in both compartments and shuttles between them through importin alpha/beta dependent import and CRM1 export. Compare like fractions across samples when interpreting signal changes; a change in one fraction need not represent a change in total TALDO1.
Check whether a nearby band could reflect isoform 2, which lacks residues 1–10. The listed acetylation and phosphorylation sites do not, by themselves, identify another band. No observed band size is supplied, so band identity needs experimental confirmation.
Keep the sample fraction consistent: isoform 1 is listed in both nucleus and cytoplasm and shuttles between them. For total TALDO1, use an antibody that recognizes sequence shared by both isoforms; an antibody against residues 1–10 of isoform 1 would miss isoform 2.
Yes. Isoform 1 is listed in both compartments and shuttles between them through importin alpha/beta dependent import and CRM1 export. Compare like fractions across samples when interpreting signal changes; a change in one fraction need not represent a change in total TALDO1.
Boster reagents
TALDO1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Transaldolase TALDO1 Antibody
Cat # A06530
A06530 is a polyclonal anti-TALDO1 antibody listed for human and mouse reactivity. A Western blot image is available, but the supplied evidence does not specify the tested specimens, conditions, or band specificity.
Which to pick: A06530 is the only listed TALDO1 antibody. Consider it for human or mouse Western blots, and review its WB image and full product details for compatibility with your sample and conditions.
Source: BosterBio TALDO1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P37837.
- Human Protein Atlas. TALDO1 tissue expression.
- PMC12540763 — target-verified WB comparison
- PMC13458028 — target-verified WB comparison
- PMC11800534 — target-verified WB comparison