This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
TEP1 / Telomerase protein component 1 · Western blot design guide
Design a Western Blot for TEP1
Source-linked TEP1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-TEP1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
TEP1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~290.5 kDa | |
| Gel | 4–12% gradient (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked TEP1 Western Blot Protocol Options
The A02017 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human kidney tissue lysate (catalog A02017) |
| Gel % | 4–12% gradient (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Wet/tank transfer; optimize duration (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A02017 · (A) 0.5 and (B) 1 μg/mL (catalog A02017) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected TEP1 Western Blot Band Size?
TEP1 is predicted at 290.5 kDa; alternative splicing may affect size, but no empirical migration or distinct isoform bands are demonstrated.
| Band near 290.5 kDa | Consistent with the predicted TEP1 mass; confirm identity with controls |
| Single band at another size | Migration alone cannot establish TEP1 identity or an isoform assignment |
| Two bands at different sizes | Isoforms 1 and 2 exist, but their migration difference is unknown |
| Band enriched in a nuclear fraction | Consistent with TEP1's nuclear location; confirm identity with controls |
💡Expected TEP1 appearanceTEP1 has a predicted mass of 290.5 kDa, but no empirical band size is supplied; use appropriate band-identity controls to assess any detected band.
| Predicted TEP1 mass | Provides a 290.5 kDa reference, not a measured migration position |
| Isoform 1 | Its separate mass and migration are not supplied |
| Isoform 2 | Its separate mass and migration are not supplied |
| Alternative splicing | May affect protein size, but distinct bands are not established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Nuclear TEP1 may be difficult to detect in whole-cell lysate | Check a nuclear fraction and a validated positive control |
| Band higher than expected | The band cannot be assigned to TEP1 by size alone | Check antibody specificity with a TEP1-depleted sample |
| Band lower than expected | An isoform assignment cannot be made without isoform sizes | Compare with a TEP1-depleted sample and an isoform-specific control if available |
| Multiple bands | Alternative splicing is documented, but distinct isoform bands are not established | Identify TEP1-dependent bands with a depletion control |
| Weak or no signal | The nuclear target may be diluted in whole-cell lysate | Enrich the nuclear fraction and check a validated positive control |
Experiment evidence
Western blot images showing TEP1 band
A02017 · TEP1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TEP1 Western blot
🧪For positive controls for TEP1 in Western blot, you can use no HPA-supported tissue or cell line because no positive candidates were supplied.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA data are unavailable, so tissue controls cannot be selected or validated from the supplied evidence.
HPA tissue expression evidence for TEP1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced TEP1 Western Blot Tips
Deeper troubleshooting and optimisation questions for TEP1, answered from its protein features.
How should TEP1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Can TEP1 isoforms produce different bands?
Isoforms · UniProt lists two isoforms. In isoform 2, a 32-residue segment at canonical positions 2475–2506 is replaced by a 25-residue segment. This sequence difference does not establish that the isoforms resolve as separate bands.
Examine the altered sequence at positions 2475–2506 in UniProt canonical numbering. An antibody against a sequence shared by both isoforms cannot distinguish them; an antibody targeting the isoform-specific sequence may. Check the antibody's stated numbering convention before comparing coordinates.
Examine the altered sequence at positions 2475–2506 in UniProt canonical numbering. An antibody against a sequence shared by both isoforms cannot distinguish them; an antibody targeting the isoform-specific sequence may. Check the antibody's stated numbering convention before comparing coordinates.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of TEP1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for TEP1 Western blot?
Transfer · TEP1 is predicted to be 290.5 kDa. Choose a transfer setup suitable for a protein of that size, then check transfer with a high-mass marker and a total-protein stain. The supplied features do not specify a membrane, transfer time, or voltage.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A02017 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should TEP1 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Should TEP1 run at its predicted 290.5 kDa?
Interpretation · 290.5 kDa is the predicted mass; no observed band size is supplied. Use a high-mass marker to assess migration, and do not treat a difference from 290.5 kDa alone as evidence of a modification.
Keep sample preparation consistent and compare signals within the assay's linear range. State whether samples are whole-cell or enriched fractions: UniProt places TEP1 in the nucleus and at telomeres and lists it as a component of telomerase and vault particles.
Check the band against the predicted 290.5 kDa mass and the antibody's recognized sequence. The two annotated isoforms differ near the C-terminus, but their existence alone does not identify an unexpected band. No signal peptide, propeptide, modified residue, or glycosylation site is listed to support a specific processing or modification explanation.
Keep sample preparation consistent and compare signals within the assay's linear range. State whether samples are whole-cell or enriched fractions: UniProt places TEP1 in the nucleus and at telomeres and lists it as a component of telomerase and vault particles.
Check the band against the predicted 290.5 kDa mass and the antibody's recognized sequence. The two annotated isoforms differ near the C-terminus, but their existence alone does not identify an unexpected band. No signal peptide, propeptide, modified residue, or glycosylation site is listed to support a specific processing or modification explanation.
Boster reagents
TEP1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-TP1 TEP1 Antibody
Cat # A02017
the supplier A02017 is an anti-TEP1 antibody listed for human, mouse, and rat. Its Western blot image shows human kidney tissue lysate tested at 0.5 and 1 μg/mL; the supplied evidence does not show mouse or rat blots.
Which to pick: A02017 is the only listed option. Its Western blot image supports use in the reported human kidney lysate conditions; mouse and rat reactivity is listed but not shown in the supplied image.
Source: BosterBio TEP1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q99973.
- Human Protein Atlas. TEP1 tissue expression.
- PMC12217205 — target-verified WB comparison