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- Table of Contents
Facts about Antigen peptide transporter 2.
Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP.
Human | |
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Gene Name: | TAP2 |
Uniprot: | Q03519 |
Entrez: | 6891 |
Belongs to: |
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ABC transporter superfamily |
ABC18; ABCB3; ABCB3ABC18; antigen peptide transporter 2; APT2; ATP-binding cassette, sub-family B (MDR/TAP), member 3; D6S217E; MHC 2; Peptide transporter involved in antigen processing 2; Peptide transporter PSF2; Peptide transporter TAP2; PSF2; PSF2PSF-2; Really interesting new gene 11 protein; RING11; RING11ATP-binding cassette sub-family B member 3; TAP2; transporter 2, ABC (ATP binding cassette); transporter 2, ATP-binding cassette, sub-family B (MDR/TAP); Y1
Mass (kDA):
75.664 kDA
Human | |
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Location: | 6p21.32 |
Sequence: | 6; NC_000006.12 (32821833..32838770, complement) |
Endoplasmic reticulum membrane; Multi-pass membrane protein. The transmembrane segments seem to form a pore in the membrane.
The TAP2 Marker is a crucial biomarker that is involved in ER degradation as well as cell proliferation and tumorigenesis. It is a protein that has high specificity and affinity. Boster's antibodies have been validated on various platforms and with known positive and negative samples. They are rewarded to scientists who are the first to review their products and provide product credit. This program is open to all scientists around the world.
There are a number of applications for the marker TAP2. The fluorescent protein is easily available for use in cell-based immunoassays. It is useful in the detection of viral inhibitors. Here are a few of the most beneficial uses for the TAP2 marker. Read on to discover more. This article will provide a review of some of the most promising uses of this fluorescent protein. The applications will be detailed in this article.
This fluorescent protein is also utilized in cell-based research to identify the cellular proteins that are involved in the BoHV-1 UL49.5-induced degradation. It is assumed that this ATPase is degraded via the endoplasmic-reticulum related degradation pathways. The UL49.5 inhibitor, NMS-873 blocks the enzyme p97/VCP, which is involved in the degrading of the TAP2 protein.
TAP2 was genetically separated from TMD0 to isolate it from the ER. The TAP2 protein was later located in the ERGIC and ER-Golgi intermediate compartments. The marker is not used to display antigens. It is utilized in a variety of kinds of experiments, such as immunoassays based on cells.
Boster Bio: The Best Uses for the GFP-TAP2 Marker
Boster Bio: The first step to develop innovative antivirals is to utilize the TAP2 marker in cancer cells. In this study, researchers employed the fluorescent TAP platform to target the cellular proteins involved in BoHV-1 UL49.5 activity. TAP is an ER-resident cell protein and its degradation is thought to be through endoplasmic reticulum associated degradation pathways. This research also suggests that p97 plays a crucial role in UL49.5-mediated degradation.
Boster Bio Anti-CD8 Alpha/Cd8a Monoclonal Antibody is FITC conjugated, flow-tested and flow-tested. It reacts with Human. This antibody is stable in an ice-cold refrigerator at -20degC, or 4degC for a period of up to one month. It contains Trehalose, recombinant proteins and It can be used in studies that require quantitative data and are applicable to all scientists.
The position of the tag determines the intensity of fluorescence. TAP2 cells show greater fluorescence emanating from the N-terminus than the C-terminal. TAP1 has both ends in the cytoplasm while TAP2 has its N-terminus located in the ER lumen. BoHV-1's UL49.5 protein is not the principal target for the TAP2 Marker.
The TAP2 Marker, a highly conserved protein, is found both on the outside and inside of cell membranes. This protein is essential for the transport of a range of proteins, lipids and ions. The expression of this protein is significantly decreased in cancerous cells, and its presence is a significant indicator for the development of anti-cancer drugs. The TAP2 Marker is used in a variety of ways.
TAP1 and TAP2 are membrane proteins with the potential to form dimers. TAP1 and TAP2 don't have signals that can be cleaved but they could interact with ER translocation machinery. Its first TMS could also serve as a "stop-transfer" sequence. Many types of membrane proteins can be synthesized with the N termini of cytoplasm and the C termini outside of the cell.
TAP2 reduces MMP13 and the proinflammatory cytokines gene expression. In humans, TAP2 inhibits the expression of TLR4 in synoviocytes. Furthermore, TAP2 lowers the production of ROS in cartilage with arthritis. It can also be used to treat and diagnose inflammation-related illnesses. The clinical applications of it are endless.
This marker is most effective to detect changes in TAP1 or TAP2. These genes are frequently expressed in cancers as well as different tissues. Researchers must clone the correct DNA to identify these genes. These cDNAs have high homology with TAP1, TAP2, and HAF-4. Cloning is simple and only requires a primer pair to amplify the TAP gene.
After cloning these constructs, we performed Western Blotting and IP tests. The TAP2 and HAP1 fusion proteins showed similar levels of expression and co-immunoprecipitation with TAP1. The N-TG or eNTG constructs showed no significant binding to TAP. The ICP47-TG construct however, bound TAP with a similar strength.
The TAP2-GFP marker is numerous potential applications. It can be used to detect antibodies that are extracellular in cells , and to assess the effects of various treatments or drugs. It is particularly useful in studies of cancer as well as other diseases. It can also be used for screening for tumor-derived cells. The TAP2-GFP marker's value isn't only useful for the detection of tumor markers, but also used to identify inflammatory disease markers such as tumefa vaccination.
The TAP2-GFP half-type ATP binding cassette transporter that is able to bind ATP. ATP is essential to enable peptide transport in a unidirectional manner. It also requires coordinated structural rearrangements between NBDs and TMDs. The TAP2-GFP gene is highly conserved between different species. Its homologous genes TAP1 (TAP2) have 33% of their sequence homology. However, they have different roles in the translocation cycle.
PMID: 1453454 by Beck S., et al. DNA sequence analysis of 66 kb of the human MHC class II region encoding a cluster of genes for antigen processing.
PMID: 1741401 by Powis S.H., et al. Polymorphism in a second ABC transporter gene located within the class II region of the human major histocompatibility complex.