This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
WNT11 / Protein Wnt-11 · Western blot design guide
Design a Western Blot for WNT11
Real validated WNT11 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-WNT11 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
WNT11 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~39.2 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Cleaved | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated WNT11 Western Blot Protocols
The A03705 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Target-positive lysate and matched negative control (standard starting point) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A03705; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected WNT11 Western Blot Band Size?
WNT11 has a predicted full-length mass of 39.2 kDa; signal-peptide cleavage and N-linked glycosylation could affect migration, but no empirical band size is supplied.
| Band near 39.2 kDa | Compatible with the predicted full-length precursor; confirm its identity. |
| Band below 39.2 kDa | Could reflect removal of the 1–24 signal peptide; its migration is not established. |
| Band above 39.2 kDa | Could reflect N-linked glycosylation; site annotations alone do not establish a shift. |
| Little or no band in whole-cell lysate | WNT11 is secreted into the extracellular space and matrix. |
💡Expected WNT11 appearanceThe predicted full-length mass is 39.2 kDa; signal-peptide removal and N-linked glycosylation may affect migration, but no empirical band size is supplied, so confirm identity with appropriate controls.
| Predicted full-length mass | 39.2 kDa is the sequence-based precursor reference, not a measured band. |
| Signal peptide at residues 1–24 | Its removal makes the mature chain smaller than the precursor; apparent migration is unknown. |
| N-linked glycosylation at Asn40 | May affect apparent size if occupied; no shift is established. |
| N-linked glycosylation at Asn90 | May affect apparent size if occupied; no shift is established. |
| N-linked glycosylation at Asn160 | May affect apparent size if occupied; no shift is established. |
| N-linked glycosylation at Asn300 | May affect apparent size if occupied; no shift is established. |
| N-linked glycosylation at Asn304 | May affect apparent size if occupied; no shift is established. |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | WNT11 is secreted and associated with extracellular matrix. | Check conditioned medium or extracellular matrix fractions alongside lysate. |
| Band higher than expected | N-linked glycosylation may affect migration. | Compare untreated and deglycosylated samples with a positive control. |
| Band lower than expected | Removal of the 1–24 signal peptide may yield a smaller mature chain. | Check antibody epitope coverage and compare cellular and secreted fractions. |
| Broad smear instead of sharp band | Variable N-linked glycosylation is possible but unproven. | Compare untreated and deglycosylated samples and confirm antibody specificity. |
| Multiple bands | Precursor, mature, or differently glycosylated forms are possible; distinct bands are not established. | Compare cellular and secreted fractions with deglycosylation and a positive control. |
| Weak or no signal | Secretion may leave little WNT11 in the sampled fraction. | Check conditioned medium or extracellular matrix fractions with a positive control. |
Experiment evidence
Western blot images showing WNT11 band
A03705 · WNT11
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for WNT11 Western blot
🧪For positive controls for WNT11 in Western blot, you can use conditioned medium from a positive tissue or cell once one is validated; the supplied HPA evidence identifies none.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: WNT11 is secreted, so conditioned medium may give a clearer signal than whole-cell lysate.
HPA tissue expression evidence for WNT11
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced WNT11 Western Blot Tips
Deeper troubleshooting and optimisation questions for WNT11, answered from its protein features.
How should WNT11 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Can WNT11 isoforms explain multiple bands?
Isoforms · The supplied record lists one isoform and no alternative sequence. It therefore provides no annotated isoform explanation for multiple bands. Verify band identity before assigning an unexpected band to WNT11.
Which WNT11 glycosylation sites should I consider?
PTM · UniProt lists N-linked sites at Asn40, Asn90, Asn160, Asn300, and Asn304. These are UniProt precursor coordinates; check the numbering convention before comparing them with antibody or paper coordinates. Compare matched samples with and without N-glycan removal to assess whether glycosylation affects a band.
Does this guide establish induction of WNT11?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for WNT11 Western blot?
Transfer · These features do not specify a transfer method. Use the predicted 39.2 kDa precursor mass as a starting point, then check membrane recovery and adjust transfer conditions empirically for your samples.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A03705 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should WNT11 bands be quantified?
Quantitation · Compare the same sample fraction and preparation across conditions because WNT11 is secreted and matrix-associated. Define which verified band or bands are measured, and apply that choice consistently; the supplied features do not establish an apparent band position.
Why might WNT11 migrate differently from its predicted 39.2 kDa?
Interpretation · The 39.2 kDa prediction does not account for every processed form. WNT11 has a signal peptide at residues 1–24 and five annotated N-linked glycosylation sites. These features can affect apparent mass, but no observed band is supplied, so they cannot explain a specific difference.
WNT11 is annotated as secreted and located in the extracellular space and extracellular matrix. Consider the medium and matrix-associated fraction when choosing samples, and compare the same fraction across conditions.
Check band identity before assigning a cause. The signal peptide, five N-linked glycosylation sites, and 11 disulfide bonds provide processing and sample-preparation factors to investigate. The record supplies one isoform and no observed band, so it cannot identify an unexpected band from its position alone.
WNT11 is annotated as secreted and located in the extracellular space and extracellular matrix. Consider the medium and matrix-associated fraction when choosing samples, and compare the same fraction across conditions.
Check band identity before assigning a cause. The signal peptide, five N-linked glycosylation sites, and 11 disulfide bonds provide processing and sample-preparation factors to investigate. The record supplies one isoform and no observed band, so it cannot identify an unexpected band from its position alone.
Boster reagents
WNT11 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Protein Wnt-11 WNT11 Antibody
Cat # A03705
A03705 is an anti-WNT11 antibody listed for human and mouse reactivity. Its Western blot image shows K562 cell lysates using a 1:1000 primary antibody dilution overnight at 4°C; the supplied evidence covers this sample and condition only.
Which to pick: A03705 is the only listed WNT11 antibody. It has a Western blot image from K562 lysates; check whether its listed human and mouse reactivity and reported conditions fit your experiment.
Source: BosterBio WNT11 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O96014.
- Human Protein Atlas. WNT11 tissue expression.
- PMC5436520 — target-verified WB comparison
- PMC4756373 — target-verified WB comparison
- PMC2824771 — target-verified WB comparison