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TJP1 antibody (PB9234) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 85 Publications

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IHC analysis of TJP1 using anti-TJP1 antibody (PB9234).
TJP1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TJP1 Antibody (PB9234) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-ZO1 tight junction protein/TJP1 Antibody Picoband®

Name: Anti-ZO1 tight junction protein/TJP1 Antibody Picoband®
Brand: Boster Bio
SKU: PB9234
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

TJP1 antibody

85 Publications|

2 Reviews

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Reactive Species	Host	Application
Human, Monkey, Mouse, Rat	Rabbit	IP, IF, IHC, ICC, WB

Tested Applications

TJP1 antibody was positively detected by WB in:

human HepG2 whole cell, human 293T whole cell, human U2OS whole cell, African green monkey COS-7 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH3T3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

TJP1 antibody was positively detected by IHC in:

human kidney tissue, human testis tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

TJP1 antibody was positively detected by ICC/IF in:

A431 cell

TJP1 antibody was positively detected by IP in:

U2OS cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Mouse

Tested

Monkey

Predicted

Product Information

Product Name

Anti-ZO1 tight junction protein/TJP1 Antibody Picoband®
View all TJP1 Antibodies

Form

Lyophilized

Uniprot

Q07157

Gene ID

7082

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TJP1 recombinant protein (Position: H1178-F1527). Human TJP1 shares 82% amino acid (aa) sequence identity with mouse TJP1.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

220-250 kDa

Calculated Molecular Weight

195.5 kDa

Background of TJP1

Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene. It is mapped to 15q13.1. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell–cell junctions. It has been found that injected CagA associates with the epithelial tight-junction scaffolding protein TJP1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex.

Cite This Product

Anti-ZO1 tight junction protein/TJP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9234)

Protocols

Western Blot protocol for TJP1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for TJP1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TJP1 antibody

Review Protocol

Immunoprecipitation (IP) protocol for TJP1 antibody

Review Protocol

Publications

Guchang capsule alleviates inflammatory bowel disease by downregulating lncRNA THRIL and inhibiting the p38 MAPK/NF-κB signaling pathway

Published: 2026-08-11

Journal: MOLECULAR IMMUNOLOGY

Application: WB

Impact Factor: 3.70

Species: Human

PMID: 42579965

SKU	PB9234
Application	Immunofluorescence
Sample	human Caco-2 cell line
Sample Processing Description	Cells were normally cultured in 24-well plates using MEM supplemented with 20% fetal bovine serum. When the cell density reached approximately 60%, the culture was terminated. The medium was removed, cells were washed three times with PBS, fixed with 4% paraformaldehyde for 15 minutes, and then washed three times with PBS before further use.
Other Reagents	Goat serum, DAPI, and an anti-fade mounting medium.
Primary Antibody	ZO1 tight junction protein/TJP1 Antibody Picoband®
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation	45 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	Caco-2 cells are a “gold standard” in vitro model for studying intestinal epithelial function, and ZO-1 is a key marker of barrier integrity. The images show immunofluorescence staining of ZO-1 in normally cultured Caco-2 cells to evaluate antibody performance. Clear membrane localization and accurate staining indicate that this antibody is suitable for downstream research applications.

SKU	PB9234
Application	ICC/IF
Sample	Endothelial cells
Sample Processing Description	Endothelial cells were seeded in collagen gel-treated chips for 12 hours.
Primary Antibody	Anti-ZO1 tight junction protein/TJP1 Antibody Picoband®
Primary Incubation	1:200, overnight at 4 ℃
Blocking Agent	Ready-to-use Goat Serum
Secondary Antibody	Goat Anti-Rabbit IgG (H+L) Secondary Antibody, DyLight®488 Conjugated(BA1127)
Secondary Incubation	Incubate at room temperature for 1 hour
Other reagents	DAPI (AR1176)， Anti-fade mounting medium
Detection	Fluorescence microscope
Results Summary	At a critical stage of our manuscript preparation, we needed to stain tight junction proteins in endothelial cells. After trying primary antibodies from many companies without achieving satisfactory results, it was ultimately Boster’s product that solved the problem (Figure 5A). I was so excited that I took the photo below — treating it like a true treasure.

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Anti-ZO1 tight junction protein/TJP1 Antibody Picoband®

TJP1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Guchang capsule alleviates inflammatory bowel disease by downregulating lncRNA THRIL and inhibiting the p38 MAPK/NF-κB signaling pathway

Multiomics Integration Reveals AFB1 Causes Liver Damage Involving the Gut–Microbiota–Lipid Metabolism Axis in Piglet

Betulinic Acid Enema Alleviates Colitis by Targeting Vitamin D Receptor to Protect the Intestinal Mucosal Barrier and Modulate Mucosa-Associated Microbiota

The role and mechanism of TNFRSF21 in promoting necroptosis of vascular endothelial cells and inducing vascular leakage in sepsis

Olink Proteomics Reveals CCL2 Aggravates Perihematomal Edema After Intracerebral Hemorrhage in High-Altitude Migrants Via CCR2/NF-κB-Mediated Blood–Brain Barrier Disruption

ACT001 alleviates sepsis-induced acute lung injury by downregulating PANoptosis via the JAK2/STAT3 pathway

Resveratrol Attenuates Neuroinflammation and Gut–Brain Axis Dysfunction Induced by Lead and Cadmium Co-Exposure by Modulating Gut Microbiota in Rats

Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens

Microfluidic Construction of Glioma Micromodels on Hydrogel Microspheres for Drug Testing

Microglial P2RY12 mediates migration to and protection of cerebral microvasculature after ischemia–reperfusion via Caveolin-1

Discovery of Myristic acid, from chick early amniotic fluid for the treatment of acute gastric ulcers by targeting pyruvate carboxylase to improve TCA cycle

Revealing the Immune and Inflammatory Mechanisms of Electroacupuncture in Male IBS Rats Through Multi-Omics Analysis

Electroacupuncture Prevents Against AD‐Like Phenotypes in APP/PS1 Mice: Investigation of the Mechanisms From Cerebral Microangiopathy

Medulla Tetrapanacis Water Extract Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis by Reprogramming Macrophage Polarization and Maintaining Intestinal Barrier Integrity

Stretchable Sensor-Integrated Microfluidic Chip for Monitoring Fatty Acid-Enhanced Serotonin Release from the Intestinal Epithelium

β-Nicotinamide mononucleotide alleviates Alcohol-Induced liver injury in a mouse model through activation of NAD+/SIRT1 signaling pathways

Antioxidant-Targeting Synergistic Probiotic Delivery System with Improved Probiotics Viability and Intestinal Retention for Ulcerative Colitis Therapy

Investigating the protective role of Astragalus polysaccharides against fluoride-induced testicular injury via network pharmacology, molecular docking, and in vivo experiments

Douchi-Derived Bacillus subtilis Modulates Gut Microbiota and Bile Acid Metabolism to Alleviate Metformin-Induced Diarrhea in Type 2 Diabetes Mellitus

Arabinoxylan from barley bran alleviates ulcerative colitis in mice through enhancement of mucosal barrier function and modulation of gut microbiota

An orally-administered nanotherapeutics with gold nanospheres supplying for rheumatoid arthritis therapy by re-shaping gut microbial tryptophan metabolism

Alleviation Effect of the Secondary Metabolite of Anthocyanin (Protocatechuic Acid) on Heterocyclic Amine (IQ)-Induced Liver Injury and Its Underlying Mechanism

Therapeutic role of Prunella vulgaris L. polysaccharides in non-alcoholic steatohepatitis and gut dysbiosis

LncRNA FOXD3-AS1 modulates ER stress and epithelial barrier dysfunction in allergic rhinitis by destabilizing CHOP mRNA

Using gut microbiota and non-targeted metabolomics techniques to study the effect of xylitol on alleviating DSS-induced inflammatory bowel disease in mice

Hypoxia inducible factor-1alpha expression correlates with inflammatory injury of blood-brain barrier which influences perihaematomal edema after intracerebral hemorrhage

Evaluation of the protective role of resveratrol on LPS-induced septic intestinal barrier function via TLR4/MyD88/NF-κB signaling pathways

Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor Pathway

Transcranial alternating current stimulation inhibits ferroptosis and promotes functional recovery in spinal cord injury via the cGMP-PKG signalling pathway

Shexiang Tongxin dropping pills ameliorate myocardial ischemia-reperfusion injury progression via the S1PR2/RhoA/ROCK pathway

osa-miR168a, a Plant miRNA That Survives the Process of In Vivo Food Digestion, Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice by Oral Administration

Engineered Hybrid Lantibiotic that Selectively Combats Infections Caused by Staphylococcus aureus

Cornus Officinalis Total Glycosides Alleviate Granulomatous Lobular Mastitis via the B7-CD28/CTLA-4 Costimulatory Pathway

Investigating the modulatory effects of lactoferrin on depressed rats through 16S rDNA gene sequencing and LC–MS metabolomics analysis

Concurrent ozone and high temperature exacerbates nasal epithelial barrier damage in allergic rhinitis mice: Insights from the nasal transcriptome and nasal microbiota

Coke oven emissions exacerbate allergic asthma by promoting ferroptosis in airway epithelial cells

Fermented licorice extract alleviates ulcerative colitis by inhibiting the TLR4/NF-κB pathway and rebuilding intestinal microbiota in mice

A neurotherapeutic approach with Lacticaseibacillus rhamnosus E9 on gut microbiota and intestinal barrier in MPTP-induced mouse model of Parkinson’s disease

Lactobacillus plantarum-Derived Extracellular Vesicles Modulate Macrophage Polarization and Gut Homeostasis for Alleviating Ulcerative Colitis

Peripheral cytokine interleukin-10 alleviates perihematomal edema after intracerebral hemorrhage via interleukin-10 receptor/JAK1/STAT3 signaling

Fabrication of sac-like hydrogel membranes for replicating curved tissue barriers on chips

Prunella vulgaris L. attenuates gut dysbiosis and endotoxin leakage against alcoholic liver disease

Babao Dan alleviates gut immune and microbiota disorders while impacting the TLR4/MyD88/NF-кB pathway to attenuate 5-Fluorouracil-induced intestinal injury

Effects of Neuroinflammation and Autophagy on the Structure of the Blood–Brain Barrier in ADHD Model

Hydroxyfasudil regulates immune balance and suppresses inflammatory responses in the treatment of experimental autoimmune encephalomyelitis

The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice

Shexiang Tongxin Dropping Pill alleviates M1 macrophage polarization-induced inflammation and endothelial dysfunction to reduce coronary microvascular dysfunction via the Dectin-1/Syk/IRF5 pathway

Probiotics-loaded microcapsules from gas-assisted microfluidics for inflammatory bowel disease treatment

Hybrid stem cell-derived bioresponsive vesicles for effective inflamed blood-brain barrier targeting delivery

Probiotics-loaded carrageenan microspheres for inflammatory bowel disease treatment

Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A

Evaluation of two laboratory model methods for diarrheal irritable bowel syndrome

Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota

Interaction of Companilactobacillus crustorum MN047-derived bacteriocins with gut microbiota

Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia

Midazolam Ameliorates Impairment of the Blood–Brain Barrier (BBB) Against LPS

Three important short-chain fatty acids (SCFAs) attenuate the inflammatory response induced by 5-FU and maintain the integrity of intestinal mucosal tight junction

Intestinal epithelial cell-derived exosomes package microRNA-23a-3p alleviate gut damage after ischemia/reperfusion via targeting MAP4K4

Protective Effects of Companilactobacillus crustorum MN047 against Dextran Sulfate Sodium-Induced Ulcerative Colitis: A Fecal Microbiota Transplantation Study

Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora

Piperazine ferulate prevents high‑glucose‑induced filtration barrier injury of glomerular endothelial cells

Genetically encoded probiotic EcN 1917 alleviates alcohol-induced acute liver injury and restore gut microbiota homeostasis

The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis

Delivering Antisense Oligonucleotides across the Blood-Brain Barrier by Tumor Cell-Derived Small Apoptotic Bodies

Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora

Genistein ameliorates inflammation and insulin resistance through mediation of gut microbiota composition in type 2 diabetic mice

Hydrogel microfluidic-based liver-on-a-chip: Mimicking the mass transfer and structural features of liver