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- Table of Contents
TTC36 / Tetratricopeptide repeat protein 36 · Western blot design guide
Design a Western Blot for TTC36
Source-linked TTC36 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-TTC36 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled October 2026 · For research use; verify linked source records and product datasheet before use
TTC36 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~20.9 kDa | |
| Observed band | 21 kDa | |
| Gel | 5–20% (catalog A18139) | |
| Positive control | Duodenum (IHC candidate; verify WB) +3 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked TTC36 Western Blot Protocol Options
The A18139 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | mouse liver (catalog A18139) |
| Gel % | 5–20% (catalog A18139) |
| Load | 30 ug; reducing conditions (catalog A18139) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A18139) |
| Membrane | nitrocellulose membrane (catalog A18139) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A18139) |
| Primary antibody | A18139 · 0.5 μg/mL (catalog A18139) |
| Primary incubation | overnight at 4°C (catalog A18139) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A18139) |
| Secondary incubation | 1.5 hour at RT (catalog A18139) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A18139) |
| Detection | ECL (catalog A18139) |
Section 2
What Is the Expected TTC36 Western Blot Band Size?
TTC36 is predicted at 20.9 kDa and observed at approximately 21 kDa; the cause of the small difference is not established.
| Band at approximately 21 kDa | Matches the empirical TTC36 band in mouse liver lysate |
| Single band near 20.9 kDa | Consistent with the predicted TTC36 mass; band identity still needs confirmation |
| Several bands at different masses | Could reflect isoforms 1 and 2, but their migration is unreported |
| No band near 21 kDa | The empirically observed TTC36 signal is undetected in this sample |
💡Expected TTC36 appearanceTTC36 has a predicted mass of 20.9 kDa and an empirical band at approximately 21 kDa in mouse liver lysate; confirm band identity with appropriate controls.
| Predicted TTC36 mass | Places the expected protein near 20.9 kDa |
| Alternative splicing | Produces isoforms 1 and 2, whose relative masses are unreported |
| Isoform 1 | May migrate differently from isoform 2; its mass is unreported |
| Isoform 2 | May migrate differently from isoform 1; its mass is unreported |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | TTC36 signal is undetected; the reason is not established | Check loading and detection using the documented mouse liver lysate as a positive control |
| Band higher than expected | Identity of a band above 21 kDa is unconfirmed | Compare with the 21 kDa control band and verify specificity by TTC36 knockdown |
| Band lower than expected | A smaller isoform or unrelated signal is possible; isoform masses are unreported | Compare with the 21 kDa control band and verify specificity by TTC36 knockdown |
| Multiple bands | Isoforms 1 and 2 are annotated, but separate bands are not demonstrated | Identify which bands respond to TTC36 knockdown |
| Weak or no signal | TTC36 detection in the sample is unconfirmed | Check protein loading and antibody detection against the documented mouse liver lysate |
Experiment evidence
Western blot images showing TTC36 band
A18139 · TTC36
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TTC36 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for TTC36 in Western blot, you can use duodenum tissue, where HPA reports high expression.
Positive control: Duodenum (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: A positive–negative tissue comparison is feasible because HPA reports high expression in duodenum and no detection in adipose tissue.
HPA tissue expression evidence for TTC36
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Duodenum | paneth cells | High | Protein (IHC) | HPA → |
| Liver | hepatocytes | High | Protein (IHC) | HPA → |
| Kidney | proximal tubules (cell body) | Medium | Protein (IHC) | HPA → |
| Small intestine | paneth cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | endocrine cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced TTC36 Western Blot Tips
Deeper troubleshooting and optimisation questions for TTC36, answered from its protein features.
How should TTC36 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could TTC36 isoform 2 produce a smaller band?
Isoforms · Possibly. UniProt lists two isoforms; isoform 2 lacks residues 1–59 of the canonical sequence. A smaller band is therefore plausible, but the supplied features do not establish its apparent position on a blot.
An antibody recognizing canonical residues 1–59 may miss isoform 2, which lacks that segment. Check the antibody’s mapped epitope against this UniProt coordinate range before comparing isoform bands.
Decide whether the measurement targets the canonical isoform or both isoforms. Because isoform 2 lacks residues 1–59, verify which bands the antibody detects before combining their signals or comparing samples.
An antibody recognizing canonical residues 1–59 may miss isoform 2, which lacks that segment. Check the antibody’s mapped epitope against this UniProt coordinate range before comparing isoform bands.
Decide whether the measurement targets the canonical isoform or both isoforms. Because isoform 2 lacks residues 1–59, verify which bands the antibody detects before combining their signals or comparing samples.
Can annotated modifications explain a shifted TTC36 band?
PTM · The supplied UniProt features list no modified residues or glycosylation sites. They provide no specific modification-based explanation for a shifted band; a shift alone would not establish a modification.
Does this guide establish induction of TTC36?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for TTC36?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A18139 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should TTC36 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Does the 21 kDa TTC36 band match its predicted mass?
Interpretation · Yes. The observed 21 kDa band is close to the 20.9 kDa predicted mass for the canonical 189-residue sequence. This agreement supports, but does not establish, band identity.
Compare them with the approximately 21 kDa canonical band and consider the listed isoform 2 deletion as one possible reason for a smaller band. The supplied features do not establish the identity of any additional band.
Compare them with the approximately 21 kDa canonical band and consider the listed isoform 2 deletion as one possible reason for a smaller band. The supplied features do not establish the identity of any additional band.
Boster reagents
TTC36 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-TTC36 Antibody Picoband®
Cat # A18139
The catalog reports one anti-TTC36 antibody, A18139, with reported human and mouse reactivity. Its Western blot image shows a band at approximately 21 kDa in mouse liver lysate, matching the expected size. The supplied evidence does not show a human Western blot.
Which to pick: A18139 is the only listed option. Its supplied Western blot image uses mouse liver lysate under reducing conditions; consider that tested context when planning your assay. Human reactivity is listed, but no human Western blot example is supplied.
Source: BosterBio TTC36 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry A6NLP5.
- Human Protein Atlas. TTC36 tissue expression.
- PMC12594589 — target-verified WB comparison