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- Table of Contents
Facts about Antigen peptide transporter 1.
Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding.
Human | |
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Gene Name: | TAP1 |
Uniprot: | Q03518 |
Entrez: | 6890 |
Belongs to: |
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ABC transporter superfamily |
ABC Transporter, MHC 1; ABC17; ABCB2; ABCB2FLJ41500; antigen peptide transporter 1; APT1; ATP-binding cassette sub-family B member 2; ATP-binding cassette, sub-family B (MDR/TAP), member 2; D6S114E; Ham1; Peptide supply factor 1; Peptide transporter involved in antigen processing 1; Peptide transporter PSF1; Peptide Transporter TAP1; PSF1; PSF-1; PSF1ABC17; Really interesting new gene 4 protein; RING4; RING4FLJ26666; TAP1; TAP1*0102N; TAP1N; transporter 1, ATP-binding cassette, sub-family B (MDR/TAP); transporter associated with antigen processing; transporter, ATP-binding cassette, major hist
Mass (kDA):
87.218 kDA
Human | |
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Location: | 6p21.32 |
Sequence: | 6; NC_000006.12 (32845209..32853971, complement) |
Endoplasmic reticulum membrane; Multi-pass membrane protein. The transmembrane segments seem to form a pore in the membrane.
Are you interested in learning more about Steven Boster Check out his bio to learn about his research in histology and biology. In this bio, you'll discover the histological roots and the significance of his work and how his work has impacted many fields of science. Boster has made many contributions to biology and his bio has been published in scientific journals all over the world. There are many applications for the TAP1 Marker. This article will focus on just a few of them.
Boster Bio's Historial background provides a background of the founder's scientific career. Steven Boster, known as "the man who converts science into a lavatory," invented his first product in 1993. He was the one responsible for the development of hundreds of primary antibodies, and eventually , China's most powerful catalog antibody manufacturer. Boster's firm eventually developed its own technology to offer high-sensitivity ELISA kits that are sold to scientists worldwide.
A variety of tissues have been studied to study the histological function of TAP1 gene. Recent research has discovered that the TAP1 gene could be able to identify the presence of a small portion of EC cases, despite its unclear role. It is important to know that TAP1 is a major contributor to the endometrium. To determine whether the TAP1 gene is involved in EC, the TAP1 gene was transduced into the lining of the endometrium of EC patients by using the BPCas9 and LacZ guide. TAP1 gene expression in B2Mcells was confirmed using flow cytometry.
It is important to note that this gene can perform numerous biological functions. Despite its widespread use in the detection of many different illnesses, it is also able to detect certain kinds of tumors. The most commonly known TAP1 target is the MUC5AC gene that is expressed by goblet secretory cells. The Proliferative subtype had the highest TAP1 expression which is more prevalent among smokers. The Proliferative subtype also saw an increase in SCGB1A1.
The TAP1 gene is thought to be a transcription factor that plays a key role in the immune response. It is controlled by a variety of signaling molecules like IFN-g and IL-10. The presence of poly(I.C) and LPS in pig tissues were used to test for TAP1 gene expression. The TAP1 marker gene was amplified in a cell line using an Pure Blood RNA Prep Kit from Tiangen Biotech in Beijing. To determine the expression of mRNA in the TAP1 gene Three pigs were chosen from the groups of control and infection.
The mutation was introduced into the TAP gene using an aquikchange site-directed Mutagenesis kit. Silent mutations were introduced in the TAP1 gene using primers 5'-CTTATCCTGAAGCCAGGAGCTCTTTTTAGA-TAP2E632-Q. The mutated DNAs were extracted from pPCR2.1 and ligated into the pAcUW51 vector. BaculoGold DNA was used to co-transfect insect cells.
Research has previously demonstrated that TAP1 gene variation is linked to immune-related characteristics in pigs. The gene is expressed in pig tissues including lymph nodes stomach, lungs, liver epididymis, fat, and stomach. It is not found in large amounts in the liver, small intestinales or muscles. This suggests that TAP1 could play a significant role in the immune response of swine.
TAP1 mutants with mutation D668N showed the same affinity for 8-azidoATP. In contrast, the mutants deficient in the TAP1 D668N subunit showed decreased translocation activity. This mutation did not cause significant differences in the binding of nucleotide agarose beads between mutants. TAP1 D668N also had significantly higher +atp/-atp ratios than wild type TAP-expressing microsomes.
Recent research has demonstrated that TAP1 and TAP2 levels are linked to the degree of tumor and HER2/neu status. These markers were also found to be associated with the expression levels of estrogen receptors and progesterone hormone receptors (PRs). These findings will help better understand the regulation of protein levels in tumor cells and could lead to improved regimens for immunotherapy. These promising applications will require further investigation however the TAP1 marker must be improved.
TAP1 is a fantastic candidate for vaccines and adjuvant immunotherapies. The TAP-containing vaccines will increase the antigens that are injected to cytotoxic CD8+ T cells. These treatments may not be appropriate for all types or subgroups of patients. TAP should be matched to specific cancer types and subgroups in order to avoid this problem.
These studies showed that TAP1 expression levels in grade 3 tumors were significantly higher than those in grade 1 and 2. The variation in TAP1 levels between stage 1 and 2 tumors was not statistically significant. These markers are not appropriate for use in clinical practice. They can be used to determine which cancerous cells are suitable for clinical trials. The researchers concluded that the combination of these two markers would be the most effective.
A study of PSMB9 and TAP1 genotypes in vitiligo demonstrated significant correlation with the development of the disease. It isn't conclusive. Further studies are needed to determine if they are linked to vitiligo. If these two markers are found as a risk factor, the risk of contracting the disease can increase from four to eightfold. This is a positive sign.
PMID: 2259383 by Trowsdale J., et al. Sequences encoded in the class II region of the MHC related to the 'ABC' superfamily of transporters.
PMID: 1453454 by Beck S., et al. DNA sequence analysis of 66 kb of the human MHC class II region encoding a cluster of genes for antigen processing.