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- Table of Contents
ZNF217 / Zinc finger protein 217 · Western blot design guide
Design a Western Blot for ZNF217
Source-linked ZNF217 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-ZNF217 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
ZNF217 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~115.3 kDa | |
| Gel | 8–10% (standard starting point) | |
| Positive control | Cervix (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Ubl conjugation | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked ZNF217 Western Blot Protocol Options
The M03921-2 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Caco2 cell lysate (catalog M03921-2) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Wet/tank transfer; optimize duration (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M03921-2; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected ZNF217 Western Blot Band Size?
ZNF217 is predicted at 115.3 kDa; annotated modifications could affect migration, but no visible shift or empirical band size is established.
| Band near 115.3 kDa | consistent with the predicted full-length ZNF217 mass; confirm its identity with antibody controls |
| Faint band in whole-cell lysate | nuclear ZNF217 may be easier to detect after nuclear enrichment |
| Band above 115.3 kDa | could reflect a modified form, but its identity and migration are unestablished |
| Doublet near 115.3 kDa | could reflect different modification states; confirm both bands as ZNF217 |
💡Expected ZNF217 appearanceFull-length ZNF217 has a predicted mass of 115.3 kDa, but no empirical band size is supplied; verify any candidate band with ordinary band-identity controls.
| Predicted full-length mass | provides a 115.3 kDa reference, not a measured migration position |
| Phosphoserine sites | are annotated, but no apparent size change is established |
| Phosphothreonine sites | are annotated, but no apparent size change is established |
| Ubl conjugation annotation | may affect migration, but no band position is supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | nuclear localization may limit detection in the sampled fraction | check a nuclear fraction and confirm sample loading |
| Band higher than expected | a modified ZNF217 form is possible but unconfirmed | confirm identity with an independent antibody or ZNF217 depletion |
| Band lower than expected | the band may be a fragment or nonspecific signal | confirm identity with ZNF217 depletion and a second antibody |
| Multiple bands | modification states or nonspecific binding are possible | test which bands track with ZNF217 depletion |
| Weak or no signal | insufficient nuclear protein or low ZNF217 abundance | enrich the nuclear fraction and check loading and antibody performance |
| Fragments below expected size | sample degradation is possible | check sample handling and confirm fragment identity with a second antibody |
Experiment evidence
Western blot images showing ZNF217 band
M03921-2 · ZNF217
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ZNF217 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for ZNF217 in Western blot, you can use cervix tissue, which HPA scores High.
Positive control: Cervix (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA identifies both high-expression and not-detected tissues, making tissue controls feasible for this nuclear protein.
HPA tissue expression evidence for ZNF217
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cervix | glandular cells | High | Protein (IHC) | HPA → |
| Colon | peripheral nerve/ganglion | High | Protein (IHC) | HPA → |
| Duodenum | glandular cells | High | Protein (IHC) | HPA → |
| Fallopian tube | ciliated cells (cell body) | High | Protein (IHC) | HPA → |
| Kidney | cells in tubules | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Spleen | cells in red pulp | Not detected | Protein (IHC) | HPA → |
| Cerebral cortex | endothelial cells | Low | Protein (IHC) | HPA → |
| Hippocampus | glial cells | Low | Protein (IHC) | HPA → |
Section 3
Advanced ZNF217 Western Blot Tips
Deeper troubleshooting and optimisation questions for ZNF217, answered from its protein features.
How should ZNF217 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could documented isoforms explain multiple ZNF217 bands?
Isoforms · UniProt lists one isoform and no alternative sequence. This record therefore does not support assigning multiple bands to documented isoforms; verify additional bands independently.
Which ZNF217 phosphorylation sites matter when interpreting bands?
PTM · UniProt lists phosphoserine at 106, 321, 407, 593, 662 and 795, and phosphothreonine at 322 and 648. These are UniProt sequence coordinates; antibody or paper numbering may differ. Their presence does not establish a detectable mobility shift.
Does this guide establish induction of ZNF217?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for ZNF217 Western blot?
Transfer · Plan transfer around ZNF217’s predicted 115.3 kDa mass. Check transfer efficiency in that size region with a protein stain or marker, and adjust transfer conditions if protein remains in the gel. UniProt does not specify a transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M03921-2 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How can ZNF217 bands be quantified consistently?
Quantitation · Measure the same verified ZNF217 band across samples and normalize to a suitable loading measure for the preparation used. Its listed nuclear location makes consistent sample preparation especially relevant; the UniProt features do not establish which band an antibody detects.
Where should ZNF217 appear relative to its predicted mass?
Interpretation · ZNF217 has a predicted mass of 115.3 kDa. No observed band position is supplied, so use that value as a starting point and confirm candidate bands experimentally. Listed modifications alone do not establish a visible shift.
ZNF217 is listed in the nucleus. If using nuclear extracts, keep the extraction and loading approach consistent across samples and verify that the preparation contains nuclear protein.
Start with the predicted 115.3 kDa mass and the single listed isoform. ZNF217 has eight listed phosphorylation sites, but they do not by themselves identify another band or prove a shift. Verify band identity before attributing additional signals to ZNF217.
ZNF217 is listed in the nucleus. If using nuclear extracts, keep the extraction and loading approach consistent across samples and verify that the preparation contains nuclear protein.
Start with the predicted 115.3 kDa mass and the single listed isoform. ZNF217 has eight listed phosphorylation sites, but they do not by themselves identify another band or prove a shift. Verify band identity before attributing additional signals to ZNF217.
Boster reagents
ZNF217 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-ZNF217 Rabbit Monoclonal Antibody
Cat # M03921-2
The listed anti-ZNF217 rabbit monoclonal antibody, M03921-2, has reported human reactivity and a Western blot image using Caco2 cell lysate. No publication evidence or validation in other samples is supplied.
Which to pick: M03921-2 is the only listed option and the only one with a Western blot image. Its Caco2 lysate example is the relevant evidence when choosing it for a human ZNF217 blot.
Source: BosterBio ZNF217 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O75362.
- Human Protein Atlas. ZNF217 tissue expression.
- PMC5468349 — target-verified WB comparison
- PMC13596211 — target-verified WB comparison
- PMC9069901 — target-verified WB comparison