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- Table of Contents
WDR13 / WD repeat-containing protein 13 · Western blot design guide
Design a Western Blot for WDR13
Source-linked WDR13 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-WDR13 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled October 2026 · For research use; verify linked source records and product datasheet before use
WDR13 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~53.7 kDa | |
| Observed band | 56 kDa | |
| Gel | 5–20% (catalog A13007-1) | |
| Positive control | Cerebellum (IHC candidate; verify WB) +4 more | |
| Negative control | Soft tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Source-Linked WDR13 Western Blot Protocol Options
The A13007-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Hela, human A549 (catalog A13007-1) |
| Gel % | 5–20% (catalog A13007-1) |
| Load | 30 ug; reducing conditions (catalog A13007-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A13007-1) |
| Membrane | nitrocellulose membrane (catalog A13007-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A13007-1) |
| Primary antibody | A13007-1 · 0.5 μg/mL (catalog A13007-1) |
| Primary incubation | overnight at 4°C (catalog A13007-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A13007-1) |
| Secondary incubation | 1.5 hour at RT (catalog A13007-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A13007-1) |
| Detection | ECL (catalog A13007-1) |
Section 2
What Is the Expected WDR13 Western Blot Band Size?
WDR13 is predicted at 53.7 kDa and observed near 56 kDa; the cause of the difference is not established.
| Band near 56 kDa | Empirical WDR13 band in reducing whole-cell lysates; confirm identity with appropriate controls |
| Band near 53.7 kDa | Consistent with the predicted WDR13 mass, pending identity confirmation |
| Two bands near the expected region | Could reflect isoforms 1 and 2, though distinct migration is not established |
| Weak whole-cell band | WDR13 is nuclear; compare with a nuclear-enriched fraction |
💡Expected WDR13 appearanceWDR13 has a predicted mass of 53.7 kDa and an empirical band near 56 kDa in reducing whole-cell lysates; confirm band identity with controls because the cause of the difference is not established.
| Predicted WDR13 mass | 53.7 kDa is the sequence-based reference; the empirical band is near 56 kDa |
| Isoform 1 | Its individual mass and migration are not supplied |
| Isoform 2 | Its individual mass and migration are not supplied |
| Alternative splicing | Isoforms 1 and 2 could differ in size, but distinct bands are not established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | WDR13 is nuclear, so its signal may be weak in the tested lysate | Check a nuclear-enriched fraction and a positive-control lysate |
| Band higher than expected | The observed 56 kDa band exceeds the 53.7 kDa prediction; the cause is unestablished | Compare with the reported 56 kDa band and verify identity by WDR13 depletion |
| Band lower than expected | An isoform difference is possible, but no isoform mass is supplied | Check antibody epitope coverage and verify the band by WDR13 depletion |
| Multiple bands | Isoforms 1 and 2 are annotated, but their separation on a blot is unestablished | Use WDR13 depletion and isoform-specific controls where available |
| Weak or no signal | Nuclear WDR13 may be underrepresented in the tested sample | Check nuclear enrichment, loading, and a positive-control lysate |
Experiment evidence
Western blot images showing WDR13 band
A13007-1 · WDR13
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for WDR13 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for WDR13 in Western blot, you can use cerebellum tissue, which HPA scores as high expression.
Positive control: Cerebellum (IHC candidate; verify WB)
Negative control: Soft tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: WDR13 is nuclear, so nuclear-enriched lysate may improve detection.
HPA tissue expression evidence for WDR13
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | Bergmann glia - nucleus | High | Protein (IHC) | HPA → |
| Lung | alveolar cells type I | High | Protein (IHC) | HPA → |
| Testis | Leydig cells | High | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Soft tissue | fibroblasts | Not detected | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Low | Protein (IHC) | HPA → |
| Appendix | glandular cells | Low | Protein (IHC) | HPA → |
| Duodenum | glandular cells | Low | Protein (IHC) | HPA → |
| Endometrium | cells in endometrial stroma | Low | Protein (IHC) | HPA → |
Section 3
Advanced WDR13 Western Blot Tips
Deeper troubleshooting and optimisation questions for WDR13, answered from its protein features.
How should WDR13 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
How could WDR13 isoforms affect the band pattern?
Isoforms · UniProt lists isoforms 1 and 2. Isoform 2 lacks residues 1–92 of the canonical sequence, so it may produce a smaller band. An antibody targeting that missing region would not detect isoform 2. Check the antibody epitope when interpreting bands.
Which phosphorylation sites matter when interpreting WDR13 bands?
PTM · UniProt annotates phosphoserines at canonical residues 70, 74 and 79. All three lie within residues 1–92, which are missing from isoform 2. A phosphorylation-sensitive signal at these sites therefore concerns the canonical sequence. Their presence does not establish a visible band shift.
At canonical residue 114, UniProt lists alternative annotations: asymmetric dimethylarginine and omega-N-methylarginine; the latter annotation also mentions alternate N-acetylmethionine. Keep UniProt's canonical numbering when comparing antibody or paper annotations. These records alone cannot identify which modification is present in a particular band.
At canonical residue 114, UniProt lists alternative annotations: asymmetric dimethylarginine and omega-N-methylarginine; the latter annotation also mentions alternate N-acetylmethionine. Keep UniProt's canonical numbering when comparing antibody or paper annotations. These records alone cannot identify which modification is present in a particular band.
Does this guide establish induction of WDR13?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for WDR13?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A13007-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should WDR13 bands be quantified across samples?
Quantitation · Quantify a consistently identified band or isoform across samples. WDR13 is annotated in the nucleus, and its two isoforms and modification sites can complicate comparisons if different bands are combined. Record which band is measured and use the same sample preparation across comparisons.
Why might WDR13 appear at 56 kDa instead of 53.7 kDa?
Interpretation · The observed band is 56 kDa, while the predicted mass is 53.7 kDa. WDR13 has annotated modifications and two isoforms, but those features alone do not establish the cause of this difference. Check whether the band is WDR13 before assigning a cause.
First compare them with the 56 kDa observed band and the 53.7 kDa predicted mass. Isoform 2 lacks canonical residues 1–92, providing a reason to investigate a smaller band. Annotated modifications are additional possibilities to examine, but the feature list alone cannot assign an unexpected band to an isoform or modification.
First compare them with the 56 kDa observed band and the 53.7 kDa predicted mass. Isoform 2 lacks canonical residues 1–92, providing a reason to investigate a smaller band. Annotated modifications are additional possibilities to examine, but the feature list alone cannot assign an unexpected band to an isoform or modification.
Boster reagents
WDR13 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-WDR13 Antibody Picoband®
Cat # A13007-1
The catalog reports one anti-WDR13 antibody, A13007-1, with stated human reactivity. Its WB image shows an approximately 56 kDa band in HeLa and A549 whole-cell lysates, compared with an expected 54 kDa. The supplied evidence is limited to this example.
Which to pick: A13007-1 is the only listed option. Its WB image uses human HeLa and A549 whole-cell lysates under reducing conditions, with antibody at 0.5 μg/mL. Use that example when planning a human-sample blot.
Source: BosterBio WDR13 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q9H1Z4.
- Human Protein Atlas. WDR13 tissue expression.
- PMC3371019 — target-verified WB comparison
- PMC5320654 — target-verified WB comparison
- PMC4549788 — target-verified WB comparison