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- Table of Contents
TRPC1 / Short transient receptor potential channel 1 · Western blot design guide
Design a Western Blot for TRPC1
Real validated TRPC1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-TRPC1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
TRPC1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~91.2 kDa | |
| Observed band | ~120 kDa | |
| Gel | 10% (catalog M01492) | |
| Positive control | Bone marrow (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated TRPC1 Western Blot Protocols
The M01492 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human PC-3, human HepG2, human Caco-2 (catalog M01492) |
| Gel % | 10% (catalog M01492) |
| Load | 30 ug; reducing conditions (catalog M01492) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog M01492) |
| Membrane | nitrocellulose membrane (catalog M01492) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog M01492) |
| Primary antibody | M01492 · 1:500 (catalog M01492) |
| Primary incubation | overnight at 4°C (catalog M01492) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:1000 (catalog M01492) |
| Secondary incubation | 1.5 hour at RT (catalog M01492) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog M01492) |
| Detection | ECL (catalog M01492) |
Section 2
What Is the Expected TRPC1 Western Blot Band Size?
TRPC1 is predicted at 91.2 kDa but shows an empirical band near 120 kDa; the cause of the difference is not established.
| Band near 120 kDa | Empirical TRPC1 band in reducing whole-cell lysates; confirm identity with controls |
| Band near 91.2 kDa | Near the sequence-predicted mass; identity requires validation |
| Additional discrete bands | Long and Short isoforms are possible contributors, but their migration is unknown |
| Weak band in whole-cell lysate | Membrane localization may limit recovery during extraction |
💡Expected TRPC1 appearanceTRPC1 has a predicted mass of 91.2 kDa, while antibody QC shows a band near 120 kDa in reducing lysates; the cause of this difference is unestablished, so verify band identity with appropriate controls.
| UniProt predicted mass | Places the sequence-based reference at 91.2 kDa, below the observed 120 kDa band |
| Long isoform | May differ in size from Short; its individual mass and migration are unspecified |
| Short isoform | May differ in size from Long; its individual mass and migration are unspecified |
| Alternative splicing | Allows size variation between isoforms, without establishing resolvable bands |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Poor recovery of membrane-localized TRPC1 | Check membrane extraction and a membrane-enriched fraction |
| Band higher than expected | The observed 120 kDa band exceeds the 91.2 kDa prediction for an unknown reason | Compare with the QC band and confirm identity using independent antibody or TRPC1 depletion |
| Band lower than expected | An isoform or fragment is possible, but its size is not established | Check antibody epitope coverage and confirm identity with TRPC1 depletion |
| Multiple bands | Long and Short isoforms are possible contributors; distinct migration is unproven | Compare isoform-specific expression and confirm bands with TRPC1 depletion |
| Weak or no signal | Membrane extraction may be inefficient | Enrich the membrane fraction and check sample loading and transfer |
| Fragments below expected size | Proteolysis during sample preparation is possible | Prepare fresh lysate with protease inhibitors and confirm fragment identity |
Experiment evidence
Western blot images showing TRPC1 band
M01492 · TRPC1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TRPC1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for TRPC1 in Western blot, you can use bone marrow lysate, which HPA scores High.
Positive control: Bone marrow (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA lists no Not-detected tissue, so use TRPC1 knockdown or knockout for a clean negative control.
HPA tissue expression evidence for TRPC1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bone marrow | hematopoietic cells | High | Protein (IHC) | HPA → |
| Breast | glandular cells | High | Protein (IHC) | HPA → |
| Cerebral cortex | neuronal cells | High | Protein (IHC) | HPA → |
| Endometrium | glandular cells | High | Protein (IHC) | HPA → |
| Epididymis | glandular cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Oral mucosa | squamous epithelial cells | Low | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
| Appendix | glandular cells | Medium | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
Section 3
Advanced TRPC1 Western Blot Tips
Deeper troubleshooting and optimisation questions for TRPC1, answered from its protein features.
How should TRPC1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
How might TRPC1 isoforms affect band interpretation?
Isoforms · UniProt lists Long and Short isoforms. The Short isoform lacks residues 110–143 in UniProt numbering, so a lower-mass band is plausible. The features do not establish whether the isoforms resolve as separate bands. Check whether the antibody recognizes a region retained in both isoforms.
Could phosphorylation explain a TRPC1 band shift?
PTM · TRPC1 has a phosphoprotein keyword, but the supplied features list no modified-residue coordinates. Phosphorylation therefore cannot be assigned as the cause of a particular band or the difference between 91.2 and about 120 kDa from this evidence alone.
Does this guide establish induction of TRPC1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for TRPC1 Western blot?
Transfer · TRPC1 is a multi-pass cell-membrane protein with a predicted mass of 91.2 kDa and a supplied apparent band near 120 kDa. Optimize transfer for recovery across that range, then check the membrane and post-transfer gel for retained protein. The supplied features do not identify a preferred transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M01492 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should TRPC1 bands be quantified?
Quantitation · Quantify a band only after confirming its identity, especially when evaluating the supplied band near 120 kDa. Use a consistent band boundary and normalize to total protein in each lane. If Long and Short isoforms resolve separately, report them separately unless the antibody's isoform coverage is known.
Why might TRPC1 appear near 120 kDa rather than 91.2 kDa?
Interpretation · The supplied apparent band is about 120 kDa, while the predicted mass is 91.2 kDa. TRPC1 is a multi-pass membrane protein, but its listed features do not establish the cause of this difference. Confirm band identity with a TRPC1-specific depletion control before assigning the 120 kDa band.
UniProt lists one disulfide bond. Compare reduced and nonreduced samples if the band pattern changes between preparations. This feature alone does not predict the direction or size of a mobility change.
TRPC1 forms heterotetramers with TRPC4 and/or TRPC5; a TRPC1:TRPC4 channel can have 1:3 stoichiometry. That interaction does not establish that a complex survives Western-blot preparation. Test band identity with TRPC1 depletion and compare denaturing conditions before assigning a high band to an oligomer.
UniProt lists one disulfide bond. Compare reduced and nonreduced samples if the band pattern changes between preparations. This feature alone does not predict the direction or size of a mobility change.
TRPC1 forms heterotetramers with TRPC4 and/or TRPC5; a TRPC1:TRPC4 channel can have 1:3 stoichiometry. That interaction does not establish that a complex survives Western-blot preparation. Test band identity with TRPC1 depletion and compare denaturing conditions before assigning a high band to an oligomer.
Boster reagents
TRPC1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-TRPC1 Rabbit Monoclonal Antibody
Cat # M01492
M01492 is a rabbit monoclonal anti-TRPC1 listed for human, mouse, and rat. Its WB image includes human cell, rat testis, and mouse lung and testis lysates. The reported band is approximately 120 kDa, versus an expected 91 kDa, leaving band identity uncertain.
Which to pick: M01492 is the only listed option. Its WB image shows PC-3, HepG2, Caco-2, and U251 cell lysates, plus rat testis and mouse lung and testis lysates. Choose based on your sample context and investigate the reported band size discrepancy.
Source: BosterBio TRPC1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P48995.
- Human Protein Atlas. TRPC1 tissue expression.
- PMC5671818 — target-verified WB comparison
- PMC7255483 — target-verified WB comparison
- PMC4239700 — target-verified WB comparison