Product Info Summary
SKU: | PB9824 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-TAP2 Antibody Picoband™
SKU/Catalog Number
PB9824
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TAP2 Antibody Picoband™ catalog # PB9824. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9824)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TAP2, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9824 is reactive to TAP2 in Human
Applications
PB9824 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
87 kDa
Calculated molecular weight
75.664kDa
Background of TAP2
Transporter, ATP-binding cassette, major histocompatibility complex 2 (TAP2) is a gene in humans that encodes the protein Antigen peptide transporter 2. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. The gene is assigned to human chromosome 6p21.3. It is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. And this protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TAP2 using anti-TAP2 antibody (PB9824).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAP2 antigen affinity purified polyclonal antibody (Catalog # PB9824) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAP2 at approximately 87 kDa. The expected band size for TAP2 is at 87 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TAP2 (Source: Uniprot.org, NCBI)
Gene Name
TAP2
Full Name
Antigen peptide transporter 2
Weight
75.664kDa
Superfamily
ABC transporter superfamily
Alternative Names
ABC18; ABCB3; ABCB3ABC18; antigen peptide transporter 2; APT2; ATP-binding cassette, sub-family B (MDR/TAP), member 3; D6S217E; MHC 2; Peptide transporter involved in antigen processing 2; Peptide transporter PSF2; Peptide transporter TAP2; PSF2; PSF2PSF-2; Really interesting new gene 11 protein; RING11; RING11ATP-binding cassette sub-family B member 3; TAP2; transporter 2, ABC (ATP binding cassette); transporter 2, ATP-binding cassette, sub-family B (MDR/TAP); Y1 TAP2 ABC18, ABCB3, APT2, D6S217E, PSF-2, PSF2, RING11 transporter 2, ATP binding cassette subfamily B member antigen peptide transporter 2|ABC transporter, MHC 2|ATP-binding cassette, sub-family B (MDR/TAP), member 3|peptide supply factor 2|peptide transporter PSF2|peptide transporter involved in antigen processing 2|really interesting new gene 11 protein|transporter 2, ABC (ATP binding cassette)|transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAP2, check out the TAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAP2 Antibody Picoband™ (PB9824)
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Customer Q&As
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1 Customer Q&As for Anti-TAP2 Antibody Picoband™
Question
We are currently using anti-TAP2 antibody PB9824 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-TAP2 antibody (PB9824) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08