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Facts about Tight junction protein ZO-1.
Necessary for lumenogenesis, and especially efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB into the leading edge of migrating cells (PubMed:21240187).
Human | |
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Gene Name: | TJP1 |
Uniprot: | Q07157 |
Entrez: | 7082 |
Belongs to: |
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MAGUK family |
DKFZp686M05161; MGC133289; tight junction protein 1 (zona occludens 1); Tight junction protein 1; tight junction protein ZO-1; TJP1; ZO1; ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Mass (kDA):
195.459 kDA
Human | |
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Location: | 15q13.1 |
Sequence: | 15; NC_000015.10 (29699367..29969049, complement) |
The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Cell projection, podosome. Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact (PubMed:7798316). At podosomal sites, is predominantly localized in the ring structure surrounding the actin core (PubMed:20930113).
If you're considering buying Boster Bio's Anti-ZO1 tight junction protein/TJP1 marker You may be wondering what are the best methods to make the most of the experiment. This guide will provide strategies for optimizing your research to achieve the best results. There's a good chance that you've encountered issues with one aspect of your research as a researcher. While there are a variety of possible causes of error the proper controls can help eliminate them. These troubleshooting tips will help you identify and eliminate the causes of errors in the Boster Bio system.
Boster Bio's Anti-ZO1/TJP1 marker has been created to identify ZO1 (tight junction proteins) in a variety cell types. The TJP1 gene encodes a protein that is located on the cytoplasmic side of intercellular tight junctions. The encoded protein could play the role of signal transduction at cell-cell junctions. Two distinct transcript variants are also present in the protein. the N-terminal encodes an enzyme that may be involved with tight junction assembly, while the C-terminal has specific properties for the tight junction. The alpha domain, which is believed to be involved in stabilizing junctions, could also play a part.
ZO-1 is a membrane protein that is found on the cytoplasmic surface of most cells. It plays a key role in regulating paracellular flow and also in influencing apical-basal and polarity. ZO-1 can be found in a variety of tissues. It interacts with proteins in liver, kidney, and skeletal muscles cells. This protein is an essential component of cell membranes, tight junctions, and cell membranes.
The detection limit for TJP1 was approximately 0.25ng/lane under reducing conditions but may be slightly less for other applications. Additionally the product has been tested on domestic species. The optimum dilutions for staining formalin/paraffin sections have not yet been established. However, dilutions can differ, and the stain should be tested for all applications.
The anti-ZO1 tight joint protein/TJC1 marker can be used to detect the tight junction protein ZO1. The TJP1 anti-ZO1 is present in both human and murine samples. It has been demonstrated to be effective in identifying a wide range of cell types and their distinct functions. Boster Bio's Anti ZO1 TJP1 Assay can assist you in determining the best anti ZO1 TJP1 or protein marker for ZO1 detection in cells in culture.
The anti-ZO1 antibody is a specific TJP1 marker that is able to detect ZO-1 within a variety of tissues including colon duodenum, rectum, cecum, and pancreas. It targets ZO-1 within the intestinal wall which plays a crucial role in the regulation and the proliferation of cells.
The anti-ZO1 antibody recognizes a specific peptide that is a tight junction protein known as zonula Occluns. This protein is an important part of the intestinal wall. It is responsible to ensure that the tissue can be properly sealed and maintain an intestinal barrier to prevent gastric lesion. Anti-ZO1 antibody, which is a specific and high-resolution immune staining antibody, is made to detect the protein that causes gastric lesions.
The TJP1 gene, also known as zona occludens 1 regulates actin cytoskeleton remodeling. Numerous cancers have altered TJP1 expression. These changes may impact the growth and invasion of cancer. Research has examined the TJP family in cancer cell lines and as a biomarker of bladder cancer staging. We will now discuss the advantages and drawbacks of the TJP1 gene marker.
In addition to its role in determining the presence or absence of disease, the TJP1 gene is also involved in the development of cancer and inflammation. These proteins are vital in the process of signaling between cells. TJP1 markers are useful in identifying cancerous cells as well as other types. It is also used to detect illnesses caused by the deregulation of junctional signaling pathways.
The intestinal barrier's physical structure is vital for digestion of nutrients and the integration of immunity. Numerous inflammatory and metabolic disorders may affect the integrity of TJ. It is therefore beneficial to boost TJ activity in patients with various diseases. Researchers in the pharmaceutical industry are investigating the nutritional factors that can improve TJ activity. These substances have the potential to improve TJ functionality and integrity and thus prevent or treat diseases. Recent research has proven that the TJP1 gene plays a vital role in tight junction assembly.
TJP1 gene expression was up in airway epithelial cells and BECs in comparison to healthy BECs. The mRNA Ct values for both genes were not significantly different between healthy and asthmatic patients. This suggests that TJP1 gene expression could be useful for predicting airway remodeling. The gene is also linked to differentiation of the epithelium in the airway.
The TJP1 gene has been shown to be a biomarker that can be useful in bladder cancer. TJP1 protein expression is correlated with the stage of tumor and its the grade. The gene can be used as a biomarker to determine bladder cancer stage. TJP1 gene expression can be studied in 40 different types of cancer cell lines in the Cell Line Encyclopedia database. In addition, the gene copy number of TJP1 is evaluated in a variety of cancer cell lines.
PMID: 8395056 by Willott E., et al. The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions.
PMID: 7798316 by Furuse M., et al. Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions.
*Showing only the more recent 20. More publications can be found for each product on its corresponding product page