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- Table of Contents
UQCRC2 / Cytochrome b-c1 complex subunit 2, mitochondrial · Western blot design guide
Design a Western Blot for UQCRC2
Real validated UQCRC2 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-UQCRC2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
UQCRC2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~48.4 kDa | |
| Observed band | ~48 kDa | |
| Gel | 10% (catalog A07937-1) | |
| Positive control | Colon (IHC candidate; verify WB) +4 more | |
| Negative control | Oral mucosa (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated UQCRC2 Western Blot Protocols
The A07937-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human HepG2, human K562, rat heart (catalog A07937-1) |
| Gel % | 10% (catalog A07937-1) |
| Load | 30 ug; reducing conditions (catalog A07937-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A07937-1) |
| Membrane | nitrocellulose membrane (catalog A07937-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A07937-1) |
| Primary antibody | A07937-1 · 0.1 μg/mL (catalog A07937-1) |
| Primary incubation | overnight at 4°C (catalog A07937-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A07937-1) |
| Secondary incubation | 1.5 hour at RT (catalog A07937-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A07937-1) |
| Detection | ECL (catalog A07937-1) |
Section 2
What Is the Expected UQCRC2 Western Blot Band Size?
UQCRC2 is predicted at 48.4 kDa and observed at ~48 kDa; the cause of the small difference is not established.
| Band at ~48 kDa | Matches the empirical UQCRC2 band near its 48.4 kDa predicted mass |
| Single sharp band near 48 kDa | Consistent with the one listed isoform |
| Faint band near 48 kDa in whole-cell lysate | May reflect the amount of mitochondrial UQCRC2 in the sample |
| Additional bands away from 48 kDa | Not explained by the one listed isoform; verify their identity |
💡Expected UQCRC2 appearanceUQCRC2 has a predicted mass of 48.4 kDa and an empirical band at ~48 kDa; confirm band identity with ordinary specificity controls.
| Predicted molecular mass | 48.4 kDa places the expected monomer band near the observed ~48 kDa |
| Lysine 66 acetylation | Listed modification; no visible size effect is established |
| Lysine 199 acetylation | Listed modification; no visible size effect is established |
| Lysine 250 acetylation | Listed modification; no visible size effect is established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Mitochondrial UQCRC2 may be scarce in the sampled lysate | Check mitochondrial enrichment and include a positive-control lysate |
| Band higher than expected | No listed feature establishes a higher migrating UQCRC2 band | Check band identity with a second antibody or UQCRC2-depleted sample |
| Band lower than expected | No signal peptide or propeptide cleavage is listed to explain it | Compare with a positive control and verify band identity |
| Multiple bands | Only one isoform is listed | Check specificity with UQCRC2 depletion and compare the ~48 kDa band |
| Weak or no signal | Mitochondrial protein abundance or recovery may be low | Assess mitochondrial recovery and include a positive-control lysate |
Experiment evidence
Western blot images showing UQCRC2 band
A07937-1 · UQCRC2
M07937 · UQCRC2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for UQCRC2 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for UQCRC2 in Western blot, you can use colon tissue, where HPA reports high expression.
Positive control: Colon (IHC candidate; verify WB)
Negative control: Oral mucosa (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: Mitochondrial enrichment may improve detection of this inner-membrane protein.
HPA tissue expression evidence for UQCRC2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Colon | enterocytes | High | Protein (IHC) | HPA → |
| Duodenum | enterocytes | High | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | High | Protein (IHC) | HPA → |
| Skin | melanocytes | High | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Oral mucosa | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Pancreas | exocrine glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | enterocytes | Low | Protein (IHC) | HPA → |
| Epididymis | glandular cells | Low | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | Low | Protein (IHC) | HPA → |
Section 3
Advanced UQCRC2 Western Blot Tips
Deeper troubleshooting and optimisation questions for UQCRC2, answered from its protein features.
How should UQCRC2 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could UQCRC2 isoforms explain multiple bands?
Isoforms · The supplied record lists one isoform and no alternative sequence, so it does not support assigning additional bands to UQCRC2 isoforms.
Could acetylation change UQCRC2 migration?
PTM · UniProt lists N6-acetyllysine at positions 66, 199, and 250, numbered against the supplied UniProt sequence. These sites alone do not establish a visible band shift; compare treated and control samples before attributing a migration difference to acetylation.
The supplied record lists no glycosylation sites. It therefore provides no basis for assigning an unexpected band to a glycosylated UQCRC2 form. Check band identity before interpreting it as UQCRC2.
The supplied record lists no glycosylation sites. It therefore provides no basis for assigning an unexpected band to a glycosylated UQCRC2 form. Check band identity before interpreting it as UQCRC2.
Does this guide establish induction of UQCRC2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for UQCRC2?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A07937-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should UQCRC2 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Does the observed UQCRC2 band match its predicted mass?
Interpretation · Yes. The observed band is approximately 48 kDa, close to the predicted 48.4 kDa. The supplied features do not establish a modification-related shift.
UQCRC2 is a peripheral protein on the matrix side of the mitochondrial inner membrane. Comparing mitochondrial and other fractions can help assess whether a candidate band follows its reported location, though enrichment alone does not establish identity.
Compare equivalent mitochondrial preparations and quantify bands within the assay’s linear signal range. UQCRC2 is a component of the 11-subunit complex III, so a change in its band alone does not establish a change in the entire complex.
UQCRC2 is a peripheral protein on the matrix side of the mitochondrial inner membrane. Comparing mitochondrial and other fractions can help assess whether a candidate band follows its reported location, though enrichment alone does not establish identity.
Compare equivalent mitochondrial preparations and quantify bands within the assay’s linear signal range. UQCRC2 is a component of the 11-subunit complex III, so a change in its band alone does not establish a change in the entire complex.
Boster reagents
UQCRC2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-UQCRC2 Antibody Picoband®
Cat # A07937-1
Real WB data
Anti-UQCRC2 Monoclonal Antibody
Cat # M07937
The catalog reports two anti-UQCRC2 antibodies for Western blot: A07937-1 (polyclonal) and M07937 (monoclonal). Both list human, mouse and rat reactivity and show an approximately 48 kDa band in the captioned lysates. Evidence is limited to those reported blots.
Which to pick: Both have WB images. Choose A07937-1 for the reported heart or colon samples; choose M07937 for the reported brain or 293T samples. Both include kidney and human cell lysates. Match the published sample and antibody dilution to your experiment.
Source: BosterBio UQCRC2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P22695.
- Human Protein Atlas. UQCRC2 tissue expression.
- PMC8632272 — target-verified WB comparison
- PMC12100729 — target-verified WB comparison
- PMC11662480 — target-verified WB comparison