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- Table of Contents
UQCRC1 / Cytochrome b-c1 complex subunit 1, mitochondrial · Western blot design guide
Design a Western Blot for UQCRC1
Real validated UQCRC1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-UQCRC1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
UQCRC1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~52.6 kDa | |
| Observed band | ~45 kDa | |
| Gel | 12% (catalog A06974-1) | |
| Positive control | Adrenal gland (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Band identity controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated UQCRC1 Western Blot Protocols
The A06974-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Hela, human HepG2, human A549 (catalog A06974-1) |
| Gel % | 12% (catalog A06974-1) |
| Load | 30 ug; reducing conditions (catalog A06974-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A06974-1) |
| Membrane | nitrocellulose membrane (catalog A06974-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A06974-1) |
| Primary antibody | A06974-1 · 1:1000 (catalog A06974-1) |
| Primary incubation | overnight at 4°C (catalog A06974-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A06974-1) |
| Secondary incubation | 1.5 hour at RT (catalog A06974-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A06974-1) |
| Detection | ECL (catalog A06974-1) |
Section 2
What Is the Expected UQCRC1 Western Blot Band Size?
UQCRC1 is predicted at 52.6 kDa, but antibody QC shows approximately 45 kDa; the cause of this difference is not established.
| Band near 45 kDa | Empirical UQCRC1 band reported in reducing whole-cell lysates; confirm identity with controls |
| Band near 52.6 kDa | Near the predicted sequence mass; identity requires confirmation |
| Faint band near 45 kDa in whole-cell lysate | UQCRC1 signal may be limited by recovery of its mitochondrial membrane compartment |
| No band in a mitochondrial-depleted fraction | Consistent with UQCRC1 localization at the mitochondrial inner membrane |
💡Expected UQCRC1 appearanceUQCRC1 has a predicted mass of 52.6 kDa, while antibody QC reports a band near 45 kDa in reducing whole-cell lysates; the difference is unexplained, so confirm identity with ordinary controls.
| Predicted sequence mass | 52.6 kDa provides a reference, while observed migration is near 45 kDa |
| N6-acetyllysine at residues 111, 138, 163, and 248 | No visible band-size effect is established |
| N6-succinyllysine at residue 163 | An alternate modification at this site; no visible size effect is established |
| Phosphoserine at residue 212 | No visible band-size effect is established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Limited recovery of mitochondrial inner-membrane material | Check mitochondrial protein recovery and probe a mitochondrial loading control |
| Band lower than expected | The reported 45 kDa band is below the 52.6 kDa predicted mass; the cause is unknown | Compare with the reported QC pattern and confirm identity by knockdown or a second antibody |
| Band higher than expected | Identity or migration of the extra band is uncertain | Compare with the 45 kDa QC band and verify specificity by knockdown |
| Multiple bands | Additional band identities are unestablished for the single listed isoform | Compare controls and test which bands decrease after UQCRC1 knockdown |
| Weak or no signal | Low mitochondrial protein recovery or low target abundance in the sample | Check sample loading and mitochondrial enrichment |
| Fragments below expected size | Possible sample degradation; no fragment size is established | Prepare fresh lysate with protease inhibitors and verify bands with a second antibody |
Experiment evidence
Western blot images showing UQCRC1 band
A06974-1 · UQCRC1
A06974 · UQCRC1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for UQCRC1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for UQCRC1 in Western blot, you can use adrenal gland tissue, which has high HPA expression.
Positive control: Adrenal gland (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: No HPA tissue is listed as not detected, so use siRNA knockdown or a KO line for a negative control.
HPA tissue expression evidence for UQCRC1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | glandular cells | High | Protein (IHC) | HPA → |
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | High | Protein (IHC) | HPA → |
| Caudate | glial cells | High | Protein (IHC) | HPA → |
| Cerebellum | cells in molecular layer | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Low | Protein (IHC) | HPA → |
| Ovary | ovarian stroma cells | Low | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
| Breast | glandular cells | Medium | Protein (IHC) | HPA → |
| Fallopian tube | glandular cells | Medium | Protein (IHC) | HPA → |
Section 3
Advanced UQCRC1 Western Blot Tips
Deeper troubleshooting and optimisation questions for UQCRC1, answered from its protein features.
How should UQCRC1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could UQCRC1 isoforms explain multiple bands?
Isoforms · Only one isoform is supplied, with no alternative sequence. These features provide no isoform-based explanation for multiple bands; verify the identity of each band.
Which UQCRC1 modifications are listed?
PTM · In UniProt sequence numbering, N6-acetyllysine is listed at 111, 138, 163 and 248; N6-succinyllysine is an alternate modification at 163; and phosphoserine is listed at 212. These annotations alone do not establish a visible band shift.
Phosphoserine is listed at UniProt position 212. Its presence does not establish that phosphorylation causes a visible shift. Compare band patterns after phosphatase treatment if testing that possibility; keep UniProt numbering distinct from any antibody or paper numbering.
Phosphoserine is listed at UniProt position 212. Its presence does not establish that phosphorylation causes a visible shift. Compare band patterns after phosphatase treatment if testing that possibility; keep UniProt numbering distinct from any antibody or paper numbering.
Does this guide establish induction of UQCRC1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for UQCRC1 Western blot?
Transfer · Choose transfer conditions suitable for a roughly 45–53 kDa protein and check transfer with a total-protein stain. UQCRC1 is a peripheral protein on the matrix side of the mitochondrial inner membrane; these features do not specify wet or semi-dry transfer.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A06974-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should I quantify UQCRC1 across samples?
Quantitation · Measure the same verified UQCRC1 band at exposures within the linear detection range and normalize to total protein. Because UQCRC1 is mitochondrial, changes in mitochondrial content can affect comparisons between whole-cell samples.
Why might UQCRC1 run near 45 kDa instead of 52.6 kDa?
Interpretation · The observed band is about 45 kDa, while the predicted mass is 52.6 kDa. A transit peptide is listed as a keyword, but no cleavage position is supplied, so these features do not establish why the masses differ. Confirm the band’s identity experimentally.
Compare them with the observed approximately 45 kDa band and the 52.6 kDa predicted mass, then test antibody specificity using UQCRC1 depletion. The supplied record lists one isoform and several modifications, but neither establishes the identity of an additional band.
Compare them with the observed approximately 45 kDa band and the 52.6 kDa predicted mass, then test antibody specificity using UQCRC1 depletion. The supplied record lists one isoform and several modifications, but neither establishes the identity of an additional band.
Boster reagents
UQCRC1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-UQCRC1 Antibody
Cat # A06974-1
Real WB data
Anti-UQCRC1 Antibody
Cat # A06974
Both listed anti-UQCRC1 antibodies have Western blot images. A06974-1 shows human cell, monkey cell, and rat and mouse tissue lysates; its reported band is approximately 45 kDa versus an expected 53 kDa. A06974’s caption reports various cell line extracts without naming them.
Which to pick: For monkey samples, A06974-1 is the only listed option with monkey reactivity and a named monkey sample in its WB caption. For human, mouse, or rat samples, both list reactivity; A06974-1 provides more specific sample details, though its observed band differs from the expected size.
Source: BosterBio UQCRC1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P31930.
- Human Protein Atlas. UQCRC1 tissue expression.
- PMC13059122 — target-verified WB comparison
- PMC12909848 — target-verified WB comparison
- PMC12954188 — target-verified WB comparison