DNA annealing helicase and endonuclease ZRANB3 (ZRANB3)

DNA annealing helicase and endonuclease needed to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and restricting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an available 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634).

In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity to be able to prevent disintegration of the replication fork and the formation of double-strand fractures (PubMed:22705370, PubMed:22704558). .