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- Table of Contents
Facts about Serine/threonine-protein kinase tousled-like 2.
Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy.
Human | |
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Gene Name: | TLK2 |
Uniprot: | Q86UE8 |
Entrez: | 11011 |
Belongs to: |
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protein kinase superfamily |
EC 2.7.11; EC 2.7.11.1; MGC44450; PKU-ALPHA; serine/threonine-protein kinase tousled-like 2; tousled-like kinase 2serine/threonine kinase
Mass (kDA):
87.661 kDA
Human | |
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Location: | 17q23.2 |
Sequence: | 17; NC_000017.11 (62470908..62615481) |
Ubiquitous. Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes.
Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2.
Biochemical assays are typically performed using a monoclonal or a polyclonal antibody to detect TLK2 in a variety animals. Boster Bio has used rabbit and mouse cells for the development of the TLK2 antigen. The Serine/Threonine Protein Kinase (SLK) is a crucial chromatin-assembly factor that phosphorylates ASF1A to prevent its degradation by the proteasome and enhances chromatin assembly.
Boster Bio's ELISA & antibodies kits were developed with scientists from many fields. They have been rigorously validated by international researchers. They have received over 29,000 publications. Boster Bio offers antibodies to human and mouse samples. It also offers a free secondary antibody when you buy a primary antibody. No matter which technique you use to make your product, you can be assured that it will work as promised.
Primary antibodies are immunoglobulins that attach to a specific antigen. Their affinity measures the strength of their noncovalent bonds. They can also be less specific if they bind antigens not intended for them. Good primary antibodies are generally able to detect, purify, or measure a specific type of antigen.
Secondary antibodies are often associated with the same antigen. This allows researchers to ask more specific questions about a target. Dual labeling allows researchers access to more context information and robust answers. These secondary antibodies are useful in multiple immunoassays. They can also be used in ELISAs. These antibodies are very useful in many types and types of research. Below is a list of Boster's high affinity primary antibodies for TLK2 markers.
Fluorescent MIHC can also be used with multiple markers. Fluorescent IHC is often used in combination with fluorescent primary antibodies. This is especially true when the latter is combined with dye-conjugated, secondary antibodies. Fluorescent mIHC has a problem in that there are only three marker filter sets. Hence, it is crucial to select primary antibodies that were raised in different species, isotypes, and/or concentrations.
High-affinity antibodies need to have a high affinity for the epitope found in fixed tissue. This affinity constant might not be known. TLK2's affinity constant is approximately 1.2. It is likely that the TLK2 epitope is present in the fixed tissue. It is important to know the pH of the sample.
Since the creation of the first antibody with high-affinity, molecular imaging techniques has improved. These new technologies make it easier than ever to visualize multiple antigens simultaneously on the same tissue. They use optical filters to selectively visualize different antigens within the same tissue. This allows simultaneous detection for up to 61 different types of antigens on one tissue slice. With the TLK2 marker, image cytometry can be performed for up to 61 different antigens simultaneously.
This antibody recognizes TLK2, a specific arabidopsis nuclear serine/threonine/protein phosphatase. Its calculated molecular mass is 85.3 kD. This protein may be involved with chromatin assembly, as its name suggests. It is active only when phosphorylated and is therefore highly active during the cell division's S phase. It is however less active when DNA damage or blockage to DNA replication causes it to cease working.
TLK2 antibodies have been made that are either monoclonal (or polyclonal) in nature to detect it. These antibodies react to TLK2 in a variety animals. Boster Bio uses rabbit and mouse as model organisms for the development of their TLK2 antibodies. This kinase phosphorylates chromatin-assembling factors, including ASF1A. By preventing proteasome-mediated degradation, phosphorylation of ASF1A enhances chromatin assembly.
PMID: 9427565 by Yamakawa A., et al. cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-alpha and PKU-beta, which have nuclear localization signal.
PMID: 10523312 by Sillje H.H.W., et al. Mammalian homologues of the plant tousled gene code for cell-cycle- regulated kinases with maximal activities linked to ongoing DNA replication.