Product Info Summary
| SKU: | PA1779 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-cardiac Troponin C/TNNC1 Antibody Picoband®
SKU/Catalog Number
PA1779
BA4631 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-cardiac Troponin C/TNNC1 Antibody catalog # PA1779. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-cardiac Troponin C/TNNC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1779)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human cardiac Troponin C, identical to the related rat and mouse sequences .
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1779 is reactive to TNNC1 in Human, Mouse, Rat
Observed Molecular Weight
18 kDa
Calculated molecular weight
18.4 kDa
Background of TNNC1
Troponin C1 (TNNC1) (troponin C type 1 (slow)), also called TROPONIN C, SLOW-TWITCH SKELETAL MUSCLE or TNC, is a protein that in humans is encoded by the TNNC1 gene. Using a "monochromosomal" hybrid panel, the TNNC1 gene is mapped to chromosome 3; subsequent analysis of the Genebridge 4 radiation hybrid panel localized the gene to 3p in a region consistent with the earlier assignment. Circular dichroism measurements revealed changes in the secondary structures of the TNNC1 mutants A8V, C84Y, and D145E.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1779 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Positive Control
WB: Rat Skeletal Muscle Tissue
IHC: Rat Cardiac Muscle Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-cardiac Troponin C antibody, PA1779, Western blotting
All lanes: Anti cardiac Troponin C (PA1779) at 0.5ug/ml
WB: Rat Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 18KD
Observed bind size: 18KD
Click image to see more details
Anti-cardiac Troponin C antibody, PA1779, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Click image to see more details
IHC analysis of TNNC1 using anti-TNNC1 antibody (PA1779).
TNNC1 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNNC1 Antibody (PA1779) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-cardiac Troponin C/TNNC1 Antibody Picoband® (PA1779)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-cardiac Troponin C/TNNC1 Antibody Picoband®
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart muscle using anti-cardiac Troponin C/TNNC1 antibody PA1779. Let me know if you need anything else.
E. Krishna
Verified customer
Asked: 2019-09-18
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
I have attached the WB image, lot number and protocol we used for heart muscle using anti-cardiac Troponin C/TNNC1 antibody PA1779. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-20
Question
Our lab want to know about to test anti-cardiac Troponin C/TNNC1 antibody PA1779 on rat heart muscle for research purposes, then I may be interested in using anti-cardiac Troponin C/TNNC1 antibody PA1779 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The products we sell, including anti-cardiac Troponin C/TNNC1 antibody PA1779, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-05
Question
Will anti-cardiac Troponin C/TNNC1 antibody PA1779 work on goat WB with triceps brachii?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
Our lab technicians have not tested anti-cardiac Troponin C/TNNC1 antibody PA1779 on goat. You can run a BLAST between goat and the immunogen sequence of anti-cardiac Troponin C/TNNC1 antibody PA1779 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat triceps brachii in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
We are currently using anti-cardiac Troponin C/TNNC1 antibody PA1779 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
T. Brown
Verified customer
Asked: 2015-05-20
Answer
The anti-cardiac Troponin C/TNNC1 antibody (PA1779) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-20
Question
I was wanting to use your anti-cardiac Troponin C/TNNC1 antibody for WB for rat heart muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat heart muscle identification?
V. Wu
Verified customer
Asked: 2013-07-18
Answer
It shows on the product datasheet, PA1779 anti-cardiac Troponin C/TNNC1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-18


