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- Table of Contents
Boster Bio Reporter-Labeled Cell Lines
Reporter Cell Lines with Renilla Luciferase
Ready-to-use Renilla luciferase reporter cell lines for quantitative pathway activity and small-molecule screening.
- Ready-to-use validated cell lines
- Get real-time data
- Custom cell lines available
Consult with a reporter cell line expert. We offer a broad panel of validated pathway reporters across commonly used cell backgrounds, with custom reporter cell line generation available.
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Quantitatively measure signaling pathway activity with validated Renilla reporter cell lines.
Get real-time data with Boster Bio's ready-to-use validated reporter cell lines with Renilla luciferase.
Browse reporter cell lines with renilla luciferase
Product
Figures
Species
Applications
Price
1 images
Functional Assay
$5040
Cat # RC1045
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1045
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1 images
Functional Assay
$2072
Cat # RC1044
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1044
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1 images
Functional Assay
$2072
Cat # RC1043
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1043
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1 images
Functional Assay
$2072
Cat # RC1042
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1042
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1 images
Functional Assay
$2072
Cat # RC1041
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1041
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1 images
Functional Assay
$5040
Cat # RC1040
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1040
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1 images
Functional Assay
$7280
Cat # RC1039
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1039
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1 images
Functional Assay
$2072
Cat # RC1038
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1038
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1 images
Functional Assay
$2072
Cat # RC1037
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1037
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1 images
Functional Assay
$2072
Cat # RC1036
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Cat # RC1036
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
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What is a reporter cell line with Renilla luciferase?
Reporter cell lines are common stable cell lines that have been labelled with a reporter gene, such as Renilla luciferase, one of several widely used types of reporter genes. The Renilla luciferase reporter cell line is a stably transfected cell line which expresses the Renilla luciferase reporter gene under the transcriptional control of a selected promoter. The reporter provides a quantitative readout of promoter or response-element activity via bioluminescence. Renilla luciferase reporter cell lines measure transcriptional activity with bioluminescence, in contrast to chromogenic systems such as the reporter gene lacZ.
Reporter cell lines are stably transfected common cell lines that allow for highly sensitive quantification of engrafted target cells. Researchers utilize reporter gene assays to study signaling pathways, gene regulation, and structure of regulatory elements. These reporter genes monitor receptor-mediated cellular processes at the transcriptional level, illustrating core reporter gene function in living cells.
Benefits of using a reporter cell line with Renilla luciferase
- Exceptional quantitation for mammalian cells
- Tight coupling of protein synthesis with enzyme activity
- Rapid and reproducible results
- Easy to perform assay
- No radioactive material handling
- Linear range of activity >5 orders of magnitude
What is Renilla Luciferase
Renilla luciferase is an enzyme from the shallow-water soft coral Renilla reniformis, commonly known as the sea pansy. Renilla's molecular biology and chemistry is well-studied. The monomeric, 36 kDa protein catalyzes a chemical reaction between Renilla luciferase and its substrate coelenterazine responsible for its bioluminescence. The resulting photon emission is a result of the oxidation of coelenterazine to produce light. Renilla luciferase is a powerful monitoring system for biological processes, as no additional post-translational processing is required for enzymatic activity, making it a classic reporter gene example in many assays, alongside other formats such as the reporter gene GFP.
How Does A Renilla Luciferase Reporter Gene Assay Work?
A Renilla luciferase reporter gene assay, utilizes the luciferase enzyme and the substrate coelenterazine to study gene regulation at the transcriptional level.
The Renilla luciferase reporter gene assay monitors your promoter of choice induction activity by screening of the promoter's signaling pathway for activators and inhibitors. The assay allows exploration of a protein's ability to activate or inhibit the expression of a target gene. Using Renilla luciferase as a reporter protein results in a chemical reaction between the enzyme and a coelenterazine substrate, resulting in the emission of photons - bioluminescence. This bioluminescence is quantified as a direct measure of its enzymatic activity.
RENILLA LUCIFERASE REPORTER
CELL LINES Applications
Gene expression and regulation analysis
Signal pathway mapping
Promoter and regulatory element structure analysis
Protein folding and protein-protein interactions, or environmental sensing with an AHR reporter cell line
SNP (single nucleotide polymorphism) analysis
Antiviral research and therapy, or immune checkpoint studies using a PD-1 reporter cell line
Cytotoxicity assay or cytokine signaling studies with a TSLP reporter cell line
Drug discovery
General protocol for Renilla
luciferase reporter cell assay
This protocol may vary per application and experimental objective, but should be similar for either a single reporter or dual reporter assay (dual reporter assays typically use Renilla luciferase as an internal control to normalize experimental variations arising from sample handling and transfection efficiency)
Pick Your Signaling Pathways
| ISRE | STAT4 | TLR/NF-KB | MRE |
| SBE | CRE | SRF-RE | SRE |
| TLR9/NF-KB | TLR8/NF-KB | TLR7/NF-KB | TLR3/NF-KB |
| TLR2/NF-KB | NF-KB | TLR3/ISRE | TLR3/INFB |
| TCF/LEF | STAT3 | NFAT | GATA3 |
| TNF-β | TNF-α | AP-1 |
| ISRE | INOS | STAT1 | NFAT |
| TNF-β | TNF-α | MIP-2 | IL-8 |
| NF-KB |
| ATF6 | P53 | TLR-4/IL-8 | HRE |
| STAT1 |
| MDA/NF-KB | RIG-I/NF-KB |
| STAT4 | STAT5 |
| FOXP3 | NF-KB |
| Nrf2 |
| IL-6 |
Which Cell Lines Have Reporter-Labeled Pathways?
HeLa Cell Lines
Be Aware (& practice good aseptic technique)! Although possible with other cell types, HeLa cells are so notoriously prolific that countless studies over the years have been challenged after it was found that HeLa cells could float on dust particles in the air and contaminate other cell cultures. Good authentication and validation practices will additionally help detect any cross-contamination with other cell lines.
HeLa Cell Lines
HeLa cell lines are the first immortalized and most widely used human cell line. Obtained from a cervical tumor in 1951 during Henrietta Lacks' treatment of cancer at Johns Hopkins, the highly prolific HeLa cell line continues to make a wide-range of diverse contributions towards many different fields of research and medical discovery. For example, HeLa cell lines contributed significantly towards the development of the COVID-19, polio, tetanus, and many other vaccines. HeLa cells are also used for research of cancer and underlying disease mechanisms, AIDS, viral prevalence to cervical cancers (like HPV), the effects of radiation exposure, to test human sensitivity to certain drugs and chemicals, and many more significant discoveries.
The distinct difference between HeLa cells and normal human cells that directly contribute to HeLa's prolific success:
- HeLa cell are cancer cells. And not only that, HeLa cells grow fast. Faster than other cancer cells - by a lot - making them ideal for both research and upstream large scale projects.
Doubling time ≈ 34 hours
HEK293 & HEK293T Cell Lines
HEK293 & HEK293T Cell Lines
Human embryotic kidney (HEK293) cell lines are an extensively used immortalized cell line grown in tissue culture. The original HEK cell line was derived by transformation with sheared Adenovirus 5 DNA. After repeated attempts to cultivate, a rapidly growing, single transformed clone was isolated and dubbed as HEK293. Transfection with adenovirus genes results in a highly prolific model system.
HEK293T cells are a daughter cell line derived from the original HEK293 cells and transfected with a SV40 plasmid vector. The SV40 transfection allows for an even higher production of recombinant proteins.
Doubling time ≈ 33 hours
BaF3 Cell Lines
BaF3 Cell Lines
The Ba/F3 cell line derives from the murine C3H species as a hematopoietic, immortalized pro-B cell line, dependent on IL3 for growth. Ba/F3 cells provide a model system as powerful indicators of kinase activity, such as potency, mutations and downstream signaling, or inhibition of kinase activity.
Protein kinases are popular targets for drug discovery as they have the ability to behave as dominant oncogenes across a wide variety of cancers.
Doubling time ≈ 8-10 hours
FAQs
Q1: What is a reporter cell line?
The Renilla luciferase reporter cell line is a stably transfected cell line which
expresses the Renilla luciferase reporter gene under the transcriptional control of a promoter. The
reporter allows for the monitoring and visualization of specific protein and transcription factor
expression. If a specific signaling pathway is activated, the luciferase is transcribed and the
reporter is expressed as luminescence (light). Renilla luciferase reporter cell lines measure
transcriptional activity in real-time with bioluminescence.
Q2: How are your reporter cell lines validated?
Boster Bio’s proprietary reporter cell lines are extensively validated for functional assay of
small molecules. Our innovative technology delivers increased sensitivity with a prolonged signal
intensity. Each cell line includes documentation of activity validation.
Q3: How should cell lines be handled upon arrival?
It is recommended to thaw cells immediately upon receipt. Cells are shipped on dry ice (not as cold as liquid nitrogen) therefore thawing has begun in shipping. Immediately storing at -80 °C or on liquid nitrogen will cause cell damage thus decreasing cell viability. The thaw cycle is a crucial beginning step for successful propagation. Do not refreeze reporter cells once thawed.
A typical reporter cell line will require you to thaw frozen cells immediately upon receipt from frozen or liquid nitrogen in water bath at 37 °C.
Be sure to check the culture conditions included with your cell lines, or under the product listing on our website.
Q4: Can Boster Bio’s reporter cell lines be used for quantitative measurements?
Yes, you’ll just need to make a standard curve using a positive control. Here’s some
help preparing standards.
Q5: Can Boster Bio’s reporter cell lines be used in other assays, such as Western Blotting or ELISA?
Yes, Boster Bio’s reporter cell lines may be used for other assays.
Please be aware that they are not sold for this purpose.
Q6: How is the luciferase activity measured?
Cell lysates are measured in either multi-well plates or tubes with a luminometer. Luminometers
measure the photon emission produced from the luciferase reaction. Emissions are reported in
relative light units (RLU). The addition of an injector enables measurements immediately, minimizing
diminishing signal and experimental variability, such as pipetting errors.
Q7: How do I determine the amount of background activity?
The background levels of reagent without Renilla luciferase is extremely low, resulting in total
luminescence being directly proportional to luciferase activity. That being said, it is almost
always necessary to take a background reading. This ensures there is no signal from your instrument,
sample tubes/plates, or any autoluminescence directly from the Renilla luciferase. Coelenterazine
may nonenzymatically oxidize in solution.
Ready to accelerate your research?
Explore our validated reporter cell lines or request a custom solution tailored to your pathway of interest.