This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
UQCRB / Cytochrome b-c1 complex subunit 7 · Western blot design guide
Design a Western Blot for UQCRB
Source-linked UQCRB Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-UQCRB WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
UQCRB Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~13.5 kDa | |
| Gel | 15% (standard starting point) | |
| Positive control | Adrenal gland (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked UQCRB Western Blot Protocol Options
The A07958 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | extracts of various cell lines, (catalog A07958) |
| Gel % | 15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.2 µm PVDF (standard starting point) |
| Blocking | Blocking buffer: 3% nonfat dry milk in TBST (catalog A07958) |
| Primary antibody | A07958 · 1:1000 (catalog A07958) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Goat Anti-Rabbit IgG, 1:10000 (catalog A07958) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL (catalog A07958) |
Section 2
What Is the Expected UQCRB Western Blot Band Size?
UQCRB has a predicted mass of 13.5 kDa; its isoforms could affect migration, but no empirical band or distinct isoform pattern is established.
| Single band near 13.5 kDa | Consistent with the predicted UQCRB mass; confirm identity with controls |
| Two bands at different positions | Could reflect isoforms 1 and 2, but their migration is not established |
| Weak band in whole-cell lysate | Recovery of this mitochondrial inner-membrane protein may be limited |
| Band away from 13.5 kDa | Its identity and reason for altered migration require verification |
💡Expected UQCRB appearanceUniProt predicts UQCRB at 13.5 kDa; no empirical band size or isoform migration is supplied, so verify any detected band with antibody and target-specific controls.
| UniProt predicted mass | Places the reference band near 13.5 kDa |
| Isoform 1 | Has no supplied isoform-specific mass or migration |
| Isoform 2 | Has no supplied isoform-specific mass or migration |
| Alternative splicing of isoforms 1 and 2 | Could alter apparent size, but distinct bands are not established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Recovery of mitochondrial inner-membrane UQCRB may be limited | Check mitochondrial enrichment and lysate preparation |
| Band higher than expected | The supplied features do not establish the shift | Check molecular-weight markers and confirm identity with target-specific controls |
| Band lower than expected | The supplied features do not establish the shift | Confirm identity with a second antibody or target-depleted sample |
| Multiple bands | Isoforms 1 and 2 are listed, but their band positions are unknown | Use isoform-specific or target-depletion controls to identify the bands |
| Weak or no signal | Mitochondrial inner-membrane protein recovery may be low | Assess mitochondrial enrichment and antibody performance with a positive control |
Experiment evidence
Western blot images showing UQCRB band
A07958 · UQCRB
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for UQCRB Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for UQCRB in Western blot, you can use adrenal gland tissue, which shows high HPA expression.
Positive control: Adrenal gland (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: No tissue is listed as not detected, so use siRNA knockdown or a KO line for a negative control.
HPA tissue expression evidence for UQCRB
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | glandular cells | High | Protein (IHC) | HPA → |
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Breast | glandular cells | High | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | High | Protein (IHC) | HPA → |
| Caudate | glial cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Soft tissue | fibroblasts | Low | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
| Ovary | ovarian stroma cells | Medium | Protein (IHC) | HPA → |
| Smooth muscle | smooth muscle cells | Medium | Protein (IHC) | HPA → |
Section 3
Advanced UQCRB Western Blot Tips
Deeper troubleshooting and optimisation questions for UQCRB, answered from its protein features.
Where should the UQCRB band appear?
Band shift · The predicted mass is 13.5 kDa. No observed band position is supplied, so use 13.5 kDa as a starting point rather than an expected apparent mass.
Could UQCRB isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2. Isoform 2 replaces residues 87–111 with a longer alternative sequence, which could change its apparent size. Whether both isoforms are expressed or resolved on a blot is not established here.
Which UQCRB modifications matter when interpreting bands?
PTM · In UniProt coordinates, residue 2 is N-acetylalanine; lysines 12, 19, 78, 83 and 96 have listed modifications. Acetylation and succinylation are alternate annotations at positions 12 and 78. These features alone do not establish a visible shift or explain a mass difference.
Does this guide establish induction of UQCRB?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for UQCRB Western blot?
Transfer · UQCRB has a predicted mass of 13.5 kDa. Choose transfer conditions and a membrane that retain a small protein, then verify transfer near 13.5 kDa. The supplied features do not specify one transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A07958 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should UQCRB bands be quantified?
Quantitation · UQCRB is a peripheral protein on the matrix side of the mitochondrial inner membrane and a component of complex III. Keep mitochondrial sample preparation and loading consistent across lanes. If multiple bands appear, quantify a consistently identified band; the supplied features do not establish which band represents each isoform.
How should unexpected UQCRB bands be interpreted?
Interpretation · Compare them with the 13.5 kDa prediction and consider the two listed isoforms, including isoform 2’s replacement of residues 87–111. The listed modifications do not, by themselves, identify a shifted band. No observed band position is supplied for comparison.
Boster reagents
UQCRB Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-UQCRB Antibody
Cat # A07958
A07958 is listed as reactive with human, mouse, and rat UQCRB. Its Western blot image uses extracts from various cell lines, 25 µg per lane, and a 1:1000 primary dilution. The caption does not identify the cell lines.
Which to pick: A07958 is the only listed option. It has a Western blot image, but the unnamed cell lines do not establish which of the listed species were tested in that image.
Source: BosterBio UQCRB gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P14927.
- Human Protein Atlas. UQCRB tissue expression.
- PMC6122973 — target-verified WB comparison
- PMC6098055 — target-verified WB comparison
- PMC7029028 — target-verified WB comparison