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- Table of Contents
TNNC1 / Troponin C, slow skeletal and cardiac muscles · Western blot design guide
Design a Western Blot for TNNC1
Source-linked TNNC1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-TNNC1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
TNNC1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~18.4 kDa | |
| Gel | 15% (standard starting point) | |
| Positive control | Heart muscle (IHC candidate; verify WB) +1 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked TNNC1 Western Blot Protocol Options
The M03153-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Human fetal heart lysate (catalog M03153-1) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M03153-1; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected TNNC1 Western Blot Band Size?
TNNC1 is predicted at 18.4 kDa; its listed modifications have no demonstrated visible migration effect, and no empirical band size is supplied.
| Single band near 18.4 kDa | consistent with predicted TNNC1 size; confirm identity with controls |
| Band near 18.4 kDa without a separate acetylated band | N-acetylmethionine at residue 1 need not produce a resolvable shift |
| Band near 18.4 kDa without a separate phosphorylated band | phosphoserine at residue 98 need not produce a resolvable shift |
| Additional band away from 18.4 kDa | identity is uncertain without controls; no distinct isoform mass is supplied |
💡Expected TNNC1 appearanceTNNC1 has a predicted mass of 18.4 kDa, but no empirical band size is supplied; assess any band near that size with ordinary identity controls.
| UniProt predicted mass | places the expected full-length band near 18.4 kDa |
| 161-residue sequence | underlies the predicted full-length mass; no cleavage feature is listed |
| N-acetylmethionine at residue 1 | has no established resolvable effect on migration here |
| Phosphoserine at residue 98 | has no established resolvable effect on migration here |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | TNNC1 abundance or assay sensitivity may be insufficient | check sample loading, antibody performance, and a positive heart-muscle control |
| Band higher than expected | the band identity or migration difference is unestablished | compare with a positive control and confirm identity with another antibody |
| Band lower than expected | the band identity or possible protein breakdown is unestablished | use fresh lysate with protease inhibitors and confirm identity |
| Multiple bands | additional bands may reflect nonspecific binding or sample degradation | compare controls and verify the band near 18.4 kDa with another antibody |
| Weak or no signal | low target abundance or inadequate antibody detection | check loading and detection with a positive heart-muscle control |
Experiment evidence
Western blot images showing TNNC1 band
M03153-1 · TNNC1
PA1779 · TNNC1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TNNC1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for TNNC1 in Western blot, you can use heart muscle lysate, which has high HPA expression.
Positive control: Heart muscle (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: HPA reports high expression in muscle and no detection in adipose tissue, making tissue controls feasible.
HPA tissue expression evidence for TNNC1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Heart muscle | cardiomyocytes | High | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced TNNC1 Western Blot Tips
Deeper troubleshooting and optimisation questions for TNNC1, answered from its protein features.
Where should the TNNC1 band appear relative to predicted mass?
Band shift · TNNC1 has a predicted mass of 18.4 kDa. No observed band position is supplied, so treat 18.4 kDa as a reference rather than an established apparent mass. The listed modifications alone do not establish a visible shift.
UniProt lists N-acetylmethionine at position 1 and no signal peptide or propeptide. Do not infer a cleaved precursor from these features. N-terminal acetylation alone does not establish a visible mass shift.
UniProt lists N-acetylmethionine at position 1 and no signal peptide or propeptide. Do not infer a cleaved precursor from these features. N-terminal acetylation alone does not establish a visible mass shift.
Could TNNC1 isoforms explain multiple bands?
Isoforms · The supplied record lists one isoform and no alternative sequence. It therefore provides no isoform-based explanation for multiple bands; verify the identity of any additional bands.
Which TNNC1 phosphorylation site matters when interpreting bands?
PTM · UniProt lists phosphoserine at position 98, using UniProt numbering. Check that antibody or paper numbering matches this convention. The site’s presence does not establish that phosphorylation produces a resolved band shift.
The supplied record lists no glycosylation sites. It offers no glycosylation-based explanation for a higher band; confirm that band’s identity before assigning it to TNNC1.
A total TNNC1 band cannot establish phosphorylation at UniProt position 98. If phosphorylation is the measurement of interest, use a site-specific readout whose residue numbering has been checked against UniProt.
The supplied record lists no glycosylation sites. It offers no glycosylation-based explanation for a higher band; confirm that band’s identity before assigning it to TNNC1.
A total TNNC1 band cannot establish phosphorylation at UniProt position 98. If phosphorylation is the measurement of interest, use a site-specific readout whose residue numbering has been checked against UniProt.
Does this guide establish induction of TNNC1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for TNNC1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M03153-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should TNNC1 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
How should unexpected TNNC1 bands be interpreted?
Interpretation · Use 18.4 kDa as the predicted-mass reference and verify additional bands before assigning them to TNNC1. The supplied features list one isoform, N-acetylmethionine at position 1, and phosphoserine at position 98, but do not establish the apparent position or cause of any extra band.
Boster reagents
TNNC1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Cardiac Troponin C Rabbit Monoclonal Antibody
Cat # M03153-1
Real WB data
Anti-cardiac Troponin C/TNNC1 Antibody Picoband®
Cat # PA1779
Both listed anti-TNNC1 antibodies report human, mouse, and rat reactivity and have Western blot images. The supplied examples use human fetal heart lysate for M03153-1 and rat skeletal muscle lysate for PA1779; these examples do not establish performance in every tissue or species.
Which to pick: Choose M03153-1 if the human fetal heart WB example best matches your sample. Choose PA1779 if its rat skeletal muscle example is more relevant; its caption reports 0.5 µg/mL antibody, 50 µg lysate, and an observed 18 kDa band.
Source: BosterBio TNNC1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P63316.
- Human Protein Atlas. TNNC1 tissue expression.
- PMC7523424 — target-verified WB comparison
- PMC12106437 — target-verified WB comparison
- PMC7578518 — target-verified WB comparison