This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
TLR10 / Toll-like receptor 10 · Western blot design guide
Design a Western Blot for TLR10
Source-linked TLR10 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-TLR10 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
TLR10 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~94.6 kDa | |
| Gel | 8–10% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Cleaved | |
| Caveat | Glycosylation-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked TLR10 Western Blot Protocol Options
The PA1958 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | HELA, JURKAT, CEMJ (catalog PA1958) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | PA1958; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected TLR10 Western Blot Band Size?
TLR10 has a predicted precursor mass of 94.6 kDa; glycosylation, signal-peptide cleavage, and potential homodimerization may affect migration, but no empirical band is supplied.
| Band near 94.6 kDa | Consistent with the predicted precursor mass; confirm identity with controls. |
| Band above 94.6 kDa | Could reflect N-linked glycosylation; its effect on migration is unmeasured. |
| Band below 94.6 kDa | Could reflect cleavage of the 1–19 signal peptide; mature migration is unmeasured. |
| Band near twice the monomer size | Could reflect the annotated potential homodimer if it survives sample preparation. |
💡Expected TLR10 appearanceTLR10 has a predicted precursor mass of 94.6 kDa; signal-peptide cleavage and N-linked glycosylation may affect migration, but no empirical band is supplied, so confirm band identity with ordinary controls.
| Predicted precursor mass | 94.6 kDa is the sequence-based reference, not a measured band. |
| Nine N-linked glycosylation sites, including Asn33, 36, 140, 189, 236, 278, 330, and 416 | Glycosylation may increase or vary apparent size; no shift is quantified. |
| Signal peptide at residues 1–19 | Cleavage makes the mature protein smaller than the precursor; its apparent size is unknown. |
| Potential homodimer | An intact dimer could migrate near twice the monomer size if preserved during preparation. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Membrane TLR10 may be poorly recovered in the lysate. | Check membrane extraction and use a suitable positive control. |
| Band higher than expected | N-linked glycosylation or a preserved potential homodimer may contribute. | Compare deglycosylated and fully denatured samples with controls. |
| Band lower than expected | Cleavage of the 1–19 signal peptide may reduce precursor size. | Check antibody epitope coverage and confirm identity with an independent antibody. |
| Broad smear instead of sharp band | Variable occupancy of annotated N-linked sites is possible. | Compare with a deglycosylated sample; verify band identity. |
| Multiple bands | Different glycosylation or processing states are possible; distinct bands are unproven. | Compare deglycosylated samples and use an independent antibody. |
Experiment evidence
Western blot images showing TLR10 band
PA1958 · TLR10
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TLR10 Western blot
🧪For positive controls for TLR10 in Western blot, you can use a TLR10-positive sample after validation; the supplied HPA data identify none.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: The supplied HPA data identify no positive or negative sample, so both controls need validation.
HPA tissue expression evidence for TLR10
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced TLR10 Western Blot Tips
Deeper troubleshooting and optimisation questions for TLR10, answered from its protein features.
How should TLR10 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could alternative isoforms explain multiple TLR10 bands?
Isoforms · The supplied record lists one isoform and no alternative sequence, so it does not support assigning multiple bands to annotated isoforms. Assess the bands without assuming an isoform identity.
Which TLR10 glycosylation sites matter when interpreting bands?
PTM · The annotated N-linked sites are Asn33, Asn36, Asn140, Asn189, Asn236, Asn278, Asn330, Asn416 and Asn427. These are UniProt full-length sequence coordinates; antibody or paper numbering may differ. The annotations do not show which sites are occupied in a particular sample.
Compare matched treated and untreated samples to test whether N-linked glycans contribute to the band pattern. TLR10 has nine annotated N-linked sites, but their presence alone does not establish that glycosylation causes an observed shift.
Compare matched treated and untreated samples to test whether N-linked glycans contribute to the band pattern. TLR10 has nine annotated N-linked sites, but their presence alone does not establish that glycosylation causes an observed shift.
Does this guide establish induction of TLR10?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for TLR10?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the PA1958 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should TLR10 bands be quantified across samples?
Quantitation · Use the same membrane-protein extraction conditions and the same band-inclusion rule across samples. If several bands appear, report which were quantified; the supplied features do not identify an observed TLR10 band or establish that every band represents TLR10.
Why might TLR10 migrate differently from its predicted 94.6 kDa?
Interpretation · TLR10 has a signal peptide at residues 1–19 and nine annotated N-linked glycosylation sites. These features could affect apparent mass, but the supplied features do not establish a visible shift or its size. No observed band position is available for comparison.
TLR10 is annotated as a single-pass type I membrane protein. Use a sample preparation approach that recovers membrane proteins, and keep extraction conditions consistent when comparing samples.
A TLR10 homodimer is annotated as potential. That makes dimerization a possibility to investigate, but a higher-mass band alone does not establish a dimer. The supplied record gives no observed band positions.
TLR10 is annotated as a single-pass type I membrane protein. Use a sample preparation approach that recovers membrane proteins, and keep extraction conditions consistent when comparing samples.
A TLR10 homodimer is annotated as potential. That makes dimerization a possibility to investigate, but a higher-mass band alone does not establish a dimer. The supplied record gives no observed band positions.
Boster reagents
TLR10 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Toll-like receptor 10 TLR10 Antibody Picoband®
Cat # PA1958
The catalog reports PA1958 as a human-reactive anti-TLR10 antibody with a Western blot image showing HeLa, Jurkat, and CEMJ cell lysates. The supplied record provides no publication evidence or broader sample validation.
Which to pick: PA1958 is the only listed option. Its Western blot image uses HeLa, Jurkat, and CEMJ cell lysates; choose it when those reported contexts and human reactivity fit your experiment.
Source: BosterBio TLR10 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9BXR5.
- Human Protein Atlas. TLR10 tissue expression.
- PMC13115012 — target-verified WB comparison
- PMC11788150 — target-verified WB comparison
- PMC3620725 — target-verified WB comparison