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Product Info Summary
| SKU: | A06645 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-TLK2 Antibody
SKU/Catalog Number
A06645
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TLK2 Antibody catalog # A06645. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06645)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TLK2. AA range:191-240
Reactive Species
A06645 is reactive to TLK2 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
87.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06645 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using TLK2 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from COLO cells, using TLK2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using TLK2 antibody.
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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-TLK2 Antibody (A06645)
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Customer Q&As
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4 Customer Q&As for Anti-TLK2 Antibody
Question
We are currently using anti-TLK2 antibody A06645 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The anti-TLK2 antibody (A06645) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-19
Question
Is a blocking peptide available for product anti-TLK2 antibody (A06645)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do provide the blocking peptide for product anti-TLK2 antibody (A06645). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-09
Question
I was wanting to use your anti-TLK2 antibody for WB for mouse pubmed:10523312} on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse pubmed:10523312} identification?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
As indicated on the product datasheet, A06645 anti-TLK2 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse pubmed:10523312} in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-02
Question
I am interested in to test anti-TLK2 antibody A06645 on mouse pubmed:10523312} for research purposes, then I may be interested in using anti-TLK2 antibody A06645 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Miller
Verified customer
Asked: 2014-02-17
Answer
The products we sell, including anti-TLK2 antibody A06645, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-02-17
