NF-kB Luciferase Reporter-Jurkat Cell Line

NF-kB reporter cell line

Product Info Summary

SKU: RC1042
Size: 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Application: Functional Assay

Product Name

NF-kB Luciferase Reporter-Jurkat Cell Line

See all NF-kB products

SKU/Catalog Number



1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.


The NF-kB Luciferase Reporter cell line is a stably transfected Jurkat T cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. NF-kB is a key transcription factor that is involved in immune and inflammatory responses, developmental processes, cellular growth and apoptosis. The NF-kB induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1. 


Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

Gene Name


Storage & Handling

Immediately upon receipt, store in liquid nitrogen.

Cite This Product

NF-kB Luciferase Reporter-Jurkat Cell Line (Boster Biological Technology, Pleasanton CA, USA, Catalog # RC1042)

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.


Monitor the NF-kB signaling pathway activity.Screen for activators or inhibitors of the NF-kB signaling pathway.

Culture conditions:

Cells should be grown at 37°C with 5% CO2 using RPMI medium supplemented with 10% FBS, 1 mM sodium pyruvate, 10 mM HEPES and 1% Pen/Strep plus 3 µg/ml of Puromycin. It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37°C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37°C-CO2 incubator. Monitor the cell viability by counting cells daily for 1~3 days until cells completely recover viability as cells are doubling daily. Once cells are over 90% confluent, harvest cells by centrifugation and passage cells. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, transfer cells to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.

Functional validation:

A. Response of NF-kB – Jurkat cells to phorbol 12-myristate 13-acetate (PMA).1. Harvest NF-kB – Jurkat cells and seed cells into a white solid-bottom 96-well microplate in 100 µl of growth medium at 2.5 x 10^5 cells/well. 2. Right after plating cells, stimulate cells with various concentrations of PMA and incubate cells at 37°C in a CO2 incubator for 6-16 hours. 3. Add 50 µl of luciferase assay reagent per well. 4. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer.

Validation Images & Assay Conditions

Gene/Protein Information For NF-kB (Source: Uniprot.Org, NCBI)

Uniprot ID

Gene ID

Gene Name


Full Name


*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NF-kB , check out the NF-kB Infographic

NF-kB  infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NF-kB : database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for RC1042

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used NF-kB Luciferase Reporter-Jurkat Cell Line?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review NF-kB Luciferase Reporter-Jurkat Cell Line

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for NF-kB Luciferase Reporter-Jurkat Cell Line


Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Product Categories

Primary Antibodies

Primary and Secondary Antibodies

Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!



More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.


Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

Boster Kit


Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

In stock
Order Product



Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.