Anti-Tap1 Antibody Picoband™

Product Name

Anti-Tap1 Antibody Picoband™

View all TAP1 Antibodies

SKU/Catalog Number

A01123-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Tap1 Antibody Picoband™ catalog # A01123-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Tap1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01123-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Tap1 recombinant protein (Position: E218-D724).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01123-2 is reactive to TAP1 in Mouse, Rat

Applications

A01123-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

87.218kDa

Background of TAP1

Transporter associated with antigen processing 1 (TAP1) is a protein that in humans is encoded by the TAP1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This protein forms a heterodimer with Tap2 that transports short peptides from the cytosol into the endoplasmic reticulum lumen. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Mouse
Flow Cytometry, 1-3 µg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Tap1 using anti-Tap1 antibody (A01123-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tap1 antigen affinity purified polyclonal antibody (Catalog # A01123-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tap1 at approximately 70 kDa. The expected band size for Tap1 is at 81 kDa.

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Figure 2. IF analysis of Tap1 using anti-Tap1 antibody (A01123-2).
Tap1 was detected in an immunocytochemical section of RAW264.7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Tap1 Antibody (A01123-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of HEPA1-6 cells using anti-Tap1 antibody (A01123-2).
Overlay histogram showing HEPA1-6 cells stained with A01123-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tap1 Antibody (A01123-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For TAP1 (Source: Uniprot.org, NCBI)

Alternative Names

ABC Transporter, MHC 1; ABC17; ABCB2; ABCB2FLJ41500; antigen peptide transporter 1; APT1; ATP-binding cassette sub-family B member 2; ATP-binding cassette, sub-family B (MDR/TAP), member 2; D6S114E; Ham1; Peptide supply factor 1; Peptide transporter involved in antigen processing 1; Peptide transporter PSF1; Peptide Transporter TAP1; PSF1; PSF-1; PSF1ABC17; Really interesting new gene 4 protein; RING4; RING4FLJ26666; TAP1; TAP1*0102N; TAP1N; transporter 1, ATP-binding cassette, sub-family B (MDR/TAP); transporter associated with antigen processing; transporter, ATP-binding cassette, major hist TAP1 ABC17, ABCB2, APT1, D6S114E, PSF-1, PSF1, RING4*0102N, TAP1N, TAP1 transporter 1, ATP binding cassette subfamily B member antigen peptide transporter 1|ABC transporter, MHC 1|ATP-binding cassette sub-family B member 2|ATP-binding cassette, sub-family B (MDR/TAP), member 2|peptide supply factor 1|peptide transporter PSF1|peptide transporter TAP1|peptide transporter involved in antigen processing 1|really interesting new gene 4 protein|transporter 1 ATP-binding cassette sub-family B|transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)|transporter associated with antigen processing|transporter, ATP-binding cassette, major histocompatibility complex, 1

For more info on TAP1, check out the TAP1 Infographic

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In this infographic, you will see the following information for TAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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